Tuesday, September 6, 2005

Tuesday, September 6, 2005
7:30 AM–7:00 PM
Market Street Foyer

REGISTRATION OPEN

Tuesday, September 6, 2005
7:00 AM–8:30 AM

PUBLIC RELATIONS COMMITTEE

(Marina Room)

DNA REPAIR SPECIAL INTEREST GROUP

(Seacliff C/D)

RISK ASSESSMENT SPECIAL INTEREST GROUP

(Seacliff A/B)

Tuesday, September 6, 2005
8:30 AM–9:15 AM
Grand Ballroom

PLENARY LECTURE—MARY-CLAIRE KING

Introduction

Wagida Anwar, President of the Pan-African Environmental Mutagen Society (PAEMS), Ain Shams University, Cairo, Egypt

A GENOMIC VIEW OF HUMAN HISTORY

Mary-Claire King, University of Washington, Seattle, WA, United States

Tuesday, September 6, 2005
9:15 AM–10:00 AM
Grand Ballroom

PLENARY LECTURE—ALAIN SARASIN

Introduction

Micheline Kirsch-Volders, President of the European Environmental Mutagen Society, Free University, Brussels, Belgium

#455 MOLECULAR MECHANISMS OF SUNLIGHT-INDUCED SKIN CANCER

Alain Sarasin, Institut Gustave-Roussy, Villejuif, France

Tuesday, September 6, 2005
10:00 AM–10:30 AM
Grand Ballroom Foyer

REFRESHMENT BREAK

Tuesday, September 6, 2005
10:30 AM–12:30 PM
Grand Ballroom A

CURRENT ISSUES SYMPOSIUM—DNA REPAIR

CHROMATIN DYNAMICS: INFLUENCE ON GENOME FUNCTION AND DNA DAMAGE RESPONSE

Chairs: Genevieve Almouzni, Curie Institute, Paris, France and
Gary Felsenfeld, NIH, Bethesda, MD, United States

10:30 AM #456 CHROMATIN ASSEMBLY FACTORS, REPLICATION-FORK ARRESTS, AND DNA- DAMAGE RESPONSE

Genevieve Almouzni, Curie Institute, Paris, France

11:00 AM #457 CHROMATIN BOUNDARIES, EPIGENETIC SIGNALS, AND THE REGULATION OF GENE EXPRESSION

Gary Felsenfeld, NIH, Bethesda, MD, United States

11:30 AM #458 HISTONE PHOSPHORYLATION LINKS ATP- DEPENDENT CHROMATIN REMODELING WITH DNA DAMAGE REPAIR

Haico van Attikum, Miescher Institute Biomedical Research, Basel, Switzerland

11:50 AM #459 INO80 CHROMATIN REMODELING AND DNA REPAIR

Xuetong Shen, University of Texas M.D. Anderson Cancer Center, Science Park–Research Division, Smithville, TX, United States

12:10 PM #460 ROLE OF HISTONE H4 LYSINE 16 ACETYLATION IN MAMMALIAN CELLS

Asifa Akhtar, EMBL, Heidelberg, Germany

Tuesday, September 6, 2005
10:30 AM–12:30 PM
Seacliff C/D

CURRENT ISSUES SYMPOSIUM—ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

ENVIRONMENTAL AND GENETIC FACTORS INFLUENCING THE RISK OF COMMON NONCANCER DISEASE

Chairs: Harvey Mohrenweiser, University California, Irvine, CA, United States and Irene M. Jones, Lawrence Livermore National Laboratory, Livermore, CA, United States

10:30 AM #461 CARDIOVASCULAR DISEASE/HYPERTENSION: GENETIC SUSCEPTIBILITY AND EXPOSURE FACTORS

Daniel T. O’Connor, University California, San Diego, La Jolla, CA, United States

11:00 AM #462 ENVIRONMENT AND GENES IN THE ETIOLOGY OF PARKINSONISM

Caroline M. Tanner, Parkinson’s Institute, Sunnyvale, CA, United States

11:30 AM #463 ROLE OF EXPOSURE AND GENETICS IN INFLAMMATORY/AUTOIMMUNE DISEASE SUCH AS MULTIPLE SCLEROSIS

Lisa F. Barcellos, University California, Berkeley, CA, United States

11:50 AM GENETICS AND EXPOSURE IN THE RISK OF ASTHMA AND RESPIRATORY DISEASE

John R. Balmes, University of California, San Francisco, CA, United States

12:10 PM #464 CHALLENGES IN THE STUDY OF GENE BY ENVIRONMENT INTERACTION IN RISK OF COMMON DISEASES

Harvey Mohrenweiser, University California, Irvine, CA, United States

Tuesday, September 6, 2005
10:30 AM–12:30 PM
Bayview

CURRENT ISSUES SYMPOSIUM—ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

ENVIRONMENTAL MUTAGENS IN AIR

Chairs: Radim J. Sram, Institute of Experimental Medicine, Prague, Czech Republic and
Larry D. Claxton, US EPA, Research Triangle Park, NC, United States

10:30 AM #465 OVERVIEW OF THE MUTAGENICITY OF URBAN AIR

Larry D. Claxton, US EPA, Research Triangle Park, NC, United States

11:00 AM #466 BIOMARKERS OF URBAN AIR-ASSOCIATED MUTAGENICITY

Radim J. Sram, Institute of Experimental Medicine, Prague, Czech Republic

11:30 AM #467 ADDUCTS AND OXIDATIVE DAMAGE OF URBAN AIR

Peter B. Farmer, University of Leicester, Leicester, United Kingdom

11:50 AM #468 AIR POLLUTION AND RISKS FOR CANCER, REPRODUCTIVE EFFECTS, AND CARDIOVASCULAR DISEASE

Joellen Lewtas, University Washington, Seattle, WA, United States

12:10 PM #469 AIR POLLUTION AND CHRONIC HEALTH EFFECTS: THE BAD AND THE WORSE

Lilian Calderón-Garcidueñas, Instituto Nacional de Pediatria, Mexico City, Mexico and Department of Biomedical Pharmaceutical Sciences, University of Montana, Missoula, MT, United States

Tuesday, September 6, 2005
10:30 AM–12:30 PM
Grand Ballroom B

CURRENT ISSUES SYMPOSIUM—ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

MUTAGENS AND CARCINOGENS FROM COOKED FOOD

Chairs: James S. Felton, Lawrence Livermore National Laboratory, Livermore, CA, United States and Nigel J. Gooderham, Imperial Cancer Research Institute, London, United Kingdom

10:30 AM #470 METABOLISM AND GENOTOXICITY OF HETEROCYCLIC AMINES

James S. Felton, Lawrence Livermore National Laboratory, Livermore, CA, United States

11:00 AM #471 CARCINOGENICITY OF MUTAGENS/CARCINOGENS FROM COOKED FOOD

Hitoshi Nakagama, National Cancer Center Research Institute, Tokyo, Japan

11:30 AM #472 HETEROCYCLIC AMINES IN COOKED MEATS IN THE SINGAPORE CHINESE DIET

Adeline Seow, University of Singapore, Singapore

11:50 AM #473 IMPACT OF CHEMOPREVENTION ON THE METABOLISM AND GENOTOXICITY OF HETEROCYCLIC AMINES

Nigel J. Gooderham, Imperial Cancer Research Institute, London, United Kingdom

12:10 PM #474 RISK ASSESSMENT OF ACRYLAMIDE IN FOODS

Jan Alexander, National Institute Public Health, Oslo, Norway

Tuesday, September 6, 2005
10:30 AM–12:30 PM
Seacliff A/B

CURRENT ISSUES SYMPOSIUM—STUDENT/FACULTY PROGRAMS

SCIENCE EDUCATION IN THE UNIVERSITY: NEW TEACHING METHODS

Chairs: James M. Gentile, Research Corporation, Tucson, AZ, United States and
Jo Handelsmann, University Wisconsin, Madison, WI, United States

Sponsored by Research Corporation

10:30 AM #475 BIOLOGY 2010: WHAT DOES THE FUTURE HOLD FOR SCIENCE EDUCATION?

James M. Gentile, Research Corporation, Tucson, AZ, United States

11:00 AM #476 THE HHMI PROFESSORS PROGRAM: SUPPORTING SCIENTISTS TO GET DEEPLY INVOLVED IN SCIENCE EDUCATION

Peter J. Bruns, HHMI and Cornell University, Ithaca, NY, United States

11:30 AM #477 ACTIVE-ENGAGEMENT TEACHING IN LARGE CLASSES: LECTURING LESS WITHOUT SACRIFICING CONTENT

William B. Wood, University of Colorado, Boulder, CO, United States

11:50 AM #478 SCIENTIFIC TEACHING

Jo Handelsman, University of Wisconsin, Madison, WI, United States

12:10 PM #479 REDEFINING SCIENCE EDUCATION AT ALL LEVELS

Bruce M. Alberts, University of California, San Francisco, CA, United States

Tuesday, September 6, 2005
1:00 PM–3:00 PM
Pacific Concourse

POSTERS ATTENDED AND EXHIBITS OPEN

RESPONSES TO ENVIRONMENTAL AGENTS

Odd numbered posters will be attended from 1:00 PM–2:00 PM and even numbered posters will be attended from 2:00 PM–3:00 PM.

#480 ANALYSIS OF LUNG DNA ALTERATIONS AND URINARY 8-HYDROXYDEOXYGUANOSINE IN WISTAR RATS EXPOSED TO A CONSTANT FLOW OF DIESEL ENGINE EXHAUST. Aaltonen, K¹, Svoboda, P², Rynö, M¹, Bion, A³, Dionnet, F⁴, Tamminen, N¹, Harri, M¹, Kasai, H², Morin, JP³, Savela, K¹. ¹Institute of Occupational Health, Helsinki, Finland, ²University of Occupational and Environmental Health, Kitakyushu, Japan, ³INSERM, Rouen, France, ⁴CERTAM, Saint Etienne du Rouvray, France.

#481 INTER-LABORATORY EVALUATION OF THE BIOLUMINESCENT SALMONELLA REVERSE MUTATION ASSAY USING 10 MODEL CHEMICALS. Ackerman, J¹, Hayashi, T², Hitchcock, J⁴, Li, L³, Lu, S³, Nagai, Y², Spence, F⁴, Aubrecht, J¹. ¹Pfizer Inc., Groton, CT, United States, ²Pfizer Inc., Nagoya, Japan, ³Pfizer Inc., La Jolla, CA, United States, ⁴Pfizer Inc., Sandwich, United Kingdom.

#482 CYP1A1 AND GSTM1 GENETIC POLYMORPHISMS IN LUNG CANCER POPULATIONS EXPOSED TO ARSENIC IN DRINKING WATER. Adonis, M, Marin, P, Martinez, V, Gil, L. ¹University of Chile, Santiago, Chile, ²Antofagasta´s Hospital, Antofagasta, Chile, ³University of Chile, Santiago, Chile, ⁴University of Chile, Santiago, Chile.

#483 THE POTENT CARCINOGEN 7,12-DIMETHYLBENZ[a]ANTHRACENE INDUCES TUMORS IN THE RAT MAMMARY GLAND BUT NOT IN THE UTERUS: EFFECTS OF PHYTOESTROGENS, DAIDZEIN AND GENISTEIN SUPPLEMENTATION. Aidoo, A, Bishop, ME, Shelton, SD, Lyn-Cook, LE, Manjanatha, MG. FDA/NCTR, Jefferson, AR, United States.

#484 ANTIMUTAGENIC ACTIVITY OF EXTRACT OF KIWI FRUITS ON MNNG-INDUCED MUTAGENESIS. Akbarova, GH. Baku State University, Baku, Azerbaijan.

