Monday, September 5, 2005

Monday, September 5, 2005
7:00 AM–6:30 PM
Market Street Foyer

REGISTRATION OPEN

Monday, September 5, 2005
7:00 AM–8:30 AM

MEMBERSHIP AND PROFESSIONAL DEVELOPMENT COMMITTEE

(Garden Room)

IAEMS BUSINESS MEETING, 1ST MEETING

(Seacliff A/B)

GERM CELL MUTAGENESIS SPECIAL INTEREST GROUP

(Seacliff C/D)

Monday, September 5, 2005
8:30 AM–9:15 AM
Grand Ballroom

PLENARY LECTURE—RITA R. COLWELL

Introduction

Helena Groot de Restrepo, Vice President of the Latin American Association of Mutagenesis, Teratogenesis, and Carcinogenesis (ALAMCTA), University of the Andes, Bogota, Colombia

#216 CLIMATE AND HUMAN HEALTH: ENVIRONMENTALLY MEDIATED INFECTIOUS DISEASES

Rita R. Colwell, University of Maryland, College Park, MD, United States

Monday, September 5, 2005
9:15 AM–10:00 AM
Grand Ballroom

PLENARY LECTURE—LOUIS J. GUILLETTE, JR.

Introduction

Amelia Guevara, President of the Philippine Environmental Mutagen Society (PEMS), University of the Philippines, Quezon City, Philippines

#217 CONTAMINANTS, GENES, AND HEALTH: LESSONS FROM WILDLIFE

Louis J. Guillette, Jr., University of Florida, Gainesville, FL, United States

Monday, September 5, 2005
10:00 AM–10:30 AM
Grand Ballroom Foyer

REFRESHMENT BREAK

Monday, September 5, 2005
10:30 AM–12:30 PM
Grand Ballroom A

CURRENT ISSUES SYMPOSIUM—DNA REPAIR

CELL CYCLE AND HOW CELLS SENSE THEIR ENVIRONMENT

Chairs: Peter J. Stambrook, University of Cincinnati, Cincinnati, OH, United States and
Ronald A. Laskey, MRC Research Centre, Cambridge, United Kingdom

10:30 AM #218 CONTROL OF DNA REPLICATION BY GEMININ AND MCM3 ACETYLASE

Ronald A. Laskey, MRC Research Centre, Cambridge, United Kingdom

11:00 AM #219 DYNAMICS OF DNA DOUBLE-STRAND BREAKS, RECOMBINATION, AND CHROMOSOME TRANSLOCATIONS

Roland Kanaar, Erasmus University, Rotterdam, Netherlands

11:30 AM #220 GENOME INTEGRITY VIA SIGNALING NETWORKS: LESSONS FROM MODEL ORGANISMS

Yolanda Sanchez, University of Cincinnati Medical Center, Cincinnati, OH, United States

11:50 AM #221 CANCER SUSCEPTIBILITY AND BRCA2 FUNCTIONS

Ashok R. Venkitaraman, MRC Research Centre, Cambridge, United Kingdom

12:10 PM #222 PHOSPHOSERINE/THREONINE-BINDING DOMAINS: MOLECULAR INTEGRATORS OF PROTEIN KINASE SIGNALING NETWORKS

Michael B. Yaffe, MIT, Cambridge, MA, United States

Monday, September 5, 2005
10:30 AM–12:30 PM
Seacliff A/B

CURRENT ISSUES SYMPOSIUM—ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

ENVIRONMENTAL CONTAMINANT EXPOSURE AND MUTAGENESIS IN WILDLIFE

Chairs: Christopher M. Somers, University Regina, SK, Canada and
Frederik-Jan van Schooten, Maastricht University, Maastricht, Netherlands

10:30 AM #223 INTEGRATING TOXICOLOGY, MUTAGENESIS, AND POPULATION GENETICS

John W. Bickham, Texas A&M University, College Station, TX, United States

11:00 AM #224 ENVIRONMENTAL RADIATION:
GLOBAL DISTRIBUTION AND GENETIC RISKS TO WILDLIFE

Douglas R. Boreham, McMaster University, Hamilton, ON, Canada

11:30 AM #225 ASSESSING THE POPULATION-LEVEL IMPACTS OF GENOTOXIC SUBSTANCES IN AQUATIC SYSTEMS

Paul A. White, Health Canada, Ottawa, ON, Canada

11:50 AM #226 URBAN WILDLIFE AS SENTINELS FOR MUTAGENESIS: AN UNDER-UTILIZED RESOURCE?

Frederik-Jan van Schooten, Maastricht University, Maastricht, Netherlands

12:10 PM #227 URBAN POLLUTION: DOGS AS SENTINEL ANIMALS FOR NEURODEGENERATION AND NEUROCARCINOGENESIS

Lilian Calderon-Garciduenas, Instituto Nacional de Pediatria, Mexico City, Mexico and Department Biomedical Pharmaceutical Sciences, University of Montana, Missoula, MT, United States

Monday, September 5, 2005
10:30 AM–12:30 PM
Grand Ballroom B

CURRENT ISSUES SYMPOSIUM—DNA REPAIR

INTERACTIVE COMPETITION AMONG DNA REPAIR PATHWAYS

Chairs: Jac A. Nickoloff, University of New Mexico, Albuquerque, NM, United States and
John B. Hays, Oregon State University, Corvallis, OR, United States

10:30 AM #228 DNA MISMATCH REPAIR AND NUCLEOTIDE EXCISION REPAIR RESPONSES TO “MISMATCHED” UV PHOTOPRODUCTS

John B. Hays, Oregon State University, Corvallis, OR, United States

11:00 AM #229 KINASE-INDEPENDENT FUNCTION FOR DNA-DEPENDENT PROTEIN KINASE IN DOUBLE-STRAND BREAK REPAIR BY HOMOLOGOUS RECOMBINATION

Jac Nickoloff, University New Mexico, Albuquerque, NM, United States

11:30 AM #230 FACTORS FROM MULTIPLE REPAIR PATHWAYS PARTICIPATE IN THE UNCOUPLING OF INTERSTRAND CROSS-LINKS

Randy J. Legerski, University of Texas, Houston, TX, United States

11:50 AM #231 A GLOWING REPORT: USE OF FLUORESCENT PROBES TO STUDY THE COORDINATION OF RECOMBINATION AND DNA REPLICATION IN E. coli K-12

Steven J. Sandler, University of Massachusetts, Amherst, MA, United States

12:10 PM #232 THE MANY FACES OF XPG: COORDINATING ROLES IN NUCLEOTIDE AND BASE EXCISION REPAIR AND TRANSCRIPTION-COUPLED REPAIR

Priscilla K. Cooper, Lawrence Berkeley National Laboratory, Berkeley, CA, United States

Monday, September 5, 2005
10:30 AM–12:30 PM
Bayview

CURRENT ISSUES SYMPOSIUM—MUTAGENESIS AND HUMAN DISEASE

MITOCHONDRIAL DNA DAMAGE AND HUMAN DISEASE

Chairs: Susan P. LeDoux, University South Alabama, Mobile, AL, United States and
Yusaku Nakabeppu, Kyushu University, Kyushu, Japan

Sponsored by The Ellison Medical Foundation

10:30 AM MITOCHONDRIA AND APOPTOSIS: PASO DOBLE

Douglas R. Green, La Jolla Institute Allergy and Immunology, San Diego, CA, United States

11:00 AM #233 TWO DISTINCT PATHWAYS FOR CELL DEATH TRIGGERED BY ACCUMULATION OF 8- OXOGUANINE IN NUCLEAR AND MITOCHONDRIAL GENOMES

Yusaku Nakabeppu, Kyushu University, Kyushu, Japan

11:30 AM #234 DISEASES FROM MUTATIONS IN THE GENE FOR THE MITOCHONDRIAL DNA POLYMERASE

William C. Copeland, NIEHS, Research Triangle Park, NC, United States

11:50 AM #235 MITOCHONDRIA, OXIDATIVE STRESS, IRON, AND TELOMERASE: WHAT IS THE CONNECTION?