#485 RESPONSES OF LYMPHOCYTES FROM ASIAN AND CAUCASIAN DIABETIC PATIENTS AND NON-DIABETICS TO HYDROGEN PEROXIDE AND SODIUM NITRITE AND 2-AMINO-3.8-DIMETHYLIMIDAZO[4,5-F] QUINOXALINE (ME1QX) IN THE COMET ASSAY. Wyatt, NP¹, Kelly, C², Fontana, V³, Merlo, DF³, Whitelaw, D², Anderson, D¹. ¹University of Bradford, Yorkshire, United Kingdom, ²Bradford Royal Infirmary, Yorkshire, United Kingdom, ³Istutio Nazionale per la Ricerca sul Cancro, Genova, Italy.

#486 IN VIVO MUTAGENICITY OF DIESEL EXHAUST AND ITS COMPONENTS, BENZO[a]PYRENE AND 1,6-DINITROPYRENE, IN THE LUNGS OF gpt DELTA MICE. Aoki, Y¹, Hashimoto, AH¹, Amanuma, K¹, Hiyoshi, K², Yanagisawa, R¹, Takano, H¹, Masumura, K³, Nohmi, T³. ¹National Institute for Environmental Studies, Tsukuba, Japan, ²University of Tsukuba, Tsukuba, Japan, ³National Institute of Health Sciences, Tokyo, Japan.

#487 CYTOGENETIC EFFECTS OF THE OCCUPATIONAL EXPOSURE RN-222 AND ITS DECAY PRODUCTS AMONG URANIUM MINERS FROM ONE URANIUM MINE IN BULGARIA. Apostolova, DB¹, Hadjidekowa, W², Paskalev, ZD². ¹Clinic of Occupational Diseases, Medical University, Sofia, Bulgaria, ²National Center of Radiobiology and Radiation Protection, Sofia, Bulgaria.

#488 INHIBITORY EFFECTS OF HETEROCYCLIC AMINE - INDUCED MUTATION AND DNA-ADDUCT FORMATION IN MOUSE LIVER, LUNG AND COLON BY BEER. Arimoto-Kobayashi, S, Takata, J, Nakandakari, N, Fujioka, R, Konuma, T. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

#489 THE MUTAGENIC AIR POLLUTANT 3-NITROBENZANTHRONE FORMS DNA ADDUCTS AFTER REDUCTION BY NQO1 AND CONJUGATION BY NAT AND SULT IN HUMAN HEPATIC CYTOSOLS. Arlt, VM¹, Stiborova, M², Henderson, CJ³, Osborne, MR¹, Bieler, CA⁴, Frei, E⁴, Martinek, V², Sopko, B², Wolf, CR³, Schmeiser, HH⁴, Phillips, DH¹. ¹Institute of Cancer Research, Sutton, Surrey, United Kingdom, ²Charles University, Prague, Czech Republic, ³Biomedical Research Centre, Dundee, United Kingdom, ⁴German Cancer Research Center, Heidelberg, Germany.

#490 CSGMT/JEMS/MMS COLLABORATIVE STUDY FOR THE SKIN MICRONUCLEUS ASSAY. Asano, N¹, Nishikawa, T², Kasamatsu, T³, Gibson, D⁴, Aardema, MJ⁴, Hayashi, M⁵. ¹Nitto Denko Corp., Osaka, Japan, ²Lion Corp., Odawara, Japan, ³Kao Corp., Tochigi, Japan, ⁴P&G, Cincinnati, United States, ⁵National Institute of Health Sciences, Tokyo, Japan.

#491 SUSCEPTIBILITY TO AND INTERVENTION OF CERVICAL CANCER. Au, WW, Salama, SA, Sierra-Torres, CH. University of Texas Medical Branch, Galveston, TX, United States.

#492 THE EFFECTS OF Gadd45? ON PCNA REGULATED NUCLEOTIDE EXCISION REPAIR IN RESPONSE TO UVB DAMAGE. Babiuk, JD, Tron, VA. University of Alberta, Edmonton, Alberta, Canada.

#493 P53-ASSOCIATED TRANSCRIPTION IN HUMAN DERMAL FIBROBLASTS AND HEPG2 CELLS IN RESPONSE TO THE CYANOBACTERIAL TOXIN CYLINDROSPERMOPSIN. Bain, PA¹, Shaw, GR², Patel, BKC¹. ¹The Eskitis Intitute for Cellular and Molecular Therapies, Griffith University, Brisbane, Queensland, Australia, ²National Research Centre for Environmental Toxicology, Brisbane, Queensland, Australia.

#494 EVALUATION AND VALIDATION OF HOUSEKEEPING GENE EXPRESSION IN RESPONSE TO IONIZING RADIATION IN HUMAN CELL LINES. Banda, M, Bommineni, A, Kulkarni, R, Thomas, RA, Tucker, JD. Wayne State University, Detroit, MI, United States.

#495 COMPUTATIONAL CHARACTERIZATION AND PREDICTION OF ESTROGEN RECEPTOR ALPHA COACTIVATOR BINDING INHIBITORS. Bennion, BJ, Kulp, KS, Cosman, M, Lightstone, FC. Lawrence Livermore National Laboratory, Livermore, CA, United States.

#496 GENETIC CONSEQUENCES FROM EXPOSURE TO ROCKET FUEL CHEMICALS EXPERIMENTALLY AND NATURALLY. Kolumbaeva, SJ, Shalakhmetova, TM, Bersimbaev, RI, Begimbetova, D, Umbaev, B. Kazakh National University, Almaty, Kazakhstan.

#497 CYTOGENETIC BIOMONITORING OF A GROUP OF PAKISTANI WORKERS INVOLVED IN PESTICIDE PRODUCTION. Bhalli, JA, Khan, QM. Environmental Toxicology Lab, National Institue for Biotechnology & Genetic Engineering (NIBGE), Faisalabad, Pakistan.

#498 STUDY ON ANTIMUTAGENIC POTENTIAL OF MYROBALAN (Terminalia chebula Retz.) FRUIT EXTRACT TOWARDS UV-INDUCED MUTATION. Bhumadhana P¹, Jirarungrungwong P², Ponpanich L², Chulasiri M¹. ¹Mahidol University, Bangkok, Thailand, ²S & J International Enterprises, Bangkok, Thailand.

#499 GLOBAL TRANSCRIPTIONAL RESPONSE TO IONIZING RADIATION IS ATM-DEPENDENT. Culligan, KM, Robertson, C, Britt, AB. University of California, Davis, Davis, CA, United States.

#500 URBAN AIRBORNE PARTICULATE MATTER IS TOXIC TO CHICKEN EMBRYOS. Bueno-Guimarães, HM, Pannuti, CV, Martins, RSL, Garcia, MLB, Santos, RS, Sowmy, T, Saldiva, PHN. Laboratory of Experimental Air Pollution - School of Medicine - University of São Paulo, São Paulo/São Paulo, Brazil.

#501 EFFECTS OF SCUTELLARIA BAICALENSIS RADIX HERBAL ACUPUNCTURE SOLUTION (SBRHA) ON THE ACUTE TOXICITY. Byun, BH¹, Park, EJ². ¹Dept. of Oriental Medicine, Daegu Haany University, Daegu, South Korea, ²Dept. of Food and Nutrition, Kyungnam University, Masan, South Korea.

#502 GENE EXPRESSION PROFILES IN THE EARLY STAGE OF BALB/C-3T3 CELLS TRANSFORMATION PROMOTED WITH 12-O-TETRADECANOYLPHORBOL 13-ACETATE. Ao, L¹, Yang, M², Cao, J¹. ¹Preventive Medical College,Third Military Medical University, Chongqing 400038, China, ²Biochemistry Department, Hong Kong, China.

#503 SEASONALITY OF THE MUTAGENIC AND CYTOTOXIC RESPONSE IN AN URBAN AND RURAL AREA IN PORTO ALEGRE, RS, BRAZIL. Cardozo, TR, Rosa, DP, Pastoriza, T, Rocha, JV, Pereira, TS, Vargas, VMF. Programa de Pesquisas Ambientais, Fundação Estadual de Proteção Ambiental Henrique Luis Roessler, FEPAM, Porto Alegre, RS, Brazil.

#504 SKIN CANCER RISK CORRELATION WITH INCIDENT ULTRAVIOLET RADIATION IN THE DISTRITO FEDERAL - BRAZIL. Neto, JQ, Castro, CFS, Baptista, GMM, Zara, LF. Catholic University at Brasília, Brasília, DF, Brazil.

#505 GENOTOXIC RESPONSE OF PATERNALLY GAMMA RAYS EXPOSED BALB-C MICE TO RADIATION AND CHEMICAL MUTAGENS. Bhilwade, HN, Chaubey, RC. Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.

#506 ELEVATED AMBIENT SUMMER O3 IS LINKED TO CYTOGENETIC DAMAGE IN ORAL EPITHELIA OF HEALTHY COLLEGE STUDENTS. Chen, C, Tran, C, Chen, M, Qin, H, Balmes, J, Tager, IB, Holland, N. University of California, Berkeley, CA, United States.

#507 INDUCTION OF DELAYED CHROMOSOMAL INSTABILITY IN NORMAL HUMAN FIBROBLAST CELLS BY LOW DOSE RADIATION. Cho, YH¹, Woo HD¹, Ha SW ², Chung HW¹. ¹School of Public Health, Seoul National University, Seoul, Korea, ²College of Medicine, Seoul National University, Seoul, South Korea.

#508 BENZIDINE AND ITS ANALOGUES INDUCE OXIDATIVE GENOTOXICITIES. Chung, K-T, Makena, P. The University of Memphis, Memphis, TN, United States.

#509 METABOLIC ACTIVATION OF DIMETHYLNITROSAMINE AND AFLATOXIN B1 WITH HUMAN LIVER S-9 IN THE MOUSE LYMPHOMA FORWARD MUTATION ASSAY. Hew, K¹, Cifone, MA², Rundell, J³. ¹Purdue Pharma L.P., Ardsley, NY, United States, ²Covance Laboratories Inc., Vienna, VA, United States, ³Molecular Toxicology Inc., Boone, NC, United States.

#510 THE BENEFITS OF USING PHARMACO- AND TOXICOGENOMICS IN DRUG THERAPIES. Clark, LS, Murphy, MP. Gentris Corporation, Morrisville, NC, United States.

#511 MUTAGENICITY OF CHROMIUM PICOLINATE AND ITS COMPONENTS IN Salmonella typhimurium AND L5178Y MOUSE LYMPHOMA CELLS. Whittaker, P¹, San, RHC², Clarke, JJ², Seifried, HE³, Dunkel, VC¹. ¹Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, United States, ²BioReliance, Rockville, MD, United States, ³National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

#512 COMPARISON OF CELL PREPARATION METHODS FOR THE IN VIVO MOUSE LIVER COMET ASSAY. Clay, P. Syngenta CTL, Macclesfield, Cheshire, United Kingdom.

#513 COMPARATIVE MOUSE GENOMICS CENTERS CONSORTIUM (CMGCC): TO AN IMPROVED UNDERSTANDING OF THE BIOLOGICAL SIGNIFICANCE OF HUMAN POLYMORPHISMS. The CMGCC Steering Committee. NIEHS, Research Triangle Park, NC, United States.

#514 USING CO-REGULATION TO UNDERSTAND IONIZING RADIATION RESPONSIVE GENES AND PATHWAYS. Coleman, MA¹, Xin, X¹, Krefft, A¹, Pearson, F¹, Critchlow, TJ¹, Ovcharenko, I¹, Marchetti, F¹, Nelson, D¹, Krishnan, K¹, Tucker, J², Wyrobek, AJ¹. ¹LLNL, Biosciences directorate, Livermore, CA, United States, ²Department of Biological Sciences, Wayne State University, Detroit, MI, United States.

#515 EFFECT OF AN ENVIRONMENTAL COMPLEX MIXTURE ON PAH-INDUCED CARCINOGENESIS IN THE TWO-STAGE SENCAR MOUSE TUMORIGENESIS MODEL. Courter, LA¹, Musafia, T¹, Fischer, K², Bildfell, R², Giovanini, J³, Pereira, C³, Mahadevan, B¹, Baird, WM¹. ¹Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States, ²College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States, ³Department of Statistics, Oregon State University, Corvallis, OR, United States.

#516 ADAPTIVE RESPONSE IN pKZ1 MOUSE PROSTATE AFTER EXPOSURE TO VERY LOW DOSES OF X-RADIATION. Day, TK¹, Zeng, G¹, Hooker, AM¹, Bhat, M², Sykes, PJ¹. ¹Department of Haematology & Genetic Pathology, Flinders University and Medical Centre, Bedford Park, South Australia, 5042, Australia, ²Department of Medical Physics, Royal Adelaide Hospital, Adelaide, South Australia, 5000, Australia.

#517 MICRONUCLEATED ERYTHROCYTE POPULATIONS: POTENTIAL BIOMARKERS FOR HUMAN DRUG STUDIES AS DEMONSTRATED BY HYDROXYUREA FOR PEDIATRIC SICKLE CELL DISEASE. Dertinger, SD¹, Howard, T², Zimmerman, SA³, Ware, RE². ¹Litron Laboratories, Rochester, NY, United States, ²St. Jude Children’s Research Hospital, Memphis, TN, United States, ³Duke University Medical Center, Durham, NC, United States.

#518 Bacopa monnieri, A WETLAND PLANT, FOR IN VIVO AND IN VITRO ECO-GENOTOXICITY ASSESSMENT USING COMET ASSAY. Vajpayee, P, Shanker, R, Dhawan, A. Industrial Toxicology Research Centre, Lucknow, Uttar Pradesh, India.

#519 EFFECT OF P53 HAPLODEFICIENCY ON MICRONUCLEUS INDUCTION AND HPRT MUTANT FREQUENCY IN B6C3F1 MICE TREATED PERINATALLY WITH AZT AND AZT/3TC. Dobrovolsky, VN¹, Shaddock, JG¹, Bishop, ME¹, Lee, FW¹, Lewis, SM¹, Leakey, JE¹, Dunnick, JK², Aidoo, A¹, Heflich, RH¹. ¹NCTR/FDA, Jefferson, AR, United States, ²NIEHS, Research Triangle Park, NC, United States.

#520 INTEGRATING NEW TECHNOLOGIES INTO THE ASSESSMENT OF HERITABLE GENETIC EFFECTS. Elespuru, RK. FDA, Silver Spring, MD, United States.

#521 IN VIVO GENOTOXICITY OF THE SYNTHETIC PYRETHROID PESTICIDE “CYPERMETHRIN” IN RAT LIVER CELLS BY COMET ASSAY. El-Khatib, HN, Abdel-Aziz, M, Badr, Y, Kamal, N. Central Agricultural Pesticides Laboratory, Giza, Egypt.

#522 PBDEs OR PCBs WITH PERFLUORINATED COMPOUNDS: SYNERGY IN THE INDUCTION OF SE-GLUTATHIONE PEROXIDASE, CELL CYCLE ARREST, AND CYTOTOXICITY IN HUMAN HEPG2 CELLS. Esch, HL, Ludewig, G. University of Iowa, Iowa City, IA, United States.

#523 COMPARISON OF CELL TRANSFORMATION ASSAY AND ALKALINE COMET ASSAY USING SYRIAN HAMSTER EMBRYO (SHE) CELLS. Escobar, PA¹, Pant, K¹, Gibson, DP², Aardema, MJ², San, RHC¹. ¹Genetic Toxicology Department, BioReliance, Rockville, MD, United States, ²The Procter & Gamble Company, Cincinnati, OH, United States.

#524 N-NITROSODIETHYLAMINE MUTAGENICITY AT LOW CONCENTRATION. Felzenszwalb, I, Ribeiro Pinto, LF, Aiub, CAF. Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

#525 EXPLORE THE ACTIVATED CELLULAR PATHWAYS IN RESPONSE TO DNA DAMAGE BY UV RADIATION. Feng, J, Xue, F, Li, W, Wen, L. Dept. of Chemistry, Temple University, Philadelphia, PA, United States.

#526 POSSIBLE ANTI-MUTAGEN ACTION OF ORANGE JUICE AND VITAMIN C ON THE DNA DAMAGE CAUSED ON MICE BLOOD CELLS IN VIVO BY CYCLOPHOSPHAMIDE, METHYL METHANESULFONATE, FESO4 AND CUSO4. Franke, SIR¹, Prá, D¹, Erdtmann, B², Da Silva, J¹, Henriques, JAP³. ¹PPG em Nutrição Clínica/Curso de Nutrição/DEDFIS – UNISC; PPGBCM/PPGBM – UFRGS, Santa Cruz do Sul; Porto Alegre, RS, Brazil, ²Centro de Biotecnologia – UCS, Caxias do Sul, RS, Brazil, ³Curso de Biologia/Curso de Farmácia – ULBRA, Canoas, RS, Brazil.

#527 INHIBITION OF RAS/RHO SIGNALING BY STATINS IMPAIRS RADIATION-INDUCED STRESS RESPONSES AND INCREASES RADIORESISTANCE. Fritz, G, Nuebel, T, Damrot, J, von Bardeleben, R, Kaina, B. Department of Toxicology, Mainz, Germany.

#528 MODIFICATION OF INDUCED CYTOCHROME P450 1A1 IN RAINBOW TROUT BY POLYCHLORINATED BIPHENYLS (PCB) IN FISH-DIET. Ghanem, M¹, Salem, M², Yao, J², Rexroad, C³, Nath, J⁴. ¹Department of Animal Medicine, College of Veterinary Medicine, Benha University, Egypt, ²Department of Animal Science, West Virginia University, Morgantown, WV, United States, ³National Center for Cool and Cold Water Aquaculture, Kearneysville, WV, United States, ⁴Genetics and Developmental Biology Program, West Virginia University, Morgantown WV, United States.

#529 A SYSTEMATIC SCREEN OF THE YEAST Saccharomyces cerevisiae DELETION MUTANT COLLECTION FOR NOVEL GENES REQUIRED FOR DNA-DAMAGE INDUCED MUTAGENESIS. Gong, J, Kim, E, Siede, W. University of North Texas Health Science Center, Fort Worth, TX, United States.

#530 IONIZING RADIATION CAUSES A DOSE-DEPENDENT INDUCTION OF ATM SER1981 PHOSPHORYLATION IN THE JUVENILE RAT BRAIN. Gorodetsky, E, Brooks, PJ. NIAAA NIH, Rockville, MD, United States.

#531 VARIABILITY IN BONE MARROW MUTATIONAL RESPONSE IN BREAST CANCER PATIENTS TREATED WITH GENOTOXIC CHEMOTHERAPY. Grant, SG, Kelly III, JL, Vogel, VG, Brufsky, AM, Bigbee, WL, Latimer, JJ. University of Pittsburgh, Pittsburgh, PA, United States.

#532 ASSESSMENT OF EXPOSURE TO ORGANIC SOLVENTS AND GENOTOXIC EFFECTS IN PAINT INDUSTRY WORKERS. Groot, H¹, Varona, M², Patiño, RI³, Sicard, D¹, Torres, MM¹, Cárdenas, O², Pardo, D². ¹Universidad de los Andes, Bogotá, Colombia, ²Instituto Nacional de Salud, Bogotá, Colombia, ³Universidad El Bosque, Bogotá, Colombia.

#533 GUT MYOELECTRICAL ACTIVITY INDUCES HEAT SHOCK PROTEINS IN E. coli AND CACO-2 CELLS AND MODIFIES APOPTOSIS. Grzesiuk, E¹, Sikora, A¹, Lubanska, A², Wolinski, J², Zabielski, R², Laubitz, D². ¹Institute of Biochemistry&Biophysics PAS, Warszawa, Poland, ²The Kielanowski Institute of Animal Physiology & Nutrition PAS, Jablonna, Poland.

#534 DISTINCTIVE ALTERATION IN GENE EXPRESSION PROFILES BY BOTANICAL CARCINOGENS IN LIVER AND KIDNEY OF RATS. Guo, L¹, Zhang, L², Shi, L¹, Mei, N³, Sun, Y², Fung, C², Wicki, R², Cassel, J², Dragan, Y¹, Chen, T³. ¹Division of Systems Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR, United States, ²Arrays and SDS Application Group, Applied Biosystems, Foster City, CA, United States, ³Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, FDA, Jefferson, AR, United States.

#535 COMET ASSAY AS A POSSIBLE MEAN TO DIFFERENTIATE GENOTOXINS FROM CYTOTOXINS THAT PRODUCE CHROMOSOMAL DAMAGE IN VITRO. Hashizume, T, Nakajima, Y, Horinouchi, M. Takeda Pharmaceutical Company Limited, Osaka, Japan.

#536 FLOW CYTOMETRIC DETECTION OF CHEMICALLY INDUCED TANDEM REPEAT MUTATIONS IN TWO MURINE CELL LINES. Healy, C¹, Wade, M², McMahon, A², Johnson, D³, Parfett, C¹. ¹Mutagenesis Section, Environmental Health Sciences Bureau, HECSB, Health Canada, Ottawa, ON, Canada, ²Systemic Toxicology and Pharmacokinetics Section, Environmental Health Sciences Bureau, HECSB, Health Canada, Ottawa, ON, Canada, ³Biology Department, University of Ottawa, Ottawa, ON, Canada.

#537 GENOTOXIC EFFECTS OF DIESEL EXHAUST PARTICLE EXTRACT IN NIH/3T3 CELLS. Heo, C¹, Kim, NY¹, Chung, KH², Moon, CK³, Heo, MY¹. ¹Kangwon National Univ, Chunchon, South Korea, ²Sungkyunkwan University, Suwon, South Korea, ³Seoul National University, Seoul, South Korea.

#538 PREVENTIVE EFFECTS OF METALLOTHIONEIN ON DNA AND LIPID METABOLISM DAMAGE CAUSED BY CHRONIC MILD STRESS DUE TO FASTING AND RESTRAINT IN HYPERLIPIDEMIC MICE. Higashimoto, M¹, Ishibashi, S², Isoyama, N², Suzuki, S², Takiguchi, M³, Ogawa, N⁴, Umehara, A⁴, Tsuda, TT⁵, Ohnishi, Y⁵, Sato, M². ¹Dep. Food and Nutrition, Suzugamine Women’s College, Hiroshima, Japan, ²Fac. Pharm. Sci., Tokushima Bunri Univ., Tokushima, Japan, ³Fac. Pharm. Sci., Hiroshima Internat. Univ., Hiroshima, Japan, ⁴Fac. Human Lfe Sci., Tokushima Bunri Univ., Tokushima, Japan, ⁵Univ. Tokushima Grad. Sch., Tokushima, Japan.

#539 POSSIBLE ROLES OF GLUTATHIONE PEROXIDASE 1 POLYMORPHISM ON THE RISK FOR LUNG CANCER IN SMOKERS AND NONSMOKERS. Ryk, C, Hou, YS, Lambert, B, Hou, S-M. Unit of Environmental Medicine, Center for Nutrition and Toxicology, NOVUM, S-141 57, Huddinge, Sweden.