Bennett Van Houten, NIEHS, Research Triangle Park, NC, United States

12:10 PM #236 ENHANCING MITOCHONDRIAL DNA REPAIR: A NOVEL THERAPEUTIC STRATEGY

Glenn L. Wilson, University of South Alabama, Mobile, AL, United States

Monday, September 5, 2005
10:30 AM–12:30 PM
Seacliff C/D

CURRENT ISSUES SYMPOSIUM—MUTAGENESIS AND HUMAN DISEASE

MOLECULAR EPIDEMIOLOGY OF CHILDREN’S ENVIRONMENTAL HEALTH

Chairs: Nina T. Holland, University of California, Berkeley, United States and
Mathuros Ruchirawat, Chulabhorn Institute, Bangkok, Thailand

10:30 AM #237 ENVIRONMENTAL EXPOSURES AND THE MOLCULAR EPIDEMIOLOGY OF CHILDHOOD LEUKEMIA

Patricia A. Buffler, University of California, Berkeley, CA, United States

11:00 AM #238 CHILDREN’S ENVIRONMENTAL HEALTH IN DEVELOPING COUNTRIES

Mathuros Ruchirawat, Chulabhorn Institute, Bangkok, Thailand

11:30 AM #239 ASSESSING FETAL, INFANT, AND CHILDHOOD EXPOSURES TO ENVIRONMENTAL TOXICANTS

Dana B. Barr, Centers for Disease Control, Atlanta, GA, United States

11:50 AM #240 AFLATOXINS, CHILD GROWTH, AND IMMUNITY IN WEST AFRICA: FROM DISEASE MECHANISMS TO DISEASE PREVENTION

Paul C. Turner, Leeds University, Leeds, United Kingdom

12:10 PM #241 GENETIC AND IMMUNOLOGICAL BIOMARKERS IN MINORITY POPULATIONS

Nina T. Holland, University of California, Berkeley, CA, United States

Monday, September 5, 2005
1:00 PM–3:00 PM
Pacific Concourse

POSTERS ATTENDED AND EXHIBITS OPEN

MECHANISMS OF MUTAGENESIS AND DNA REPAIR

Odd numbered posters will be attended from 1:00 PM–2:00 PM and even numbered posters will be attended from 2:00 PM–3:00 PM.

#242 APPLICATION OF QUANTITATIVE PCR FOR THE DETECTION OF NUCLEAR AND MITOCHONDRIAL DNA DAMAGE INDUCED BY UVA/UVB RADIATION IN Saccharomyces cerevisiae. Acevedo-Torres, K¹, Ruiz, A², Morales, GE², Ayala-Torres, S¹, Matta, J², Torres-Ramos, CA¹. ¹University of Puerto Rico Medical Sciences Campus, San Juan, PR, United States, ²Ponce School of Medicine, Ponce, PR, United States.

#243 EFFECTS OF MSH3 GENE DELETION ON TARGETED HOMOLOGOUS RECOMBINATION IN ERCC1 WILD-TYPE OR ERCC1 KNOCK-OUT CELLS. Adair, GM, Robison, T, Della Coletta, L, Holt, A, Rowley, B, Lowery, M, Maywald, R, Nairn, RS. Univ. of Texas M.D. Anderson Cancer Ctr., Smithville, TX, United States.

#244 ERCC1-XPF: A MULTIFUNCTIONAL DNA REPAIR ENDONUCLEASE. Ahmad, A¹, Robinson, A¹, Kanaar, R², Hoeijmakers, Y², Niedernhofer, L¹. ¹University of Pittsburgh, Pittsburgh, PA, United States, ²Erasmus University, Rotterdam, Netherlands.

#245 THE COMPETITION OF mutS WITH mutT OR recA DEFICIENT STRAINS OF BACTERIA Escherichia coli AND Salmonella enterica SEROVAR Typhimurium DURING PROLONGED STATIONARY PHASE. Bacun-Druzina, V¹, Ratkaj, I¹, Vrbica, M¹, Gjuracic, K², Franekic Colic, J¹. ¹Faculty of Food Technology and Biotechnology, Zagreb, Croatia, ²Pliva, Research Division, Zagreb, Croatia.

#246 ENHANCED POINT MUTATION BUT NOT RECOMBINATION IN THE ABSENCE OF Msh6. Barrera-Oro, J¹, Gorden, E¹, Kucherlapati, R², Shao, C¹, Tischfield, J¹. ¹Rutgers University, Piscataway, NJ, United States, ²Harvard Medical School, Boston, MA, United States.

#246A IMPACT OF FOLIC ACID DEFICIENCY ON GENOME STABILITY IN BRCA1 AND BRCA2 GERMLINE MUTATION CARRIERS. Beetstra S², Salisbury C¹, Turner J¹, Altree M³, McKinnon R², Suthers G³, Fenech M¹. ¹CSIRO Health Sciences and Nutrition, Adelaide, SA, Austrailia, ² University of South Australia, Samson Institute, Adelaide, SA, Austrailia, ³Familial Cancer Unit, SA Clinical Genetics Service, Women’s and Children’s Hospital, North Adelaide, SA, Austrailia.

#247 DNA DAMAGE/REPAIR AND PAPILLOMAVIRUS INFECTION AS RISK FACTORS FOR NONMELANOMA SKIN CANCER. Bendesky, A⁵, Michel, A², Sordo, M¹, Calderon-Aranda, E⁴, Salazar, AM¹, Podoswa, N³, Cebrian, M⁴, Ostrosky-Wegman, P¹. ¹Insituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF, Mexico, ²Hospital General Dr. Manuel Gea Gonzalez, SS, Mexico, DF, Mexico, ³Hospital General Regional No. 1 Gabriel Mancera, IMSS, Mexico, DF, Mexico, ⁴CINVESTAV, Mexico, DF, Mexico, ⁵Facultad de Medicina and Insituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico, Mexico, DF, Mexico.

#248 CHARACTERIZATION OF THE ALDEHYDE REACTIVE PROBE REACTION WITH AP-SITE DNA: INFLUENCE OF AP-LYASE ON ADDUCT STABILITY. Bennett, SE, Lari, SU. Oregon State University, Corvallis,OR, United States.

#249 SIMULATIONS OF HETEROCYCLIC AMINE ACTIVE SITE BINDING IN CYTOCHROME P450 1A2. Bennion, BJ, Lau, EY, Colvin, ME, Felton, JS, Lightstone, FC. Lawrence Livermore National Laboratory, Livermore, CA, United States.

#250 P53 IS REQUIRED FOR THE EFFICIENT GLOBAL GENOMIC REPAIR OF CISPLATIN-INDUCED INTRASTRAND CROSSLINKS. Bhana, S, Hewer, A, Phillips, D, Lloyd, D. ¹University of Kent, Canterbury, Kent, United Kingdom, ²Institute of Cancer Research, Sutton, Surrey, United Kingdom.

#251 IMATINIB (STI571) INHIBITS DNA REPAIR IN BCR/ABL-EXPRESSING CELLS EXPOSED TO ENVIRONMENTAL MUTAGENS. Blasiak, J, Czechowska, A, Arabski, M, Majsterek, I. University of Lodz, Department of Molecular Genetics, Lodz, Poland.

#252 BULKY DNA ADDUCTS AND REPAIR ENZYMES IN EARLY DEVELOPMENT OF ZEBRAFISH. Brooks, E, Atkin, J, Mahadevan, B, Tanguay, RL, Baird, WM. Oregon State University, Corvallis, OR, United States.

#253 TRANSCRIPTION-COUPLED REPAIR OF OXIDATIVE DAMAGE: THE ROLE OF CSB. Budworth, H, Fuss, J, Haltiwanger, BM, Cooper, PK. Lawrence Berkeley National Laboratory, Berkeley, CA, United States.

#254 IMPACT OF PATHOGENIC MUTATIONS IN HUMAN MLH1 ON FORMATION OF THE MUTL? HETERODIMER IN MAMMALIAN CELLS. Buermeyer, AB, Mohd, AB, Palama, B, Ing, B. Oregon State University, Corvallis, OR, United States.

#255 EFFECTS OF Cervi pantotrichum Cornu HERBAL ACUPUNCTURE SOLUTION (CPCHA) ON THE GENETIC TOXICITY. Byun, BH¹, Park, EJ². ¹Dept. of Oriental Medicine, Daegu Haany University, Daegu, South Korea, ²Dept. of Food and Nutrition, Kyungnam University, Masan, South Korea.

#256 A CAUSATIVE ROLE FOR LOSS OF DNA POLYMERASE BETA IN AGING. Cabelof, DC¹, Richardson, A², Matherly, LH¹, Heydari, AR³. ¹Karmanos Cancer Institute, Wayne State University, Detroit, MI, United States, ²University of Texas Health Sciences Center San Antonio, San Antonio, TX, United States, ³Department of Nutrition and Food Science, Wayne State University, Detroit, MI, United States.

#257 RECOGNITION OF DNA ENDS BY THE Mre11 COMPLEX: IMPLICATIONS FOR DOUBLE-STRAND BREAK DETECTION. Cahill, D, Ullman, E, Carney, JP. U. Maryland School of Medicine, Baltimore, MD, United States.

#258 GERMONLINE, A CROSS-SPECIES COMMUNITY ANNOTATION KNOWLEDGEBASE FOCUSSING ON THE GERMLINE, PROVIDES LIFE SCIENTISTS WITH AN ONLINE PUBLISHING TOOL FOR MICROARRAY DATA. Carton, M¹, Niederhauser-Wiederkehr, C¹, Hermida, L¹, van den Broek, S¹, Cherry, M⁴, Yamamoto, M³, Lamb, N², Primig, M¹. ¹Biozentrum & SIB, Basel, Switzerland, ²IGH, Montpellier, Switzerland, ³University of Tokyo, Tokyo, Japan, ⁴Stanford University, Palo Alto, United States.