#540 GENOTOXIC AND CYTOTOXIC EFFECTS OF PSYCHOACTIVE DRUGS IN CAUCA (COLOMBIA) CONSUMERS. Hoyos, LS, Carvajal, SM, Ocampo, AP. ¹Universidad del Cauca, Popayán, Cauca, Colombia, ²Universidad del Cauca, Popayán, Cauca, Colombia, ³Colegio San José de Tarbes, Popayán, Cauca, Colombia.

#541 ANALYSIS OF IN SITU DNA BINDING SITES OF P53 AND P73 IN RESPONSE TO HYDROXYUREA. Huang, VH, Kwon, YSK, Fu, XDF, Wang, JYJW. University of California, San Diego, La Jolla, CA, United States.

#542 APPLICATION OF A GEOGRAPHIC INFORMATION SYSTEM (GIS) AS A TOOL TO EXPLORE ASSOCIATIONS BETWEEN AIR POLLUTION AND CYTOGENETIC DAMAGE IN AFRICAN AMERICAN CHILDREN AND ADULTS. Huen, K¹, Gunn, L¹, Duramad, P¹, Jeng, M¹, Johnson, S¹, Scalf, R², Sweeters, N³, Lubin, B³. ¹University of California, Berkeley, School of Public Health, Berkeley, CA, United States, ²Environmental Health Investigations Branch, California Department of Health Services, Oakland, CA, United States, ³Pediatric Clinical Research Center, Children’s Hospital and Research Center at Oakland, Oakland, CA, United States.

#543 BILE ACIDS ARE MUTAGENS, CLASTOGENS AND ANEUGENS IN OESOPHAGEAL CELLS, THROUGH THEIR GENERATION OF ROS. Jenkins, GJS¹, Doak, SH¹, D’Souza, FR¹, Parry, JM¹, Baxter, JN¹, Suzen, HS². ¹Swansea University, Wales, United Kingdom, ²Ankara University, Ankara, Turkey.

#544 COMPARISON OF HYDROQUINONE-INDUCED CHROMOSOME DAMAGE IN TK6 CELLS AND HUMAN LYMPHOCYTES. Ji, Z, Smith, MT, Woo, J, Zhang, L. Molecular Epidemiology and Toxicology Laboratory, School of Public Health, University of California, Berkeley, CA, United States.

#545 GENE EXPRESSION AND DNA ADDUCT FORMATION IS MODULATED BY CHLOROPHYLLIN IN NORMAL HUMAN MAMMARY EPITHELIAL CELLS EXPOSED TO BENZO[a]PYRENE (BP). John, K¹, John, K², Divi, R³, Keshava, C², Orozco, CC³, Whipkey, DL¹, Poirier, MC³, Nath, J², Weston, A¹, Weston, A². ¹Genetics and Developmental Biology Program, West Virginia University, Morgantown, WV, United States, ²Toxicology and Molecular Biology Laboratory, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, United States, ³Carcinogen-DNA Interactions Section, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.

#546 RISK FACTORS FOR ORAL AND ESOPHAGEAL CANCER IN A DEVELOPING COUNTRY: A HOSPITAL-BASED CASE-CONTROL STUDY, NEPAL. Joshi, SD, Pandit, N, Bk, SK. Nepal Medical College and Teaching Hospital, Kathmandu, Nepal.

#547 PHOTOCHEMICAL GENOTOXICITY TESTING IN VITRO: A EUROPEAN COLLABORATIVE STUDY ON THE COMET ASSAY AND THE MICRONUCLEUS TEST. Kasper, P¹, Aeby, P², Brendler-Schwaab, S³, Epe, B⁴, Froetschl, R¹, Hertel, C⁴, Kirchner, S⁵, Meurer, K⁶, Plappert-Helbig, U⁷, Schmidt, E⁸. ¹Federal Institute for Drugs and Medical Devices (BfArM), Bonn, Germany, ²Cosmital SA (Wella AG), Marly, Switzerland, ³Bayer HealthCare (current address 1), Wuppertal, Germany, ⁴University of Mainz, Mainz, Germany, ⁵F. Hoffmann-La Roche, Basel, Switzerland, ⁶RCC Cytotest Cell Research GmbH, Rossdorf, Germany, ⁷Novartis Pharma AG, Basel, Switzerland, ⁸ZEBET, Federal Institute for Risk Assessment (BfR), Berlin, Germany.

#548 TRANSCRIPTIONAL SIGNATURES OF PRIMARY NORMAL HUMAN MAMMARY EPITHELIAL CELLS IN RESPONSE TO DIESEL PARTICULATE EXTRACT DETECTED WITH DNA MICROARRAYS. Keshava, C¹, Keshava, N¹, Whipkey, DL², Weston, A². ¹National Center for Environmental Assessment, Office of Research and Development, US Environmental Protection Agency, Washington, DC, United States, ²Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Morgantown, WV, United States.

#549 METHYLMERCURY INDUCED CELL CYCLE ARREST BY ACTIVATION OF ERK1/2 DURING RETINOIC-ACID DIFFERENTIATION OF HUMAN NEURONAL CELLS. Kim, YJ, Kim, YS, Kim, MS, Ryu, JC. Korea Institute Science & Technology, Seoul, South Korea.

#550 GENOTOXICITY PROFILES OF COMMON ALKYL HALIDES AND ALKYL ESTERS. Kirpnick, Z¹, Osowski, J², Masucci, M², Rubitski, E², Cheung, J², Ackerman, J², Ku, W², Aubrecht, J², Schiestl, RH¹. ¹UCLA, Los Angeles, CA, United States, ²Pfizer, Inc., Groton, CT, United States.

#551 HIGHER FREQUENCY OF GENOME REARRANGEMENTS IN THE PROGENY OF STRESS-TREATED PLANTS: TRANSGENERATIONAL PLANT “BYSTANDER EFFECT”? Boyko, A, Zemp, F, Kovalchuk, I. Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.

#552 ARSENIC AND THE HUMAN DNA POLYMERASE ? AND AP ENDONUCLEASE I PROMOTERS. Kozlovski, RM, Snow, ET. Deakin University, Burwood, Victoria, Australia.

#553 A SURVEY OF LOWEST EFFECTIVE CONCENTRATIONS OF BACTERIAL MUTAGENS: DETECTION OF MUTAGENS AS IMPURITIES IN NONMUTAGENIC SUBSTANCES USING THE AMES ASSAY. Cyr, MO, Cheung, JR, Dobo, KL, Aubrecht, JA, Ku, WW. Pfizer Inc., Groton, CT, United States.

#554 DNA-PROTEIN CROSSLINKS IN ERYTHROCYTES OF GOLDFISH FOLLOWING ACUTE EXPOSURE TO HEXAVALENT CHROMIUM IN TANK WATER. Kuykendall, JR, Miller, KL, Perry, MW, Bradley, MJ, Mullen, MW, Cain, AV, Rohrs, KJ. Raabe College of Pharmacy, Ohio Northern University, Ada, OH, United States.

#555 OXIDATIVE DNA DAMAGE IN PATIENTS WITH GASTRITIS AND GASTRIC CANCER INFECTED BY Helicobacter pylori. Ladeira, MSP¹, Rodrigues, MAM¹, Pinto, CLS¹, Prado, RP¹, Queiroz, DMM², Guerra, J², Salvadori, DMF¹. ¹UNESP, Botucatu, SP, Brazil, ²UFMG, Belo Horizonte, MG, Brazil.

#556 WITHDRAWN

#557 EFFECT OF ESTROGEN RECEPTOR (ER) ON BENZO[a]PYRENE-DNA ADDUCT FORMATION IN HUMAN BREAST CANCER CELLS. Lee, BM, Kang, SC. Sungkyunkwan University, Suwon, South Korea.

#558 MUTAGENICITY OF THE MYCOTOXIN ALTERNARIOL IN CULTURED MAMMALIAN CELLS. Brugger, EM, Podlech, J, Metzler, M, Lehmann, L. University of Karlsruhe, Karlsruhe, Germany.

#559 BIOMONITORING OF GENOTOXIC EFFECTS USING COMET ASSAY IN NATIVE FISH FROM SINOS RIVER, RS, BRAZIL. Lemos, CT, Lubianca, JM, Oliveira, NCD. Divisão de Biologia, Programa de Pesquisas Ambientais, Fundação Estadual de Proteção Ambiental Henrique Luís Roessler - FEPAM, Avenida Dr. Salvador França, 1707, CEP 90690-000., Porto Alegre, RS, Brazil.

#560 EVALUATION OF THE POTENTIAL FOR RENAL FAILURE AND URINARY TRACT CARCINOMA IN POPULATIONS EXPOSED TO BOTANICALS CONTAINING ARISTOLOCHIC ACID. Levy, DD. US Food & Drug Administration, College Park, MD, United States.

#561 EXPLORING THE RELATIONSHIP BETWEEN POLYMORPHISM OF CYSTATHIONINE BETA SYNTHASE GENE AND CONGENITAL HEART DISEASE IN CHINESE NUCLEAR FAMILIES. Li, Y. Peking University, Beijing, China.

#562 CELLULAR AND SUBCELLULAR DISTRIBUTIONS OF RADIATION-INDUCED TRANSCRIPT AND PROTEIN EXPRESSION AFTER LOW-DOSE IRRADIATION OF BRAIN TISSUE. Lowe, X, Marchetti, F, Lu, X, Ahn, S, Wyrobek, AJ. Biosciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, United States.

#563 EVALUATING LIVER DNA FROM BIG BLUE MICE TREATED WITH ACRYLAMIDE AND GLYCIDAMIDE FOR MUTATIONS USING THE TET-SELECTION ASSAY. Manjanatha, MG¹, Shelton, SD¹, Ennis, DG², Heflich, RH¹. ¹Food and Drug Administartion, National Center For Toxicological Research, Jefferson, AR, United States, ²University Of Louisiana, Lafatette, LA, United States.

#564 GENOMIC CHANGES IN THE HUMAN TRANSCRIPTOME DEFINE THE CELLULAR RESPONSE TO LOW DOSE EXPOSURES TO IONIZING RADIATION. Manohar, CF, Krishnan, K, Coleman, MA, Pearson, F, Furtado, MR, Nelson, D, Wyrobek, AJ. Lawrence Livermore National Laboratory, Livermore, CA, United States.

#565 CHEMOPROTECTION OF ROOIBOS AND HONEYBUSH HERBAL TEAS AGAINST CANCER PROMOTION INDUCED BY FUMONISIN B1 IN RAT LIVER. Marnewick, JL¹, Van der Westhuizen, FH², Joubert, E⁴, Swanevelder, S¹, Snijman, P¹, Swart, P³, Gelderblom, WCA¹. ¹Medical Research Council, Tygerberg, South Africa, ²Northwest University, Potchefstroom, South Africa, ³Stellenbosch University, Stellenbosch, South Africa, ⁴Agricultural Research Council, Stellenbosch, South Africa.

#566 EVALUATION OF THE MODIFIED COMET ASSAY: THE USE OF LESION SPECIFIC REPAIR ENDONUCLEASES TO DISTINGUISH OXIDATIVE DAMAGE FROM OTHER DNA LESIONS. Martin, EA, Smith, CC, O’Donovan, M. AstraZeneca, Alderley Park, Macclesfield, Cheshire, United Kingdom.

#567 MUTAGENIC POTENTIAL OF CONTROL (OR PRESUMED CONTROL) SOIL SAMPLES. Le Curieux, F², Courty, B¹, Laboudigue, A³, Belkessam, L³, Marzin, D¹. ¹Institut Pasteur de Lille, Lille, France, ²Faculty of Pharmacy, Lille, France, ³National Research Center on Polluted Sites and Soils (CNRSSP), Douai, France.