#259 EFFECT OF CELL CONFLUENCE ON ULTRAVIOLET LIGHT APOPTOTIC RESPONSES IN NUCLEOTIDE EXCISION REPAIR DEFICIENT FIBROBLASTS. Carvalho, H¹, Weinlich, R², Amarante-Mendes, GP², Menck, CFM¹. ¹Depto. de Microbiologia - Instituto de Ciências Biomédicas - Universidade de São Paulo, São Paulo, Brazil, ²Depto. de Imunologia - Instituto de Ciências Biomédicas - Universidade de São Paulo, São Paulo, Brazil.

#260 INFLUENCE OF THE ENVIRONMENTAL EXPOSURE TO PAHs ON SUSCEPTIBILITY TO THE INDUCTION OF DNA DAMAGE. Cebulska-Wasilewska, A¹, Cebulska-Wasilewska, A², Binkova, B³, Sram, RJ³, Kalina, I⁴, Popov, T⁵, Farmer, P⁶. ¹Department of Radiation and Environmental Biology, The H.Niewodniczaski Institute of Nuclear Physics PAN, Radzikowskiego 152, Kraków, Poland, ²Chair of the Epidemiology and Preventive Medicine, Collegium Medicum of Jagiellonian University, Kraków, Poland, ³Institute of Experimental Medicine AS CR and Regional Institute of Health of Central Bohemia, Prague, Czech Republic, ⁴Department of Molecular Biology of the P.J.Šafárik University, Košice, Slovakia, ⁵Department of Toxicology, National Center of Hygiene, Sofia, Bulgaria, ⁶Cancer Biomarkers and Prevention Group, Leicester, United Kingdom.

#261 RIBONUCLEOTIDE REDUCTASE AND E2F IN CELL CYCLE AND DNA DAMAGE RESPONSE IN PLANTS. Lincker, F, Roa, H, Lang, J, Orsini, V, Isac, R, Houlné, G, Chabouté, ME. IBMP/CNRS-ULP, Strasbourg, France.

#262 IONIZING RADIATION AND RESTRICTION ENZYMES INDUCE MICROHOMOLOGY-MEDIATED ILLEGITIMATE RECOMBINATION IN TRANS IN Saccharomyces cerevisiae. Chan, CY, Kiechle, M, Manivasakam, P, Schiestl, RH. Department of Pathology, UCLA, Los Angeles, CA, United States.

#263 THE COMMON A467T MUTATION IN THE CATALYTIC SUBUNIT OF THE HUMAN MITOCHONDRIAL DNA POLYMERASE COMPROMISES CATALYTIC EFFICIENCY AND INTERACTION WITH THE ACCESSORY SUBUNIT. Chan, SSL, Longley, MJ, Copeland, WC. NIEHS/NIH, Research Triangle Park, NC, United States.

#264 DIFFERENTIAL MUTAGENICITY OF GENOTOXIC CARCINOGENS IN NEONATAL AND ADULT MICE. Chen, T¹, Mei, N¹, Slikker III, W², Moore, MM¹, Heflich, RH¹. ¹Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research, US FDA, Jefferson, AR, United States, ²College of Letters and Science, University of California, Los Angeles, CA, United States.

#265 LYSINE 63 POLYUBIQUITINATION PROTECTS CELLS AGAINST BENZO[a]PYRENE-DIOL-EPOXIDE INDUCED MUTATIONS. Chiu, RK, Langie, SAS, Knaapen, AM, Ramaekers, CHMA, Theys, J, Godschalk, RWL, van Schooten, FJ, Lambin, P, Wouters, BG. University of Maastricht, Maastricht, Netherlands.

#266 ADDUCT SIZE LIMITS EFFICIENT AND ERROR-FREE BYPASS ACROSS BULKY N²-GUANINE DNA LESIONS BY HUMAN DNA POLYMERASE ETA. Choi, JY, Guengerich, FP. Vanderbilt University, Nashville, TN, United States.

#267 NEW NUCLEIC ACID BIOSENSORS TO DETECT AND QUANTIFY DNA REPAIR ACTIVITIES BY A FRET ASSAY. Chollat-Namy, A, Gasparutto, D, Cadet, J, Favier, A. CEA Grenoble DRFMC/SCIB/LAN, UNR-E n.3 CEA-UJF, Grenoble, France.

#268 DNA DAMAGE AND REPAIR IN GERM CELLS OF Parp-1^-/- MALE MICE AFTER X-RAY IRRADIATION. Cordelli, E, Fresegna, AM, Viola, C, Pacchierotti, F, Villani, P. ENEA, Rome, Italy, Italy.

#269 PRE-EXPOSURE: MODULATION OF FREQUENCIES AND REPAIR OF DNA DAMAGE? Cramers, P¹, Mullenders, LHF¹, van Zeeland, AA¹, Kleinjans, JCS². ¹Leiden University Medical Center, Leiden, Netherlands, ²Maastricht University, Maastricht, Netherlands.

#270 OXIDATIVE STRESS IN L5178Y MOUSE LYMPHOMA CELLS AND THE IMPACT OF INCUBATION WITH S9 MIXTURE. Czene, S, Johansson, H, Bolcsfoldi, G. AstraZeneca R&D Södertälje, Safety Assessment, Genetic Toxicology, Södertälje, Sweden.

#271 POSITIVE AND NEGATIVE EFFECTS OF DNA DAMAGE FOR MITOTIC RECOMBINATION IN Saccharomyces cerevisiae. Daigaku, Y, Endo, K, Mashiko, S, Yamamoto, K. Graduate School of life Sciences, Tohoku University, Sendai, Japan.

#272 GENERATION AND ANALYSIS OF MOUSE MODELS WITH DEFECTS IN THE Rev1 AND Rev3 TRANSLESION SYNTHESIS POLYMERASES. Jansen, JG, Tsaalbi-Shtylik, A, Pauw, B, de Wind, N. Leiden University Medical Center, Department of Toxicogenetics, Leiden, Netherlands.

#273 DNA DAMAGE INDUCES RAD51-GAMMA-TUBULIN NUCLEAR COMPLEXES IN MAMMALIAN CELLS. Defais, M¹, Lesca, C¹, Germanier, M¹, Roques, C¹, Raynaud-Messina, B², Etievant, C², Wright, M², Monsarrat, B¹, Burlet-Schiltz, O¹. ¹CNRS, IPBS, Toulouse, France, ²CNRS-Pierre Fabre, ISTMT, Toulouse, France.

#274 THE LACK OF EITHER XPC OR CSA LEADS TO IN VIVO ACCUMULATION OF OXIDATIVELY MODIFIED DNA BASES IN HUMAN KERATINOCYTES VIA DIFFERENT MECHANISMS. D’Errico, M¹, Parlanti, E¹, Teson, M², Calcagnile, A¹, Jaruga, P³, Zambruno, G², Stefanini, M⁴, Dizdaroglu, M³, Dogliotti, E¹. ¹National Institute of Health, Rome, Italy, Italy, ²Istituto Dermopatico dell’Immacolata, IRCCS, Rome, Italy, Italy, ³National Institute of Standards and Technology, Gaithersburg, MD, United States, ⁴Consiglio Nazionale delle Ricerche, Pavia, Italy.

#275 TRANSCRIPTIONAL BYPASS OF MODIFIED GUANINE BASES AND UNPAIRED REGIONS OF DNA: BIOCHEMICAL AND MODELING STUDIES. Dimitri, A¹, Burns, JA¹, Broyde, S¹, Geacintov, NE¹, Farley, SA¹, Guengerich, F², Rizzo, C², Goodenough, AK², Scicchitano, DA¹. ¹New York University, New York, NY, United States, ²Vanderbilt University, Nashville, TN, United States.

#276 BENZO(a)PYRENE (BP)-DNA ADDUCT REDUCTION IN THE PRESENCE OF CHLOROPHYLLIN (CHL) IS INDEPENDENT OF BP-METABOLIZING CYTOCHROME P450 INDUCTION IN HUMAN MCL-5 CELLS. Divi, RL¹, Orozco, CC¹, Weston, A², Poirier, MC¹. ¹National Cancer Institute, NIH, Bethesda, MD, United States, ²National Institute for Occupational Safety and Health, CDC, Morgantown, WV, United States.

#277 DNA LESION-SPECIFIC INTERACTION AND CO-LOCALIZATION OF REPLICATION PROTEIN A (RPA) AND THE MRE11/RAD50/NBS1 (MRN) COMPLEX AT STALLED REPLICATION FORKS AND IN REPAIR FOCI. Robison, JG¹, Bissler, JJ², Dixon, K³. ¹University of Cincinnati, Cincinnati, OH, United States, ²Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, United States, ³University of Arizona, Tucson, AZ, United States.