#568 GENOTOXCITY OF THIOPHENE DERIVATIVES. May, K, Kitching, J, Mason, C, Burlinson, B, Smith, BJ, Hawkins, DR. Huntingdon Life Sciences Ltd, Huntingdon, Cambridgeshire, United Kingdom.

#569 WITHDRAWN

#570 MUTAGENICITY OF TAMOXIFEN INDUCED DNA ADDUCTS IN HUMAN CELLS: EVIDENCE FOR REMOVAL BY THE NUCLEOTIDE EXCISION REPAIR (NER) PATHWAY. McLuckie, KIE¹, Crookston, RJR¹, Gaskell, M¹, Routledge, MN², Martin, EA³, Farmer, PB¹, Brown, K¹. ¹Cancer Biomarkers and Prevention Group, The Biocentre, University of Leicester, Leicester, United Kingdom, ²Molecular Epidemiology Unit, University of Leeds, Leeds, United Kingdom, ³Genetic Toxicology, AstraZeneca, Alderley Park, Macclesfield, United Kingdom.

#571 DOES MULTIGENERATIONAL EXPOSURE TO ENVIRONMENTAL RADIATION (CHORNOBYL, UKRAINE) AFFECT MINISATELLITE VARIATION IN Clethrionomys glareolus. Meeks, HN, Kendall, GC, Wickliffe, JK, Maltbie, M, Hoofer, SR, Rodgers, BE, Chesser, RK, Baker, RJ. Texas Tech University, Lubbock, TX, United States.

#572 THE MUTANT FREQUENCIES AND TYPES OF MUTATIONS INDUCED BY COMFREY IN THE LUNGS OF TRANSGENIC BIG BLUE RATS. Mei, XB¹, Chen, T². ¹Central High School, Little Rock, AR, United States, ²National Center for Toxicological Research, FDA, Jefferson, AR, United States.

#573 REAL-TIME IMAGING OF PHOTO-CONTROLLED DNA POLYMERISATION. Perrins, RD¹, Martin-Fernandez, ML², Tobin, MJ², Wharton, CW¹, Meldrum, RA¹. ¹University Of Birmingham, Birmingham, United Kingdom, ²CCLRC, Daresbury Laboratories, Warrington, Cheshire, United Kingdom.

#574 MUTAGENICITY AND ANTIMUTAGENICITY OF HYDRO-ETHANOLIC AND BOILED EXTRACTS OF TWO CULTIVARS OF IRANIAN SAFFLOWER FLORETS BY SOS/umu ASSAY. Mohseni, M¹, Ohe, T², Azizi, E¹, Ostad, SN¹, Hamedi, MM¹, Shariatpanahi, SM¹, Fazeli, M¹. ¹Tehran University of Medical Sciences-Faculty of Pharmacy, Tehran, Iran, ²Kyoto Women’s University, Kyoto, Japan.

#575 MULTICOLOR SPECTRAL KARYOTYPING OF THE L5178Y/Tk^+/--3.7.2C MOUSE LYMPHOMA CELL LINE. Sawyer, JR¹, Binz, RL¹, Wang, J², Moore, MM². ¹Cytogenetics Laboratory, Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR, United States, ²Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, Jefferson, AR, United States.

#576 ELUCIDATING THE SIGNIFICANCE OF POLYPLOIDY INDUCTION IN THE HUMAN LYMPHOCYTE CHROMOSOMAL ABERRATION ASSAY BY FLOW CYTOMETRY. Muehlbauer, P, Spellman, R. Pfizer Global R&D, Groton, CT, United States.

#577 THE ROLE OF CASPASE 2 IN DNA MISMATCH REPAIR-DEPENDENT APOPTOSIS AFTER DNA DAMAGE. Narine, KD, Young, LC, Tron, VA, Andrew, SE. University of Alberta, Edmonton, Alberta, Canada.

#578 ISOLATION AND CHARACTERIZATION OF ANTIMUTAGENIC FACTOR(S) IN THE EDIBLE MUSHROOM Agrocybe cylindracea. Shiozawa, A, Taira, K, Miyashita, Y, Okamoto, K, Arimoto, S, Negishi, T. Okayama University, Okayama, Japan.

#579 THE EMERGENCE OF MUTAGENIC MECHANISMS IN PATIENTS WITH INFLAMMATORY BOWEL DISEASE. Nguyen, TN, Colletti, RC, O’Neill, PO, Messier, TM, Finette, BF. University of Vermont, Burlington, United States.

#580 IDENTIFICATION OF ENVIRONMENTAL SENESCEGENS USING A S. cerevisiae SCREENING ASSAY. Olaharski, AJ, Rine, J, Babiarz, J, Zhang, L, Smith, MT. University of California, Berkeley, Berkeley, CA, United States.

#581 GENERATION OF MUTAGENIC COMPOUNDS FROM THE REACTION OF AZO DYES AND CHLORINE DURING THE PRODUCTION OF DRINKING WATER. Oliveira, DPO¹, Carneiro, PAC², Rech, CMR³, Zanoni, MVBZ², Umbuzeiro, GAU³. ¹Universidade de São Paulo, São Paulo, SP, Brazil, ²Universidade Estadual de São Paulo, Araraquara, Brazil, ³Companhia de Tecnologia de Saneamento Ambiental, São Paulo, Brazil.

#582 INDUCTION OF SISTER-CHROMATID EXCHANGES AND CHROMOSOMAL ABERRATIONS BY ACRYLAMIDE AND GLYCIDAMIDE. Martins, C¹, Gaspar, J¹, Martins, V¹, Gamboa da Costa, G², Marques, MM², Rueff, J¹, Oliveira, NG³. ¹Dep. Genetics, Faculty of Medical Sciences, New University of Lisbon, Lisbon, Portugal, ²Centro de Química Estrutural, Instituto Superior Técnico, Lisbon, Portugal, ³Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal.

#583 CONTINUOUS EXPOSURE OF MICE TO NON-RADIOACTIVE CESIUM CHLORIDE INDUCES DNA BREAKAGE AND APOPTOSIS PROCESSES IN SPLEEN CELLS. Osipov, AN, Elakov, AL. Scientific and Industrial Association “Radon”, Moscow, Russia.

#584 IS X-RAY IRRADIATION OF FEEDER CELLS NECESSARY FOR SYRIAN HAMSTER EMBRYO (SHE) CELL TRANSFORMATION ASSAY? Pant, K, Sly, JE, Emory, BR, Bruce, SW, San, R. BioReliance, Invitrogen bioservices, Rockville, MD, United States.

#585 EFFECT OF Bacillus polyfermenticus SCD SUPPLEMENTATION ON ANTIOXIDANT SYSTEM AND COLON CARCINOGENESIS IN F344 MALE RAT. Park, EJ¹, Park, JS², Byun, BH³, Paik, HD². ¹Dept. of Food and Nutrition, Kyungnam University, Masan, South Korea, ²Div. of Animal Life Science, Konkuk University, Seoul, South Korea, ³Dept. of Oriental Medicine, Daegu Haany University, Daegu, South Korea.

#586 ASSOCIATION BETWEEN ENVIRONMENTAL AND HUMAN MONITORING IN IMPACT AREAS REGARDING THE RELEASE OF GENOTOXINS: PRELIMINARY DATA. Pereira, TS², Salvadori, DMF³, Vargas, VMF¹. ¹Programa de Pesquisas Ambientais, Fundação Estadual de Proteção Ambiental Henrique Luis Roessler, Porto Alegre, RS, Brazil, ²Pós-graduação em Ecologia- Universidade Federal do Rio Grande do Sul – UFRGS, Porto Alegre, RS, Brazil, ³Universidade Estadual Paulista – UNESP, Botucatu, SP, Brazil.

#587 EFFECTS OF SKIN METABOLISM ON THE GENOTOXIC POTENTIAL OF SELECTED HAIR DYES. Pfuhler, S¹, Zeller, A². ¹Wella AG, Darmstadt, Germany, ²Cosmital SA, Marly, Switzerland.

#588 CYP2E1 AND CYP3A4 SINGLE NUCLEOTIDE POLYMORPHISMS AND RELATIONSHIPS TO mRNA LEVELS IN PERIPHERAL BLOOD LYMPHOCYTES. Phillips, S, Previte, R, Blanton, H, Clark, S. Gentris Corporation, Morrisville, NC, United States.

#589 GENETIC CONSEQUENCES OF WORK AT LEAD MINE AND DYE-HOUSES. Pour-Jafari, H, Pour-Jafari, B. Hamadan University of Medical Sciences, Hamadan, Hamadan, Iran.

#590 FOOD COMPOSITION AS A CONFOUNDING FACTOR IN THE COMET ASSAY IN VIVO. Prá, D¹, Franke, SIR¹, Yoneama, ML², Giulian, R³, Dias, JF³, Erdtmann, B⁴, Henriques, JAP⁵. ¹PPG em Nutrição Clínica/Curso de Nutrição/DEDFIS UNISC; PPGBCM/PPGBM – UFRGS, Santa Cruz do Sul; Porto Alegre, RS, Brazil, ²UNISINOS, São Leopoldo, RS, Brazil, ³Instituto de Física - UFRGS, Porto Alegre, RS, Brazil, ⁴Centro de Biotecnologia - UCS, Caxias do Sul, RS, Brazil, ⁵Curso de Farmácia - ULBRA, Canoas, RS, Brazil.

#591 DOSE-RESPONSE COMPARISON OF MICRONUCLEATED RETICULOCYTE FREQUENCIES IN MOUSE PERIPHERAL BLOOD WITH FOUR GENOTOXIC AGENTS BY FLOW CYTOMETRY AND SLIDE-BASED ENUMERATION. Recio, L¹, Caspary, B², Kissling, G³, Torous, D⁴, Witt, KL². ¹ILS, Inc, Research Triangle Park, NC, United States, ²Environmental Toxicology Program NIEHS, Research Triangle Park, NC, United States, ³Biostatistics Branch NIEHS, Research Triangle Park, NC, United States, ⁴Litron Laboratories, Rochester, NY, United States.

#592 ASSESSMENT OF GENE EXPRESSIONS FOR ENVIRONMENTAL CONTAMINANTS TOXICITY MONITORING WITH THE NEMATODE Caenorhabditis elegans. Roh, J, Park, S, Choi, J. University of Seoul, Faculty of Environmental Engineering, Seoul, South Korea.

#593 DOWN-REGULATION OF SELENOPROTEIN W AS MOLECULAR TARGET OF METHYLMERCURY IN HUMAN NEURONAL CELLS IS ROS INDEPENDENT AND GSH DEPLETION DEPENDENT. Ryu, JC, Kim, YJ, Jeon, HK, Kim, MJ. Korea Institute of Science & Technology, Seoul, South Korea.

#594 GENOTOXICITY OF DIPHENYL DISELENIDE IN CHINESE LUNG HAMSTER FIBROBLAST. Saffi, J¹, Saffi, J², Rosa, RM¹, Hoch, N¹, Santos, RS¹, Moura, DJ¹, Picada, JN¹, Henriques, JAP¹, Henriques, JAP². ¹Biophisics Department– Federal University of Rio Grande do Sul, Porto Alegre, S, Brazil, ²Lutheran University of Brazil, Canoas, RS, Brazil.

#595 CELLULAR RESPONSES TO THE GENOTOXIC STRESS INDUCED BY CISPLATIN AND TEMOZOLOMIDE IN ASTROCYTIC CELL LINE (U343 MG-A). Bahia, MO¹, Carminati, PO², Carlotti, CG³, Sakamoto-Hojo, ET⁴. ¹Departamento de Patologia, Universidade Federal do Pará, Pará, SP, Brazil, ²Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil, ³Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil, ⁴Departamento de Biologia, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, USP, Ribeirao Preto, SP, Brazil.