#278 RODENT BONE-MARROW MICRONUCLEUS TESTS WITH CENTROMERE LABELLING FOR THE DETECTION OF ANUEGENS. Doherty, AT, Hayes, J, Evans, S, O Donovan, M. AstraZeneca, Macclesfield, Cheshire, United Kingdom.

#279 THE RAD6 GROUP GENES MEDIATE ACCURATE REPAIR OF DOUBLE-STRANDED GAPS IN PLASMID DNA IN YEAST. Eckardt-Schupp, F, Steininger, S, Ahne, F, Moertl, S. GSF-National Research Center, Neuherberg, Germany.

#280 CELL CYCLE REGULATION AND LOCALIZATION OF MISMATCH REPAIR PROTEINS. Edelbrock, MA, Schroering, AS, Richards, TJ, Williams, KJ. Medical Universtiy of Ohio, Toledo, OH, United States.

#281 INTERLABORATORY VALIDATION OF A STUDY PROTOCOL FOR THE CONDUCT OF THE SYRIAN HAMSTER EMBRYO (SHE) CELL MICROWELL SCREENING MICRONUCLEUS ASSAY IN VITRO. Hu, T², Erexson, GL¹, Aardema, MJ², Farabaugh, CS¹, Yung, KM¹, Hsu, B¹, Stojhovic, GP¹. ¹Covance Laboratories, Inc., Vienna, VA, United States, ²Procter & Gamble Co., Cincinnati, OH, United States.

#282 THE ROLE OF ANC1 IN RECOVERY FROM EXPOSURE TO DNA DAMAGING AGENTS. Erlich, RL², Samson, LD³. ¹MIT Department of Biology, Cambridge, MA, United States, ²MIT Center for Environmental Health Sciences, Cambridge, MA, United States, ³MIT Department of Biological Engineering, Cambridge, MA, United States.

#283 SPECIFIC CHROMOSOMAL FRAGMENTS IN HUMAN LYMPHOCYTE MICRONUCLEI: MITOMYCIN C PREFERENTIALLY BREAKS CHROMOSOME 9 NEXT TO THE CLASSICAL SATELLITE REGION. Falck, GC-M, Jarventaus, H, Norppa, H. Finnish Institute of Occupational Health, Helsinki, Finland.

#284 THE EFFICIENT GLOBAL REPAIR OF OXIDATIVE DNA BASE MODIFICATIONS REQUIRES BOTH POLY(ADP-RIBOSYL)ATION AND PROTEINS INVOLVED IN TRANSCRIPTION COUPLED REPAIR. Flohr-Beckhaus, C¹, Schulz, I¹, Radicella, JP², Epe, B¹. ¹Institute of Pharmacy, University of Mainz, Mainz, Germany, ²Département de Radiobiologie et Radiopathologie, CEA, Fontenay aux Roses, France.

#285 THE IMPORTANCE OF RAD51 FOR THE RECOMBINATION PROCESS IN Trypanosoma cruzi. Freitas, JM, Silva, CGR, Silva, DGP, Silva, CFT, Macedo, AM, Pena, SDJ, Teixiera, SMR, Machado, CR. UFMG, Belo Horizonte, Brazil.

#286 ACCUMULATION AND PERSISTENCE OF SPONTANEOUSLY ARISING MUTATIONS IN MOUSE SOMATIC STEM CELLS. Fujikawa, K, Kagawa, N. Kinki University, Higashiosaka, Japan.

#287 OsEXO-1, A PLANT HOMOLOGUE OF CLASS III RAD2 NUCLEASE FAMILY MEMBER, IS CORRELATED WITH CELL PROLIFERATION. Furukawa, T, Shimada, H. Tokyo University of Science, Noda, Japan.

#288 INVESTIGATING THE MOLECULAR BASIS OF COCKAYNE SYNDROME: INTERACTION OF THE AMINO TERMINAL DOMAIN OF CSB WITH XPG. Fuss, J¹, Budworth, H¹, Ng, C¹, Lim, SY¹, McClean, CM¹, Tainer, JA², Cooper, PK¹. ¹Lawrence Berkeley National Laboratory, Berkeley, CA, United States, ²The Scripps Research Institute, La Jolla, CA, United States.

#289 CHARACTERIZATION OF THE SOS DEPENDENT MUTAGENESIS IN Caulobacter crescentus. Galhardo, RS, Rocha, RP, Marques, MV, Menck, CFM. Department of Microbiology, University of Sao Paulo, Sao Paulo, SP, Brazil.

#290 PROTECTIVE EFFECT OF CURCUMIN AND CHLOROPHYLLIN AGAINST DNA MUTATION INDUCED BY CYCLOPHOSPHAMIDE OR BENZO[a]PYRENE. Ghoneim, MA¹, Ibrahim, MA², Amer, HA², Elbehairy, AM². ¹Biotechnology Center, Fac. Vet. Med., Cairo University, Giza, Egypt, ²Dept. of Biochem.,Fac. Vet. Med., Cairo University, Giza, Egypt.

#291 MODEL STRUCTURE OF MITOCHONDRIAL DNA POLYMERASE GAMMA WITH 7,8-DIHYDRO-8-OXO-2’-DEOXYGUANOSINE—A COMMON MUTAGENIC DNA LESION. Graziewicz, MA¹, Bienstock, RJ², Copeland, WC¹. ¹Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States, ²Scientific Computing Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States.

#292 VIABILITY FROM GERM CELLS TO ADULTS DEPENDS MORE ON ERROR FREE DNA REPAIR THAN DOES VIABILITY FROM LARVAE TO ADULTS. Corredor, LE, Herrera, OL, Grigoriu de Buendia, P. Universidad Antonio Narinio, Bogota, Colombia.

#293 GENERATION OF BREAKAGE-PRONE REARRANGEMENT JUNCTIONS LEADS TO CHROMOSOMAL INSTABILITY. Allen, RN, Ritter, LE, Grosovsky, AJ. University of California at Riverside, Riverside, CA, United States.

#294 INHIBITION OF SPONTANEOUS MUTAGENESIS IN lacZ MICE. Khmelnitsky, M, Kosinska, W, Guttenplan, J. New York University, New York, NY, United States.

#295 INCORPORATION AND REPAIR OF [¹⁴C]8-Oxo-dG IN THE DNA OF MCF-7 BREAST CANCER CELLS USING ACCELERATOR MASS SPECTROMETRY. Hah, SS, Sumbad, RA, Henderson, PT. Lawrence Livermore National Laboratory, Livermore, CA, United States.

#296 HIGH-SPEED CONVERSION OF CYTOSINE TO URACIL IN BISULFITE GENOMIC SEQUENCING ANALYSIS OF DNA METHYLATION. Hayatsu, HH. Shujitsu University, Okayama, Japan.

#297 GENETIC POLYMORPHISMS IN DNA REPAIR AND FOLATE METABOLISM AND THEIR ASSOCIATION WITH CHROMOSOMAL ABERRATIONS. Heilimo, I¹, Siivola, P¹, Tuimala, J², Maunu, H¹, Jarventaus, H¹, Hirvonen, A¹, Metsola, K¹, Norppa, H¹. ¹Laboratory of Molecular and Cellular Toxicology, Department of Industrial Hygiene and Toxicology, Finnish Institute of Occupational Health, Helsinki, Finland, ²CSC, the Finnish IT Center for Science, Espoo, Finland.

#298 ANTIGENOTOXIC EFFECT OF NARINGIN ON MOUSE HEPATIC AND CARDIAC CELLS TREATED WITH DAUNORUBICIN. Hernández-Ceruelos, A¹, Madrigal-Bujaidar, E², Cariño-Cortés, R¹, Alvarez-González, I², Martino-Roaro, L². ¹Area Académica de Medicina, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, Mexico, ²Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Mexico City, D.F., Mexico.

#299 EFFECT OF POLYMORPHISMS IN THE Mgmt GENE ON PROTEIN ACTIVITY AS MEASURED BY A NOVEL FLUORESCENCE-BASED ASSAY. Hill, CE, Wickliffe, JK, Kinslow, CJ, Wolfe, KJ, Abdel-Rahman, SZ. University of Texas Medical Branch at Galveston, Galveston, TX, United States.

#300 CATALYTIC IMPAIRMENT AND DIMERIZATION OF POLYMORPHIC S326C OGG1 DNA REPAIR ENZYME. Hill, JW, Evans, MK. National Institute on Aging, Baltimore, MD, United States.

#301 MODULATION OF THE GENETIC ACTIVITY OF BLEOMYCIN BY AMINES IN AN ASSAY FOR MITOTIC GENE CONVERSION IN YEAST. Hoffmann, GR, Hughes, JF, Ronan, MV, Soron, GJ, Willett, CJ. Department of Biology, College of the Holy Cross, Worcester, MA, United States.