#596 GENOTOXICITY OF AMPHETAMINES. Salazar, AM, Miranda, E, Sordo, M, Ostrosky-Wegman, P. Universidad Nacional Autonoma de Mexico (UNAM), Mexico, DF, Mexico.

#597 ABNORMAL EXPRESSION OF Bcl-2 AND Bax DURING RAT TONGUE CARCINOGENESIS INDUCED BY 4-NITROQUINOLINE 1-OXIDE. Ribeiro, DA, Salvadori, DMF, Marques, MEA. UNESP, Botucatu, SP, Brazil.

#598 POLYMORPHIC METABOLIZING GENES AND SUSCEPTIBILITY TO CORONARY ARTERY DISEASE. Bazo, AP, de Camargo, EA, da Silva, GN, Salvadori Jr., D, Salvadori, RAF, Ribeiro, LR, de Camargo, JLV, Salvadori, DMF. UNESP, Botucatu, SP, Brazil.

#599 INVESTIGATING THE MUTAGENIC EFFECTS OF THE TOBACCO-DERIVED NITROSAMINE, NNK, IN Msh6-DEFICIENT MICE. Sandercock, LE, Hahn, JN, Giesbrecht, JL, Jirik, FR. University of Calgary, Calgary, Alberta, Canada.

#600 DNA LESIONS AND RESPONSE TO INDUCED DNA DAMAGE IN LYMPHOCYTES OF BREAST CANCER PATIENTS. Santos, RA¹, Carrara, HHA³, Andrade, JM³, Takahashi, CS². ¹Departamento de Genética da Faculdade de Medicina de Ribeirão Preto- USP, Ribeirão Preto, SP, Brazil, ²Departamento de Biologia da Faculdade de Filosofia Ciências de Letras de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil, ³Departamento de Ginecologia e Obstetrícia da Faculdade de Medicina de Ribeirão Preto-USP, Ribeirão Preto, SP, Brazil.

#601 DIESEL EXHAUST PARTICLES CAUSE INCREASED LEVELS OF DNA DELETIONS AND DNA ADDUCT FORMATION AFTER TRANSPLACENTAL EXPOSURE IN MICE. Reliene, R¹, Hlavacova, A¹, Mahadevan, B², Baird, WM², Schiestl, RH¹. ¹UCLA, Los Angeles, CA, United States, ²Oregon State University, Corvallis, OR, United States.

#602 INHIBITORY EFFECTS OF THE POLYCHLORINATED PESTICIDE CHLORDANE AND CONSTITUENT COMPOUNDS/METABOLITES ON HUMAN CYTOCHROME P450 ISOZYME ACTIVITY IN VITRO. Schrader, TJ, Langlois, I. Toxicology Research Division, Health Canada, Ottawa, Ontario, Canada.

#603 COMPARISON OF RAT PERIPHERAL BLOOD AND BONE MARROW ERYTHROCYTE MICRONUCLEUS FREQUENCY USING FLOW CYTOMETRIC ANALYSIS. Engel, ME, Weiner, SK, Schuler, MJ. PGRD Groton Laboratories, Pfizer Inc., Groton, CT, United States.

#604 DIFFERENTIAL GENE EXPRESSION IN RAT KIDNEYS: INFLUENCE OF STRAIN, SEX, AND DIET. Seidel, SD, Hung, S-C, Kan, HL, Gollapudi, BB. Toxicology and Environmental Research & Consulting, The Dow Chemical Company, Midland, MI, United States.

#605 IN VITRO GENOTOXICITY OF PAH MIXTURES AND ORGANIC EXTRACT FROM URBAN AIR PARTICLES. Sevastyanova, O¹, Binkova, B¹, Topinka, J¹, Kalina, I², Popov, T³, Suchankova, Z¹, Farmer, PB⁴, Sram, RJ¹. ¹Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Prague, Czech Republic, ²University PJ Safarik, Kosice, Slovakia, ³National Center of Hygiene, Sofia, Bulgaria, ⁴Biocentre, University of Leicester, Leicester, United Kingdom.

#606 EVALUATION OF RADIATION INDUCED DNA DAMAGE AND DNA REPAIR IN PROSTATE AND BREAST CANCER PATIENTS USING ALKALINE AND NEUTRAL COMET ASSAY. Shahidi, M¹, Mozdarani, H². ¹Mazandaran University of Medical Sciences, Sari, Iran, ²Tarbiat Modarres University, Tehran, Iran.

#607 BIMOLANE INDUCES MULTIPLE TYPES OF CHROMOSOMAL ABERRATIONS IN HUMAN LYMPHOCYTES IN VITRO: A CONVENTIONAL AND MOLECULAR CYTOGENETIC STUDY. Shambhu Kumar, R¹, Shambhu Kumar, R², Eastmond, DA¹. ¹University of California, Riverside, Riverside, CA, United States, ²SITEK Research Laboratories, Rockville, MD, United States.

#608 GLOBAL GENE EXPRESSION, DNA DAMAGE, AND INHIBITION OF SPONTANEOUS MUTAGENESIS AT HPRT BY VANILLIN AND CINNAMALDEHYDE. Shaughnessy, DT¹, King, A², Ducharme, DM-K³, DeMarini, DM⁴, Klein, CB². ¹Laboratory of Molecular Carcinogenesis, NIEHS, Research Triangle Park, NC, United States, ²New York University School of Medicine, Nelson Institute of Environmental Medicine, Tuxedo, NY, United States, ³Microarray Group, NIEHS, Research Triangle Park, NC, United States, ⁴Environmental Carcinogenesis Division, US Environmental Protection Agency, Research Triangle Park, NC, United States.

#609 PREDICTING MUTAGENICITY IN THE AMES TEST USING A BATTERY OF SAR SYSTEMS. Sjögren, M, Carlsson, L, Boyer, S, Bolcsfoldi, G. Safety Assessment, AstraZeneca R&D, Stockholm, Sweden.

#610 MICROARRAY ANALYSIS OF MONONUCLEAR CELL GENE EXPRESSION IN WORKERS EXPOSED TO BENZENE. Zhang, L¹, Lan, Q², Li, G³, Hubbard, AE¹, Forrest, MS¹, McHale, CM¹, Vermeulen, R², Zhao, X¹, Shen, M², Yin, S³, Smith, MT¹, Rothman, N². ¹School of Public Health, University of California, Berkeley, CA, United States, ²Division of Cancer Epidemiology and Genetics, NCI, Bethesda, MD, United States, ³Chinese Center for Disease Control and Prevention, Beijing, China.

#611 TOXICOLOGICAL RESPONSES OF HUMAN CELLS TO LOW DOSE AS - IMPLICATIONS FOR RISK ASSESSMENT. Snow, ET. Deakin University, Burwood, Victoria, Australia.

#612 GENETIC POLYMORPHISMS OF PON1 AND THEIR ROLE ON THE SUSCEPTIBILITY TO DNA DAMAGE. Rojas, E¹, Quintanilla, B¹, Solis, M¹, Sordo, M², Ostrosky, P². ¹Sección Externa de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, Mexico, ²Instituto de Investigaciones Biomédicas de la UNAM, México City, Mexico.

#613 A CONTAMINATED FISH DIET AND GERMLINE MUTATIONS AT REPETITIVE DNA LOCI IN LAB MICE. Somers, CM¹, Somers, CM², Valdes, EV³, Kjoss, VA¹, Vallaincourt, AM⁴, Quinn, JS¹. ¹McMaster University, Department of Biology, Hamilton, ON, Canada, ²The University of Regina, Department of Biology, Regina, SK, Canada, ³Nutrition Centre, Metropolitan Toronto Zoo, Scarborough, ON, Canada, ⁴Ontario Ministry of Natural Resources, Sport Fish Monitoring Program, Etobicoke, ON, Canada.

#614 GENERAL ENHANCEMENT OF MUTAGENIC POTENCY OF VARIOUS MUTAGENS DUE TO DELETED GENES IN THE ?uvrB STRAINS TA98 AND TA100 OF SALMONELLA COMPARED WITH STRAINS CONTAINING ONLY A POINT MUTATION IN uvrB. Swartz, CD¹, Parks, N², Schaaper, RM³, DeMarini, DM². ¹University of North Carolina, Chapel Hill, NC, United States, ²Environmental Carcinogenesis Division, USEPA, Research Triangle Park, NC, United States, ³Laboratory of Molecular Carcinogenesis, NIEHS, Research Triangle Park, NC, United States.

#615 PROTECTIVE EFFECT OF THE PROPOLIS AGAINST CHROMOSOMAL DAMAGE INDUCED BY DOXORUBICIN IN VIVO AND IN VITRO. Tavares, DC¹, Tonin, CCC¹, Barcelos, GRM¹, Silva, LF¹, Takahashi, CS², Bastos, JK³. ¹Universidade de Franca, Franca, SP, Brazil, ²FFCLRP-Universidade de São Paulo, Ribeirão Preto, SP, Brazil, ³FCFRP-Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

#616 BIOMARKERS OF AIR POLLUTION EXPOSURE-STUDY IN POLICEMEN IN PRAGUE. Topinka, J, Binkova, B, Sevastyanova, O, Chvatalova, I, Milcova, A, Lnenickova, Z, Suchankova, Z, Solansky, I, Sram, RJ. Institute of Experimental Medicine AS CR and Health Institute of Central Bohemia, Prague, Czech Republic.

#617 GENETIC POLYMORPHISMS AND ENVIRONMENTAL FACTORS INVOLVED IN GASTRIC CANCER IN TWO COLOMBIAN POPULATIONS. Torres, MM¹, Sicard, DM¹, Silva, IZ¹, Salej, J², Groot, H¹. ¹Universidad de los Andes, Bogotá, Colombia, ²Hospital Militar Central, Bogotá, Colombia.

#618 APPLICATION OF THE ALKALINE COMET ASSAY IN MEASURING DNA REPAIR CAPACITY IN HUMAN POPULATIONS. Trzeciak, AR¹, Barnes, J¹, Ejiogu, N³, Foster, K³, Zonderman, AB², Evans, MK¹. ¹DNA Repair Unit, Laboratory of Cellular and Molecular Biology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States, ²Cognition Section, Laboratory of Personality and Cognition, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States, ³Health Disparities Research Section, Clinical Research Branch, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States.

#619 THE FLEMISH HEALTH AND ENVIRONMENT BIOMONITORING PROGRAM: ASSOCIATIONS BETWEEN EXPOSURE OF NEONATES MEASURED IN CORD BLOOD AND CLINICAL PARAMETERS AT BIRTH. Bruckers, L¹, Neelen, V², Koppen, G³, Bilau, M⁴, Van De Mieroop, E², Brits, E³, Goeyens, K⁵, Covaci, A⁶, Baeyens, W⁵, Schoeters, G³, van Larebeke, N⁴. ¹Limburgs Universitair Centrum, Diepenbeek, Belgium, ²Provincial Institute for Hygiene, Antwerp, Belgium, ³Flemish Institute of Technological Research, Mol, Belgium, ⁴Ghent University, Ghent, Belgium, ⁵Vrije Universiteit Brussel, Brussel, Belgium, ⁶University of Antwerp, Antwerp, Belgium.

#620 A MODIFIED COMET-ASSAY TO ASSESS NUCLEOTIDE EXCISION REPAIR. Langie, SAS, Godschalk, RWL, Knaapen, AM, Brauers, KJJ, Van Schooten, FJ. Maastricht University, Maastricht, Netherlands.