#302 THE DNA POLYMERASE LAMBDA IS REQUIRED FOR THE REPAIR OF NON-COMPATIBLE DNA DOUBLE STRAND BREAKS BY NHEJ IN MAMMALIAN CELLS. Capp, JP¹, Boudsocq, F¹, Bertrand, P², Laroche-Clary, A³, Pourquier, P³, Lopez, B², Cazaux, C¹, Canitrot, Y¹, Hoffmann, JS¹. ¹Equipe Instabilité Génétique et Cancer, IPBS-CNRS, Toulouse, France, ²UMR 217 CNRS-CEA, Fontenay aux Roses, France, ³INSERM E437, Bordeaux, France.

#303 INTERALLELIC HOMOLOGOUS RECOMBINATION AND TARGET INTEGRATION INDUCED BY DNA DOUBLE STRAND BREAKS. Honma, M, Takashima, Y, Sakuraba, T, Koizumi, T, Sakamoto, H, Hayashi, M. National Institute of Health Sciences, Tokyo, Japan.

#304 ANALYSIS OF ORF17, A MutT-TYPE ENZYME, IN VITRO AND IN VIVO. Hori, M¹, Fujikawa, K², Kasai, H², Harashima, H¹, Kamiya, H¹. ¹Grad. Sch. Pharm. Sci. Hokkaido Univ., Sapporo, Japan, ²Inst. Ind. Ecol. Sci. Univ. Occup. Env. Hlth., Kitakyuusyuu, Japan.

#305 CHARACTERIZATION OF GENETICALLY MODIFIED CHO UV5 CELL LINES FOR ASSESSING THE EFFECTS OF N-ACETYLTRANSFERASE 2 (NAT2) AND CYP1A2 ON AROMATIC AMINE GENOTOXICITY USING CELLOMICS AUTOMATED MICRONUCLEUS (MN) SCORING SYSTEM. Hu, T¹, Zhao, S², Gildea, LA¹, Tansky, CS¹, Neale, JR², Doll, MA², Hein, DW², Skare, JA¹, Aardema, MJ¹. ¹Procter & Gamble, Cincinnati, OH, United States, ²University of Louisville, Louisville, KY, United States.

#306 NONEQUIVALENT BIOLOGICAL CONSEQUENCES OF ENDOGENOUS AND RADIATION-INDUCED OXIDATIVE DNA LESIONS. Ide, H¹, Terato, H¹, Furusawa, Y². ¹Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan, ²National Institute of Radiological Sciences, Chiba, Chiba, Japan.

#307 FORMATION OF DNA ADDUCTS DERIVED FROM BIFUNCTIONAL NITROSAMINES. Ishikawa, S, Hatanaka, M, Mochizuki, M. Kyoritsu University of Pharmacy, Tokyo, Japan.

#308 MUTAGENIC ACTIVATION OF ETHYLENE DIBROMIDE BY CLONES FROM A SHUFFLED LIBRARY OF HUMAN AND RAT THETA CLASS GLUTATHIONE TRANSFERASES. Josephy, PD¹, Taylor, PL¹, Campbell, EFW¹, Mannervik, B². ¹University of Guelph, Guelph, Ontario, Canada, ²Uppsala University, Uppsala, Sweden.

#309 ROLE OF Atm GENE AS A DEFENCE FROM CHROMOSOME DAMAGE ARISING SPONTANEOUSLY AND AFTER RADIATION IN MICE. Kagawa, N¹, Shimura, M¹, Noda, Y², Tatsumi, K², Norimura, T³, Fujikawa, K¹. ¹Kinki University, Osaka, Japan, ²National Institute for Radiological Sciences, Chiba, Japan, ³University of Occupational & Environmental Health, Kitakyushu, Japan.

#310 CHECKPOINT RESPONSE TO REPLICATION STRESS: INDUCING TOLERANCE AND PREVENTING GENOMIC SEQUENCE DELETION. Kai, MK, Wang, TSFW. Stanford University, Stanford CA, United States.

#311 EFFECTS OF XPG MUTATIONS ON ENDONUCLEASE FUNCTION DO NOT CORRELATE WITH EFFECTS ON REPORTER GENE EXPRESSION FROM A UV-DAMAGED PLASMID. Kamiuchi, S, Sarker, A, Tsutakaw, S, Ison, M, Kwoh, E, Ng, C, Cooper, B, Campeau, E, Cooper, P. Lawrence Berkeley National Lab, Berkeley, CA, United States.

#312 IMPORTANT AMINO ACID RESIDUES INVOLVED IN SUBSTRATE RECOGNITION OF THE Escherichia coli 2-HYDROXY-DATPASE (ORF135). Kamiya, H¹, Iida, E¹, Satou, K¹, Mishima, M², Kojima, C², Harashima, H¹. ¹Grad. Sch. Pharm. Sci., Hokkaido Univ., Sapporo, Japan, ²Grad. Sch. Biol. Sci., Nara Inst. Sci. Tech., Ikoma, Nara, Japan.

#313 ANALYSIS OF TRANSLESION SYNTHESIS OF DNA ADDUCTS DERIVED FROM AIR POLLUTANT 3-NITROBENZANTHRONE AND ITS MUTAGENESIS. Kanno, T¹, Kawanishi, M², Takamura (Enya), T³, Fuchs, R⁴, Yagi, T². ¹Graduate School of Frontier Biosciences, Osaka Univ., Osaka, Japan, ²RIAST., Osaka Pref. Univ., Osaka, Japan, ³Div. Cancer Prevetntion, Natl. Cancer Inst., Tokyo, Japan, ⁴UPR-9003 du CNRS, Strasbourg, France.

#314 THE DDB1-CUL4A^DDB2 UBIQUITIN LIGASE, DEFICIENT IN XP-E PATIENTS, TARGETS HISTONE H2A FOR MONOUBIQUITINATION AT UV-DAMAGED DNA SITES. Kapetanaki, M, Guerrero-Santoro, J, Bisi, D, Levine, AS, Rapic Otrin, V. University of Pittsburgh School of Medicine, Molecular Genetics and Biochemistry, and Upci, Pittsburgh, United States.

#315 DISTINCT DAMAGE SPECIFICITIES OF HUMAN DNA GLYCOSYLASES NTH1, NEIL1, AND NEIL2. Katafuchi, A¹, Terato, H¹, Iwai, S², Hanaoka, F², Ide, H¹. ¹Hiroshima University, Hiroshima, Japan, ²Osaka University, Osaka, Japan.

#316 NUCLEOTIDE EXCISION REPAIR (NER) SYSTEM IS INVOLVED IN MONO ADP-RIBOSYLATED DNA ADDUCTS PRODUCED BY PIERISIN-1, A CYTOTOXIC PROTEIN FROM CABBAGE BUTTERFLY. Kawanishi, M¹, Matsukawa, K¹, Kuraoka, I³, Takamura, T², Totsuka, Y², Watanabe, M², Sugimura, T², Wakabayashi, K², Zou, Y⁴, Tanaka, K³, Yagi, T¹. ¹Osaka Prefecture University, Sakai, Osaka, Japan, ²National Cancer Center Research Institute, Tokyo, Japan, ³Osaka University, Suita, Osaka, Japan, ⁴East Tennessee State University, Johnson City, TN, United States.

#317 15-DEOXY-??????1^2,14-PROSTAGLANDIN J2 REGULATES P53 STABILITY BY ALTERING THE CONFORMATION OF P53 IN HUMAN BREAST CANCER (MCF-7) CELLS. Kim, D-H, Surh, Y-J. College of Pharmacy, Seoul National University, Seoul, South Korea.

#318 PREVENTION OF GENOMIC INSTABILITY IN BREAST CANCER CELLS BY THE DIETARY ANTIMUTAGENS GENISTEIN AND LYCOPENE. King, AA, Klein, CB. New York University School of Medicine, Tuxedo, NY, United States.

#319 CARCINOGENIC CR(VI) AND THE NUTRITIONAL SUPPLEMENT CR(III) INDUCE DNA DELETIONS IN YEAST AND MICE. Kirpnick, Z, Reliene, R, Schiestl, RH. UCLA, Los Angeles, CA, United States.

#320 INFLUENCE OF DNA REPAIR GENE POLYMORPHISMS ON THE YIELD OF CHROMOSOMAL ABERRATIONS. Kiuru, A¹, Lindholm, C¹, Heilimo, I², Ceppi, M³, Koivistoinen, A¹, Ilus, T¹, Hirvonen, A², Norppa, H², Salomaa, S¹. ¹STUK-Radiation and Nuclear Safety Authority, Helsinki, Finland, ²Finnish Institute of Occupational Health, Helsinki, Finland, ³National Cancer Research Institute, Genoa, Italy.