#621 NUTRACEUTICALS WITH ANTI-SKIN TUMOR ACTIVITY. Villaseñor, IM, Bravo, NFC, Ortega, KJL, Simon, MKB, Villanueva, AMA. Institute of Chemistry, University of the Philippines, Diliman, Quezon City, Philippines.

#622 SEASONAL GENOTOXICITY BIOMONITORING OF THE GUAÍBA LAKE BASIN USING THE GOLDEN MUSSEL AS SENTINEL ORGANISM. Villela, IV¹, Oliveira, IM¹, Silveira, J¹, Silva, J², Henriques, JAP². ¹Centro de Biotecnologia Universidade federal do Rio Grande do Sul, Porto Alegre, RS, Brazil, ²Curso de Biologia e Farmácia Universidade Luterana do Brasil, Canoas, RS, Brazil.

#623 MAMMALIAN CELL CYTOTOXICITY AND GENOTOXICITY OF NEW DRINKING WATER DISINFECTION BY-PRODUCTS. Wagner, ED¹, Muellner, MG¹, Richardson, SD², Plewa, MJ¹. ¹University of Illinois at Urbana-Champaign, Urbana, IL, United States, ²U.S. Environmental Protection Agency, Athens, GA, United States.

#624 SOMATIC MACROINDELS OCCUR FREQUENTLY IN YOUNG MICE. Wang, J¹, Gonzalez, K¹, Hill, K², Tsai, B¹, Scaringe, W¹, Sommer, S¹. ¹City of Hope/Beckman Research Institute, Duarte, CA, United States, ²University of Western Ontario, London, ON Canada, Canada.

#625 MUTATIONAL AND TRANSCRIPTIONAL RESPONSES OF STATIONARY- AND LOG-PHASE SALMONELLA TO MX: CORRELATION OF MUTATIONAL RESPONSE TO CHANGES IN GENE EXPRESSION. Ward, WO¹, Swartz, C¹, Porwollik, S², Hanley, NM¹, Warren, SH¹, McClelland, M², DeMarini, DM¹. ¹EPA, Research Triangle Park,NC, United States, ²Sidney Kimmel Cancer Center, SanDiego, CA, United States.

#626 PHOTOMUTAGENIC PROPERTY OF A FLAVORING MALTOL WITH UVA-IRRADIATION IN BACTERIAL CELLS. Watanabe-Akanuma, M¹, Ohta, T². ¹Kureha Chemical Industry, Tokyo, Japan, ²Tokyo Univ. of Pharmacy and Life Science, Hachioji, Tokyo, Japan.

#627 DNA-DAMAGE REDUCING EFFECTS OF QUERCETIN AND QUERCETIN-RICH FRUIT JUICE IN HUMAN LYMPHOCYTES. Wilms, LC, Kleinjans, JCS. Maastricht University, Maastricht, Netherlands.

#628 MUTAGENESIS INDUCED BY BENZO[a]PYRENE WAS AGE-DEPENDENT IN MURINE SPERMATOGENIC CELLS AND GENERALLY LOWER THAN FOR LIVER. Xu, GX¹, McMahan, CAM², Walter, CAW¹. ¹Department of Cellular & Structural Biology, The Univeristy of Texas Health Science Center at San Antonio, San Antonio, TX, United States, ²Department of Pathology, The Univeristy of Texas Health Science Center at San Antonio, San Antonio, TX, United States.

#629 THE EFFECT OF (-)-EPIGALLOCATECHIN GALLATE ON REACTIVE OXYGEN SPECIES INDUCED GENOTOXICITY. Yu, T-WY, Dashwood, RD. Oregon State University, Corvallis, OR, United States.

#630 ANTI-GENOTOXIC EFFECT OF Aloysia triphylla INFUSION AGAINST ACRYLAMIDE-INDUCED DNA DAMAGE AS SHOWN BY THE COMET ASSAY TECHNIQUE. Zamorano-Ponce, E¹, Morales, C¹, Ramos, D¹, Sepúlveda, C¹, Cares, S¹, Rivera, P¹, Fernández, J¹, Carballo, MA². ¹Laboratorio de Genética Toxicológica (GENETOX), Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad del Bío-Bío, Chillán, Chile, ²Citogenética y Genética Toxicológica, CIGETOX, Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina.

#631 THE EFFECT OF SATURATED AND UNSATURATED FATTY ACIDS ON GENOTOXIC ACTIVITY OF FOOD MUTAGEN 2-AMINO-3-METHYLIMIDAZO[4,5-F]QUINOLINE (IQ). Zegura, B¹, Duh, T², Kac, J², Mlinaric, A², Filipic, M¹. ¹Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Ljubljana, Slovenia, ²Faculty of Pharmacy, Department of Pharmaceutical Biology, University of Ljubljana, Ljubljana, Slovenia.

#632 INHIBITORY EFFECT OF GRAPE PROCYANIDINS ON ETHANOL-INDUCED ABNORMAL EXPRESSION OF APOPTOSIS-RELATING GENES IN RAT HEPATOCYTES. Zhong, JY, Duan, XY, Zhang, LH. Nutrition Institute, Medical College of Qingdao University, Qingdao, Shandong, China.

LB24 INDUSTRIAL EMISSIONS AND HEALTH HAZARDS AMONG SELECTED INDUSTRY WORKERS AT ELEME, NIGERIA. Ana, Gree, Sridhar, MKC. Department of Environmental Health Sciences, Faculty of Public Health, College of Medicine, Ibadan, Nigeria.

LB25 ROLE OF DNA DAMAGE CHECKPOINTS Chk1 AND Chk2 IN BRCA2-Rad51 INTERACTION. Bahassi, EM, Penner, G, Stambrook, P. Department of Cell Biology, Neurobiology and Anatomy; University of Cincinnati, Cincinnati, OH, United States.

LB26 CHANGES IN FISHER RAT HEPATIC TRANSCRIPTOME DUE TO DIETARY EXPOSURE TO AROCLOR 1254. Basford, TM, Means, JC. Department of Chemistry and Great Lakes Environmental and Molecular Science Center, Western Michigan University, Kalamazoo, MI, Unites States.

LB27 GENOTOXICITY OF SICK HOUSE SYNDROME AND ATOPIC DERMATITIS PATIENTS. Lee, E^1,2,3, Oh, E², Lee, J^2,3, Park, S^2,3, Hong, E^2,3. ¹Department of Preventive Medicine, School of Medicine, Korea University, Seoul, South Korea, ²Medical Research Center for Toxico-Genomics and Proteomics, School of Medicine, Korea University, Seoul, South Korea, ³Department of Public Health, School of Medicine, South Korea University, Seoul, South Korea.

LB28 INCREASED DNA DAMAGE OF LYMPHOCYTES AND SPERM IN WASTE INCINERATION WORKERS. Oh, E¹, Lee, E^1,2,3, Kim, Y¹, Lee, J^1,3, Lim, Y¹, Sul, D^1,4. ¹Medical Research Center for Toxico-Genomics and Proteomics, , School of Medicine, Korea University, Seoul, Korea, ²Department of Preventive Medicine, Medicine, School of Medicine, Korea University, Seoul, South Korea, ³Department of Public Health, School of Medicine, Korea University, Seoul, South Korea, ⁴Graduate School, Korea University, Seoul, South Korea.

LB29 ASSESSMENT OF ALKYL PHENOLS-INDUCED DNA DAMAGE BY COMET ASSAY AND QSAR ANALYSIS. Liu, Z. Chinese Research Academy of Environmental Sciences, Beiyuan Andingmenwai, Beijing, China.

LB30 RESEARCH ON CHROMOSOMAL ABERRATIONS IN HUMAN SPERM AND LYMPHOCYTES EXPOSED TO LARGE-DOSE ?-RAY. Lu, Y, Baohua, Fu, Yuhao, Chen. Department of Toxicology, Henan Institute of Occupational Medicine, Zhengzhou, P.R.China.

LB31 WITHDRAWN

LB32 MICRONUCLEUS FREQUENCY IN CHILDREN ENVIRONMENTALLY EXPOSED TO LEAD. Moreno-Godínez, ME^1,2, Florencio-Rios, A¹, Illades-Aguiar, B¹, Acosta-Saavedra, LC², Cebrián, M², Calderón-Aranda, ES², Sordo, M³, Ostrosky –Wegman, P³. ¹Facultad de Ciencias Químico-Biológicas, Universidad Autonóma de Guerrero, Chilpancingo, Guerrero, México, ²Toxicología, Centro de investigación y de Estudios Avanzados, México. DF, ³Instituto de Investigaciones Biomédicas, Universidad Nacional Autonóma de México, México, DF.

LB33 WITHDRAWN

LB34 BASIC RESEARCH RESULTS THAT DO NOT SUPPORT THE BEIR VII REPORT CONCLUSION REGARDING THE LINEAR-NO-THRESHOLD RISK HYPOTHESIS. Scott, BR, Haque, M, Di Palma, J. Lovelace Respiratory Research Institute, Albuquerque, NM, United States.

LB35 EFFECTS OF LOW-DOSE ?-IRRADIATION ON NORMAL FIBROBLASTS AND KERATINOCYTES FROM CERVIX CANCER PATIENTS. Slonina, D¹, Biesaga, B¹, Urbanski, K², Kojs, Z²; Waligorski, M³. ¹Dept. of Radiation Biology, Centre of Oncology, Kraków, Poland, ²Dept. of Gynaecological Oncology, Centre of Oncology, Kraków, Poland ³Dept. of Medical Physics, Centre of Oncology, Kraków, Poland

LB36 METABOLISM OF THE FOOD ASSOCIATED CARCINOGEN 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE (PhIP) BY HUMAN INTESTINAL MICROBIOTA. Vanhaecke, L¹, Van Hoof, N², Verstraete, W¹. ¹Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Ghent, Belgium. ²Department of Veterinary Public Health and Food Safety, Ghent University, Ghent, Belgium.

LB37 GENOTOXICITY OF N-NITROSO PROPOXUR TO HUMAN GASTRIC CELL IN CULTURE. Wang, TC. Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan.

LB38 INTER-LABORATORY VALIDATION OF THE GENTRONIX GREENSCREEN GC ASSAY: RESULTS FROM 66 REFERENCE COMPOUNDS. Roberts, KJ¹, Hastwell, PW¹, Harvey, JS¹, Billinton, N², Walmsley, RM², Rees, RW¹. ¹ GlaxoSmithKline Research and Development, Ware, Hertfordshire, United Kingdom, ² Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.

LB39 GENOMIC INSTABILITY INDUCED BY LOW DOSES OF GAMMA RADIATION IN TK6 HUMAN LYMPHOBLASTS. Gibbons, CF^1,2, Kadhim, MA², Grosovsky, AJ¹. ¹University of California, Riverside, CA, United States, ²Radiation and Genome Stability Unit, Medical Research Council, Harwell, Oxfordshire, United Kingdom.

LB45 CONSUMPTION OF BRUSSELS SPROUTS PROTECTS PERIPHERAL HUMAN LYMPHOCYTES AGAINST 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-b]-PYRIDIN. Hoelzl, C¹, Glatt, H², Kollok, R², Haidinger, G¹, Kundi, M³, Chakraborty, A¹, Bichler, J¹, Ferk, F¹, Knasmueller, S¹. ¹Institute of Cancer Research, Medical University of Vienna, Vienna, Austria, ²Department of Toxicology, German Institute of Human Nutrition (DIfE), Pottsdam-Rehbruecke, Germany, ³Institute of Environmental Hygiene, University of Vienna, Vienna, Austria.