#321 DNA ALKYLATION REPAIR BY DNA DIOXYGENASES. Koivisto, PT¹, Robins, P², Lindahl, T², Sedgwick, B². ¹Finnish Institute of Occupational Health, Helsinki, Finland, ²Cancer Research UK, Hertfordshire, United Kingdom.

#322 ROLES OF REPLICATIVE AND SPECIALIZED DNA POLYMERASES IN FRAMESHIFT MUTAGENESIS: MUTABILITY OF Salmonella typhimurium STRAINS LACKING ONE OR ALL OF SOS-INDUCIBLE DNA POLYMERASES TO 26 CHEMICALS. Kokubo, K¹, Yamada, M¹, Kanke, Y², Nohmi, T¹. ¹National Institute of Health Sciences, Tokyo, Japan, ²Otsuma Women’s University, Tokyo, Japan.

#323 CHRONIC LOW DOSE RADIATION EXPOSURE LEADS TO DAMAGE ACCUMULATION AND PROFOUNDLY ALTERS DNA AND HISTONE METHYLATION IN MURINE THYMUS. Koturbash, I¹, Pogribny, I², Stevenson, S¹, Kovalchuk, O¹. ¹Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada, ²Division of Biochemical Toxicology, National Center for Toxicological Research, Jefferson, AR, United States.

#324 LIPID PEROXIDATION PRODUCT, TRANS-4-HYDROXY-2-NONENAL, FORMS SEQUENCE-SPECIFIC ADDUCTS TO P53 GENE, DNA-DNA AND DNA-PROTEIN CROSS-LINKS. Kowalczyk, P, Ciesla, JM, Tudek, B. Inst. Biochemistry&Biophysics PAS, Warszawa, Poland.

#325 EFFECTS OF DNA LESIONS ON TRANSCRIPTION ELONGATION BY RNA POLYMERASE II. Kuraoka, I, Suzuki, K, Tanaka, K. Osaka University, Osaka, Japan.

#326 ROLES OF WERNER SYNDROME PROTEIN IN NONHOMOLOGOUS END JOINING REPAIR. Kusumoto, R, Lee, JW, Bohr, VA. NIH, National Institute on Aging, Baltimore, MD, United States.

#327 THE ACTIVITY OF NUCLEOTIDE EXCISION REPAIR TOWARDS ADDUCTS FROM DIOL EPOXIDES OF DIFFERENT PAHS AND THE EFFECT OF CHLOROPHYLLIN AND BENZYLISOTHIOCYANATE ON THEIR CYTOTOXICITY. Lagerqvist, A, Erixon, K, Jenssen, D. Stockholm University, Stockholm, Sweden.

#328 WITHDRAWN

#329 P53 SUPPRESSION OVERWHELMS DNA POLYMERASE ??DEFICIENCY IN DETERMINING THE CELLULAR UV RESPONSE. Laposa, RR, Feeney, L, Crowley, E, Cleaver, JE. University of California, San Francisco, San Francisco, CA, United States.

#330 LATE H2AX HYPERPHOSPHORYLATION ASSOCIATED TO DECREASED DNA-PKCS EXPRESSION AND SIPS IN IRRADIATED SKIN FIBROBLASTS AND ENDOTHELIAL CELLS AND MODULATION BY PENTOXIFYLLINE AND TROLOX. Laurent, C¹, Delbos, M¹, Voisin, PA¹, Voisin, PH¹, Pouget, J-P². ¹IRSN, Fontenay-aux-Roses, France, ²INSERM EMI0227, Montpellier, France.

#331 EXPLORING THE HUMAN alkB HOMOLOGS AS POTENTIAL CANDIDATES FOR BONE MARROW GENE THERAPY. Lee, CY, Samson, LD. Massachusetts Institute of Technology, Cambridge, MA, United States.

#332 FUNCTIONAL ANALYSIS OF BRCA1 SNP HAPLOTYPES. Lee, J¹, Cho, M¹, Park, JY¹, Hasty, P¹, Vijg, J², Suh, Y¹. ¹Department of Molecular Medicine, Institute of Biotechnology, University of Texas Health Science Center, , San Antonio, TX, United States, ²Department of Physiology, University of Texas Health Science Center, San Antonio, TX, United States.

#333 MODULATION OF DNA END JOINING BY NUCLEAR PROTEINS. Liang, L¹, Deng, L¹, Chen, Y¹, Li, GC², Shao, C¹, Tischfield, JA¹. ¹Rutgers University, Piscataway, NJ, United States, ²Memorial Sloan-Kettering Cancer Center, New York, NY, United States.

#334 EVIDENCE THAT THE HUMAN HOMOLOG OF THE S. cerevisiae REV7 PROTEIN PLAYS AN ESSENTIAL ROLE IN MUTAGENIC TRANSLESION SYNTHESIS PAST FORK-BLOCKING LESIONS IN HUMAN FIBROBLAST CELLS. McNally, K, McCormick, JJ, Maher, VM. Michigan State University, East Lansing, MI, United States.

#335 NUCLEOTIDE EXCISION REPAIR MUTANTS SHOW IMPAIRED H2AX PHOSPHORYLATION AFTER EXPOSURE TO IONIZING RADIATION. Marti, TMM, Hefner, EH, Feeney, LF, Cleaver, JEC. University, San Francisco, CA, United States.

#336 INDUCED CHROMOSOME BREAKPOINTS DISTRIBUTION DEPENDS ON REPLICATION TIMING OF EU/HETEROCHTROMATIC REGIONS IN CHO9 CELLS. Di Tomaso, MVDT, Martínez-López, WM-L. ¹Instituto Clemente Estable, Montevideo, Uruguay, ²Faculty of Sciences, Montevideo, Uruguay.

#337 NON-HOMOLOGOUS END-JOINING IN X-RAY-IRRADIATED SCID/gpt DELTA TRANSGENIC MOUSE. Masumura, K¹, Hoshino, M¹, Yatagai, F², Ochiai, M³, Nakagama, H³, Nohmi, T¹. ¹National Institute of Health Sciences, Tokyo, Japan, ²Institute of Physical and Chemical Research (RIKEN), Saitama, Japan, ³National Cancer Center Research Institute, Tokyo, Japan.

#338 CATALYTIC AND DAMAGE RECOGNITION MECHANISMS OF HUMAN SMUG1. Matsubara, M, Tanaka, T, Terato, H, Ide, H. Hirhosima University, Higashi-Hiroshima, Hiroshima, Japan.

#339 DEVELOPMENT OF A NOVEL SITE-SPECIFIC MUTAGENESIS ASSAY USING MALDI-TOF MS. McLuckie, KIE, Jones, DJL, Lamb, JH, Sandhu, JK, Brown, K, Farmer, PB. Cancer Biomarkers and Prevention Group, The Biocentre, University of Leicester, University Road, Leicester, United Kingdom.

#340 LOCALIZED INDUCTION OF UV PHOTOPRODUCTS IN CELL NUCLEAR DNA BY THREE-PHOTON NEAR INFRA-RED RADIATION. Meldrum, RA¹, Topley, S², Botchway, SW², Hirst, GJ². ¹Univeristy Of Birmingham, Birmingham, United Kingdom, ²CCLRC Rutherford Appleton Laboratories, Oxford, United Kingdom.

#341 CELLULAR RESPONSES TO DNA DAMAGE INDUCED BY CISPLATIN IN FIBROBLAST CELL LINES, NORMAL AND DEFICIENT FOR ATM GENE. Mello, SS¹, Fachin, AL¹, Junta, CM¹, Sandrin-Garcia, P¹, Passos, GAS², Donadi, EA³, Sakamoto-Hojo, ET⁴. ¹Departamento de Genetica – Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil, ²Faculdade de Odontologia de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil, ³Clinica Medica, Faculdade de Medicina de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil, ⁴Departamento de Biologia – Faculdade de Filosofia, Ciencias e Letras de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, SP, Brazil.

#342 ANALYSIS OF LOH IN Tk^+/- MICE TREATED WITH ANTIRETROVIRAL NUCLEOSIDE ANALOGUE DRUGS. Mittelstaedt, RA, Shaddock, JG, Dobrovolsky, VN, Von Tungeln, LS, Beland, FA, Heflich, RH. USFDA/National Center for Toxicological Research, Jefferson, AR, United States.

#343 PLASTID DNA POLYMERASES FROM HIGHER PLANTS, Arabidopsis thaliana. Mori, Y¹, Kimura, S², Saotome, A¹, Kasai, N¹, Sakaguchi, N¹, Uchiyama, Y¹, Ishibashi, T⁴, Yamamoto, T³, Sakaguchi, K¹. ¹Tokyo University of Science, Chiba, Japan, ²University of California, Davis, San Francisco, CA, United States, ³Children’s Hospital Oakland Research Institute, San Francisco, CA, United States, ⁴University of Victoria, Victoria, Chile.

#344 MUTATION IN THE PHOSPHATIDYL INOSITOL GLYCAN - COMPLEMENTATION GROUP A (PIG-A) GENE. METHODS DEVELOPMENT FOR DETERMINING PIG-A MUTANT FREQUENCY IN HUMAN LYMPHOBLASTOID CELLS BY FLOW CYTOMETRY. Morris, SM¹, McGarrity, LJ¹, Domon, OE¹, Mittelstaedt, RA¹, Heflich, RH¹, Albertini, RJ². ¹National Center for Toxicological Research, Jefferson, AR, United States, ²University of Vermont, Burlington, VT, United States.

#345 THE FUNCTIONAL CONSEQUENCES OF ACETYLATION ON WRN PROTEIN. Muftuoglu, M, Kusumoto, R, von Kobbe, C, Bohr, VA. National Intitutes of Health, National Institute on Aging, Laboratory of Molecular Gerontology, Baltimore, MD, United States.

#346 DIETARY ANTIMUTAGENS REDUCE SPECIFIC TYPES OF SPONTANEOUS DNA DAMAGE AND ALTER GENE EXPRESSION IN MISMATCH REPAIR DEFICIENT HUMAN CELLS. Mure, K¹, Shaughnessy, DT², King, A³, Takeshita, T¹, Ducharme, DM⁴, Rossman, TG³, Klein, CB³. ¹Wakayama Medical University School of Medicine, Department of Public Health, Wakayama City, Wakayama, Japan, ²Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States, ³New York University School of Medicine, Nelson Institute of Environmental Medicine, Tuxedo, NY, United States, ⁴Microarray Group, National Institute of Environmental Health Sciences, Research Triangle Park, NC, United States.

#347 MAINTENANCE OF GENOMIC STABILITY AT G/C-RICH REPETITIVE DNA SEQUENCES. Nakagama, H, Higuchi, H, Tanaka, E, Nagao, M, Fukuda, H. National Cancer Center Research Institute, Tokyo, Japan.

#348 GENOTOXIC POTENTIAL AND REPAIR MECHANISMS OF OXANINE AND OXANINE CROSS-LINK LESIONS INDUCED BY NITROSATIVE STRESS. Nakano, T¹, Terato, H¹, Houten, B², Suzuki, T³, Ide, H¹. ¹Hiroshima University, Higashi-Hiroshima, Hiroshima, Japan, ²NIH/NIEHS, Research Triangle Park, NC, United States, ³Shujitu University, Nishigawara, Okayama, Japan.

#349 HIGH FREQUENCIES OF CROSSING-OVER ASSOCIATED WITH LONG TRACT GENE CONVERSION IN HUMAN CELLS. Neuwirth, EAH¹, Honma, M², Grosovsky, AJ¹. ¹University of California, Riverside, Riverside, CA, United States, ²Japanese National Institute of Health Sciences, Setagya, Tokyo, Japan.

#350 ALKB PROTECTION AGAINST MMS-INDUCED MUTAGENESIS IN E. coli. EFFECT OF UmuD(D’)C AND AlkA PROTEINS. Nieminuszczy, J, Sikora, A, Maciejewska, A, Wrzesinski, M, Janion, C, Grzesiuk, E. Inst. Biochemistry & Biophysics PAS, Warszawa, Poland.

#351 MUTAGENIC SPECIFICITY OF N-ACETOXY-3-AMINOBENZANTHORONE, A DERIVATIVE OF 3-NITROBENZANTHORONE. Nishida, H¹, Kawanishi, M¹, Takamura, T², Wakabayashi, K², Yagi, T¹. ¹Osaka Pref. Univ., Osaka, Japan, ²Cancer Center Res. Inst., Tokyo, Japan.

#352 DRUG-MODIFIED HOMOLOGOUS DNA EQUALS HOMEOLOGOUS DNA. Nowosielska, A, Calmann, MA, Marinus, MG. University of Massachusetts Medical School, Worcester, MA, United States.

#353 HCC1937 BREAST CANCER CELLS ARE DEFICIENT IN THE REPAIR OF 8-HYDROXYGUANINE. Nyaga, SG¹, Lohani, A¹, Trzeciak, A¹, Barnes, J¹, Jaruga, P², Dizdaroglu, M³. ¹NIA/NIH, Baltimore, MD, United States, ²Univ. Of Maryland, Baltimore, MD, United States, ³NIST, Gaithersburg, MD, United States.

#354 MUTAGENESIS IN DNA REPAIR-DEFICIENT MUTANTS OF THE EXTREMELY THERMOPHILIC EUBACTERIUM Thermus thermophilus HB27. Ohta, T. Tokyo Univ. Pharmacy and Life Science, Tokyo, Japan.

#355 THE MUTAGENICITY OF 7,8-DIHYDRO-8-OXOGUANINE IN YEAST AND INHIBITORY ACTION OF DNA POLYMERASE ETA AGAINST IT ARE BOTH SEQUENCE DEPENDENT. Okugawa, Y¹, Otsuka, C¹, Loakes, D², Negishi, K¹. ¹Advanced Science Research Center, Okayama University, Okayama, Japan, ²Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.

#356 RECQ HELICASES AND DNA REPAIR PATHWAYS AT TELOMERIC DNA. Opresko, PL², Muftuoglu, M¹, Mason, P¹, Wilson, DM¹, Bohr, VA¹. ¹Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD, United States, ²Department of Environmental and Occupational Health, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA, United States.

#357 ROLES OF THE POLYMERASE AND BRCT DOMAINS OF REV1 PROTEIN IN TRANSLESION DNA SYNTHESIS IN YEAST IN VIVO. Otsuka, C¹, Kunitomi, N¹, Iwai, S², Loakes, D³, Negishi, K¹. ¹Department of Genomics and Proteomics, Okayama University Advanced Science Research Center, Okayama, Japan, ²Division of Chemistry, Graduate School of Engineering Science, Osaka University, Osaka, Japan, ³Medical Research Council, Laboratory of Molecular Biology, Cambridge, United Kingdom.

#358 RADIATION-INDUCED CYTOGENETIC EFFECTS IN ONE CELL EMBRYOS PRODUCED BY Parp-1 KNOCKOUT OR WILD-TYPE FEMALE MICE. Pacchierotti, F, Ranaldi, R. ENEA, Roma, Italy.

#359 DNA DAMAGE AND REPAIR TESTS IN PREDICTION OF CANCER THERAPY. Palyvoda O¹, Auner GW¹, Rzeszowska-Wolny J². ¹Smart Sensors and Integrated Microsystems, College of Engineering, Wayne State University, Detroit, MI, United States, Department of Experimental and Clinical Radiobiology, ²Center of Oncology, Maria Sklodowska-Curie Memorial Institute, Gliwice, Poland

#360 AMNIOTIC FLUID OF RATS DOSED ORALLY WITH CD AND PB CAUSED GENOTOXICITY ON HUMAN LYMPHOCYTES. Park, EJ¹, Jeon, KI¹, Byun, BH², Lee, KH³, Choi, JH⁴. ¹Dept. of Food and Nutrition, Kyungnam University, Masan, South Korea, ²Dept. of Oriental Medicine, Daegu Haany University, Daegu, South Korea, ³Dept. of Food and Nutrition, Changwon National University, Changwon, South Korea, ⁴Div. of Food Science, Jinju International University, Jinju, South Korea.

#361 HEMATOPOIETIC STEM CELL DEFECT CAUSED BY LOSS OF FUNCTIONAL DNA-PKCS. Park Y¹, Lin Y¹, Gerson SL^1,2,3,4. ¹Case Western Reseve University, Cleveland, OH, United States, ²Case Comprehensive Cancer Center, Cleveland, OH, United States, ³Center of Stem Cell and Regenerative Medicine, Cleveland, OH, United States, ⁴University Hospitals of Cleveland, Cleveland, OH, United States.

#362 GENERATION OF 1100DELC CHK2 POLYMORPHISM IN ‘KNOCK-IN’ MICE. Penner, CG, Stambrook, PJ. University of Cincinnati, Cincinnati, OH, United States.

#363 COMPLEMENTATION ANALYSIS OF RAD51D ALTERNATIVE SPLICE VARIANTS. Gruver, AM, Pittman, DL. Medical University of Ohio, Toledo, Ohio, United States.

#364 GENETIC DAMAGE CAUSED BY A VARIETY OF CARCINOGENS AS MEASURED AT A TANDEMLY REPEATED DNA LOCUS. Polyzos, A¹, Parfett, C¹, Healy, C¹, Williams, A², Douglas, G¹, Yauk, C¹. ¹Environmental and Occupational Toxicology Division, HECSB, Ottawa, Ontario, Canada, ²Biostatistics and Epidemiology Division, HECSB, Ottawa, Ontario, Canada.