LB46 TOWARD THE CHARACTERIZATION OF THE MOLECULAR SIGNATURE AND MECHANISM OF ACTION OF THE ANTICANCER DRUGS BY GENE PROFILING. Le Fhvre, A-C^1,2, Boitier, E¹, Marchandeau, J-P¹, Sarasin, A², Thybaud, V¹. ¹Sanofi aventis, Drug Safety Evaluation, Vitry-Sur-Seine Cedex, France, ²Laboratory of Genetic Instability and Cancer, Institut Gustave Roussy, Villejuif, France.

Tuesday, September 6, 2005
3:00 PM–5:00 PM
Seacliff A/B

EMERGING ISSUES SYMPOSIUM—RISK ASSESSMENT

COMPUTATIONAL TOXICOLOGY: AN ALTERNATIVE TO ANIMALS

Chairs: R. Daniel Benz, US FDA, Rockville, MD, United States and
Philip N. Judson, Lhasa, University of Leeds, Leeds, United Kingdom

3:00 PM #633 AN INTRODUCTION TO COMPUTATIONAL TOXICOLOGY

R. Daniel Benz, US FDA, Rockville, MD, United States

3:20 PM #634 PREDICTIVE TOXICOLOGY METHODOLOGY AND PERFORMANCE

Edwin J. Matthews, US FDA, Rockville, MD, United States

3:40 PM #635 PREDICTING PHARMACEUTICAL EFFICACY USING 3-D DESCRIPTORS

Josep R. Prous, Prous Science, Barcelona, Spain

4:00 PM #636 USE OF SAR/QSAR IN EUROPE

Philip N. Judson, Lhasa, University of Leeds, Leeds, United Kingdom

4:15 PM #637 MC4PC PREDICTION OF THE GENETIC TOXICITY POTENTIAL OF ORGANIC MOLECULES

Gilles Klopman, MultiCASE, Beachwood, OH, United States

4:30 PM #638 PREDICTING MAXIMUM RECOMMENDED DAILY DOSE

Naomi Kruhlak, US FDA, Rockville, MD, United States

4:45 PM #639 PREDICTING ABSORPTION, DISTRIBUTION, METABOLISM AND EXCRETION (ADME)

Michael B. Bolger, SimulationsPlus, Lancaster, CA, United States

Tuesday, September 6, 2005
3:00 PM–5:00 PM
Grand Ballroom A

EMERGING ISSUES SYMPOSIUM—MUTATIONAL MECHANISMS

DNA DAMAGE AND MUTATIONAL SPECIFICITY

Chairs: Kathleen A. Hill, University Western Ontario, ON, Canada and
Gerald P. Holmquist, City of Hope, Duarte, CA, United States

3:00 PM #640 NUCLEOTIDE EXCISION REPAIR RATES OF R-BAND, G-BAND AND C-BAND DNA IN MAMMALIAN CELLS

Gerald P. Holmquist, City of Hope, Duarte, CA, United States

3:20 PM #641 ISSUES IN MUTAGENESIS EMERGING FROM IN VIVO ANALYSIS OF GERMLINE AND SOMATIC SPONTANEOUS MUTATIONS IN HUMAN AND MOUSE

Kathleen A. Hill, University of Western Ontario, ON, Canada

3:40 PM #642 DNA ADDUCTS, MUTANT FREQUENCIES, TYPES OF MUTATIONS AND MICROARRAY GENE EXPRESSION PROFILES INDUCED BY ARISTOLOCHIC ACID IN KIDNEY AND LIVER OF BIG BLUE TRANSGENIC RATS

Nan Mei, National Center for Toxicological Research, US FDA, Jefferson, AR, United States

4:00 PM #643 TRACING NUCLEOSIDE INCORPORATION AND ESTROGEN-DEPENDANT DNA OXIDATION IN MCF-7 BREAST CANCER CELLS USING ACCELERATOR MASS SPECTROMETRY

Paul T. Henderson, Lawrence Livermore National Laboratory, Livermore Livermore, CA, United States

4:15 PM #644 DNA LESIONS INDUCED BY ULTRAVIOLET A AND B RADIATION IN HUMAN CELLS: COMPARATIVE ANALYSIS IN THE OVERALL GENOME AND THE P53 TUMOR SUPPRESSOR GENE

Ahmad Besaratinia, City of Hope, Duarate, CA, United States

4:30 PM #645 DYNAMIC MITOCHONDRIAL DNA RESPONSES TO OXIDATIVE DAMAGE IN HUMAN CANCER CELLS

Junjian Z. Chen, McGill University Health Center, Montreal, Quebec, Canada

4:45 PM #646 INHIBITION EFFECTS OF GREEN VEGETABLE OR FRUITS AGAINST 8-OH-dG INDUCED BY NITROPHENANTHRENE DERIVATIVES IN VITRO AND IN VIVO

Nobuyuki Sera, Fukuoka Institute of Health and Environmental Sciences, Fukuoka, Japan

Tuesday, September 6, 2005
3:00 PM–5:00 PM
Seacliff C/D

EMERGING ISSUES SYMPOSIUM—DNA REPAIR

ENDOGENOUS DNA DAMAGE: COMPARISON WITH ENVIRONMENTAL GENOTOXICANTS

Chairs: P.J. Brooks, NIH, Bethesda, MD, United States and
David H. Phillips, Institute of Cancer Research, Sutton, United Kingdom

3:00 PM #647 WHAT MEASURING DNA DAMAGE DOES AND DOES NOT TELL US ABOUT CAUSES OF CANCER AND MUTATION

David H. Phillips, Institute of Cancer Research, Sutton, United Kingdom

3:20 PM #648 TOXICOGENOMICS OF ENDEMIC NEPHROPATHY, AN ENVIRONMENTAL DISEASE

Arthur P. Grollman, SUNY Stony Brook, Stony Brook, NY, United States

3:40 PM #649 BIOLOGICAL EFFECTS OF 8, 5’- CYCLOPURINES: A UNIQUELY TOXIC FORM FOR ENDOGENOUS OXIDATIVE DNA DAMAGE

P.J. Brooks, NIAAA, NIH, Bethesda, MD, United States

4:00 PM #650 MEASUREMENT OF IN VIVO OXIDATIVE DNA DAMAGE BY LIQUID CHROMOTOGRAPHY/MASS SPECTOMETRY

Miral Dizdaroglu, National Institute of Standards and Technology, Gaithersburg, MD, United States

4:15 PM #651 GENOTOXICITY OF ARSENIC AND CADMIUM: OXIDATIVE DNA DAMAGE, DNA REPAIR, ZINC FINGER PROTEINS AND P53

Tanja Schwerdtle, Technical University of Berlin, Berlin, Germany

4:30 PM #652 SPONTANEOUS DNA DAMAGE DUE TO MITOCHONDRIAL METABOLISM TRIGGERS CHECKPOINT ACTIVATION IN REPAIR DEFICIENT Saccharomyces cerevisiae

Wolfram Siede, University of North Texas Health Science Center, Fort Worth, TX, United States

4:45 PM #653 GENOME INSTABILITY CAUSED BY ENDOGENOUS NUCLEOTIDE LESIONS

Tatsuo Nunoshiba, Tohoku University, Sendai, Japan

Tuesday, September 6, 2005
3:00 PM–5:00 PM
Bayview

EMERGING ISSUES SYMPOSIUM—MUTATIONAL MECHANISMS

GENE EXPRESSION AND GENOMIC CHANGES

Chairs: Cynthia A. Afshari, Amgen, Thousand Oaks, CA, United States and Keiji Wakabayashi, Cancer Prevention Basic Research Project, Kyoto, Japan

3:00 PM #654 CAN THE APPLICATION OF GENOMICS IMPACT THE DISCIPLINE OF GENETIC TOXICOLOGY?

Cinthia A. Afshari, Amgen, Inc., Thousand Oaks, CA, United States

3:20 PM #655 ENDOGENOUS AND EXOGENOUS ORIGINS OF ABERRANT DNA METHYLATION

Toshikazu Ushijima, National Cancer Center Research Institute, Tokyo, Japan

3:40 PM #656 GENE ALTERATIONS AND CHANGES OF GENE EXPRESSION IN COLON CARCINOGENESIS OF ANIMALS

Keiji Wakabayashi, Cancer Prevention Basic Research Project, Kyoto, Japan

4:00 PM #657 PRELIMINARY VALIDATION OF A HIGH-THROUGHPUT MAMMALIAN IN VITRO GENOTOXICITY SCREENING ASSAY: GREENSCREEN TK

Paul W. Hastwell, GlaxoSmithKline, Hertfordshire, UK

4:15 PM #658 CELL DIFFENTIATION AND DOMINANT SIGNALING PATHWAY SIGNATURES IN THE MOLECULAR CLASSIFICATION OF HUMAN BREAST CANCER CELL LINES

Cindy A. Wilson, University of California Los Angeles, Los Angeles, CA United States

4:30 PM #659 EFFECT OF P53 GENOTYPE ON GENE EXPRESSION PROFILES IN MURINE LIVER

Suzanne M. Morris, FDA/NCTR, Jefferson, AR, United States

4:45 PM #660 PhIP-INDUCED GLOBAL CHANGES IN GENOME EXPRESSION IN HUMAN PROSTATE CELLS

Chitra F. Manohar, Lawrence Livermore National Laboratory, Livermore, CA, United States

Tuesday, September 6, 2005
3:00 PM–5:00 PM
Grand Ballroom B

EMERGING ISSUES SYMPOSIUM—MUTATIONAL MECHANISMS

NEW TECHNOLOGIES IN MUTATIONAL ANALYSIS FOR GENOMIC AND GENETIC TOXICOLOGY STUDIES

Chairs: Rosalie K. Elespuru, US FDA, Rockville, MD, United States and
Matthew E. Hurles, Wellcome Trust Sanger Institute, Cambridge, United Kingdom

3:00 PM HIGH-THROUGHPUT DNA SEQUENCE ANALYSIS

Daixing Zhou, Solexa Inc., Hayward, CA, United States

3:20 PM #661 NEW ASSAYS FOR GERMLINE MUTATION: IDENTIFYING DE NOVO CHROMOSOMAL REARRANGEMENTS IN SPERM GENOMES

Matthew E. Hurles, Wellcome Trust Sanger Institute, Cambridge, United Kingdom

3:40 PM #662 MEASUREMENT OF SOMATIC MUTATION LOAD IN HUMAN SOLID TISSUES

Steve S. Sommer, City of Hope, Duarte, CA, United States

4:00 PM #664 STATISTICAL ISSUES ASSOCIATED WITH MICROARRAY-BASED GENOME PROFILING

Ru Fang Yeh, University of California, San Francisco, CA, United States

4:15 PM #665 GENOTYPING SELECTION:
MONITORING P53 MUTATIONS DURING TUMOR DEVELOPMENT

Barbara L. Parsons, NCTR, Jefferson, AR, United States

4:30 PM SEARCHING FOR CAUSES OF HUMAN GENETIC DISEASE

Rosalie K. Elespuru, US FDA, Rockville, MD, United States

4:45 PM GENE EXPRESSION PROFILING USING HIGH-DENSITY MICROARRAYS

Alexander Kohlmann, Roche Molecular Systems, Pleasanton, CA, United States

Tuesday, September 6, 2005
5:00 PM–5:30 PM
Grand Ballroom Foyer

REFRESHMENT BREAK

Tuesday, September 6, 2005
5:30 PM–6:15 PM
Grand Ballroom

PLENARY LECTURE—JAN HOEIJMAKERS

Introduction

Toshihiro Ohta, Secretary General of the Japanese Environmental Mutagen Society (JEMS), Tokyo University of Pharmacy and Life Science, Tokyo, Japan

CANCER AND AGING

Jan Hoeijmakers, Erasmus University, Rotterdam, Netherlands

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