#365 MODIFICATION OF CONSTITUTIVE TP53 mRNA EXPRESSION AND RADIATION INDUCED DNA REPAIR BY THE ARG72PRO POLYMORPHISM OF TP53. Popanda, O, Marquardt, J, Woelfelschneider, A, Zelezny, O, Bartsch, H, Schmezer, P. German Cancer Research Center, DKFZ, Heidelberg, Germany.

#366 COMPARISON OF GENOTOXIC EFFECTS USING THE COMET ASSAY IN TISSUES OF FEMALE CYP2E1^-/- AND WILD-TYPE MICE TREATED WITH ACRYLAMIDE: EVIDENCE CONSISTENT WITH A GLYCIDAMIDE-MEDIATED EFFECT. Recio, L¹, Witt, KL², Kissling, GE³, Tice, R¹, Ghanayem, B⁴. ¹ILS, Inc, Research Triangle Park, NC, United States, ²Environmental Toxicology Program NIEHS, Research Triangle Park, NC, United States, ³Biostatistics Branch NIEHS, Research Triangle Park, NC, United States, ⁴Laboratory of Pharmacology and Chemistry NIEHS, Research Triangle Park, NC, United States.

#367 P53 PHOSPHORYLATED ON SERINE 15 DIFFERENTLY ASSOCIATED WITH KEY ENZYMES OF HOMOLOGOUS RECOMBINATION. Restle, A, Janz, C, Wiesmüller, L. Universitäts-Frauenklinik, Ulm, Germany.

#368 DNA DAMAGE AND ANTIOXIDANT STATUS IN PERIPHERAL BLOOD LYMPHOCYTES FROM BREAST CANCER PATIENTS. Agnoletto, M², Ghecheva, T², Oliveira, AF⁴, Franke, F⁴, Cassini, C³, Salvador, M³, Henriques, JAP², Saffi, J¹. ¹Lutheran University of Brazil - ULBRA, Canoas, RS, Brazil, ²Federal University of Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil, ³Biotechnology Center, Caxias do Sul, RS, Brazil, ⁴Center for Cancer Treatment, Ijuí, RS, Brazil.

#369 ALTERATIONS IN GENE EXPRESSION PROFILES, AS EVALUATED BY DNA MICROARRAYS, IN MAMMALIAN CELLS EXPOSED TO IONIZING RADIATION. Sakamoto-Hojo, ET², Fachin, AL¹, Merchi, IM¹, Cardoso, RS¹, Junta, CM¹, Sandrin-Garcia, P¹, Mello, SS¹, Donadi, EA⁴, Passos, GAS³. ¹Departamento de Genética, Faculdade de Medicina deRibeirão Preto, USP, Ribeirao Preto, SP, Brazil, ²Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, USP, Ribeirão Preto, SP, Brazil, ³Faculdade de Odontologia de Ribeirão Preto, USP, Ribeirao Preto, SP, Brazil, ⁴Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, USP, Ribeirao Preto, SP, Brazil.

#370 DNA-PKCS-DEPENDENT RECRUITEMENT OF ARTEMIS AT DNA DOUBLE-STRAND BREAKS. Salles, B¹, Drouet, J¹, Delteil, C¹, de Villartay, JP², Calsou, P¹. ¹IPBS CNRS/univ, Toulouse, France, ²U429 INSERM, Paris, France.

#371 LYCOPENE INHIBITS DIETHYLNITROSAMINE-INDUCED DNA DAMAGE BUT NOT GST-P POSITIVE FOCI IN RAT HEPATOCYTES. Scolastici, C, Alves de Lima, RO, Barbisan, LF, Ferreira, ALA, Salvadori, DMF. UNESP, Botucatu, SP, Brazil.

#372 MOLECULAR CHARACTERIZATION OF RecQ HOMOLOGUES FROM RICE (Oryza sativa L. cv. Nipponbare). Saotome, AS¹, Kimura, SK², Morohashi, KM¹, Mori, YM¹, Sakaguchi, KS¹. ¹Tokyo University of Science, Noda-shi, Chiba, Japan, ²Section of Plant Biology, University of California, Davis, CA, United States.

#373 FUNCTIONAL INTERACTION BETWEEN XPG AND CAF-I. Sarker, A, Tsutakawa, S, Chernikova, S, Chan, D, Qin, J, Matsumoto, Y, Kaufman, PD, Cooper, PK. Lawrence Berkeley National Lab, Berkeley,CA, United States.

#374 DNA DAMAGE SENSING ENZYME, POLY(ADP-RIBOSE) POLYMERASE-1, IS A NEGATIVE REGULATOR OF HIV-1 TRANSCRIPTION THROUGH COMPETITIVE BINDING TO TAR RNA WITH TAT-P-TEFB COMPLEX. Parent, M, Yung, TMC, Wada, T, Handa, H, Satoh, MS. CHUL Research Center and Laval University, Ste-Foy, Quebec, Canada.

#375 ROLES OF THE Escherichia coli DINB AND UMUDC PROTEINS IN MUTATIONS INDUCED BY OXIDIZED DNA PRECURSORS. Satou, K¹, Yamada, M², Nohmi, T², Harashima, H¹, Kamiya, H¹. ¹Grad. Sch. Pharm. Sci., Hokkaido Univ., Sapporo, Hokkaido, Japan, ²Natl. Inst. Hlth. Sci., Tokyo, Japan.

#376 SOMATIC MICROINDELS: AN ECLECTIC MIX OF MUTATIONAL MECHANISMS. Scaringe, W¹, Gonzalez, K¹, Hill, K², Li, X¹, Li, K¹, Wang, J¹, Sommer, S¹. ¹City of Hope/Beckman Research Institute, Duarte, CA, United States, ²University of Western Ontario, London, ON, Canada.

#377 A NOVEL MUTATOR PATHWAY IN Escherichia coli. Gawel, D, Hamilton, M, Schaaper, RM. National Institute of Environmental Health Sciences, Research Triangle Park, United States.

#378 THE EFFECT OF ANTIOXIDANTS ON GENETIC INSTABILITY AND CANCER IN ATAXIA TELANGIECTASIA. Reliene, R, Schiestl, RH. UCLA, Los Angeles, CA, United States.

#379 MISMATCH REPAIR AND HPMS2 POST-TRANSLATIONAL PHOSPHORYLATION. Schroering, AG, Williams, KJ. Medical University of Ohio, Toledo, OH, United States.

#380 STUDIES ON THE GENOTOXIC AND MUTAGENIC MECHANISM OF PATULIN IN CULTURED CELLS. Schumacher, DM, Metzler, M, Lehmann, L. University of of Karlsruhe, Institute of Applied Biosciences, Karlsruhe, Germany.

#381 DISCRIMINATORY EFFECT OF RAD51 RECOMBINATIONAL PROTEIN BETWEEN TRANSCRIPTIONALLY ACTIVE AND INACTIVE CHROMATIN. Serrano de la Peña, L, Cui, E, Tischfield, JA. Rutgers University, Piscataway, NJ, United States.

#382 METHYLEUGENOL IS A TRANSSPECIES IN VIVO MUTAGEN CAUSING TANDEM BASE SUBSTITUTIONS. Shane, BS², Tyrrell, SP¹, Cunningham, MC². ¹Louisiana State University, Baton Rouge, LA, United States, ²NIEHS, Research Triangle Park, NC, United States.

#383 FORMATION OF MICRONUCLEI FROM AGGREGATED DNA STRAND BREAKS DURING INTERPHASE. Shao, C, Chen, Y, Tischfield, JA. Rutgers University, Piscataway, NJ, United States.

#384 COORDINATE INTERACTIONS OF WRN AND PCNA WITH FEN-1 THAT MODULATE FEN-1 CATALYTIC ACTIVITIES. Sharma, S¹, Sommers, J¹, Gary, R², Hübscher, U³, Brosh, R¹. ¹Laboratory of Molecular Gerontology, National Institute on Aging, NIH, Baltimore, MD, United States, ²Department of Chemistry, University of Nevada Las Vegas, Las Vegas, NV, United States, ³Institute of Veterinary Biochemistry and Molecular Biology, University of Zurich, ZURICH, Switzerland.

#385 THE ROLE OF BRCA1 IN DNA REPAIR AND CHEMOSENSITIVITY. Sharma, VB, Rajapaksa, SM, Ford, JM. Stanford University, Stanford, CA, United States.

#386 INCREASED MUTATIONS IN Parp-1 KNOCKOUT MICE AFTER TREATMENT WITH AN ALKYLATING AGENT AND WITH AGING. Shibata, A¹, Nohmi, T⁴, Teraoka, H³, Nakagama, H², Sugimura, T², Suzuki, H⁵, Masutani, M¹. ¹National Cancer Center Research Institute, ADP-ribosylation in Oncology Project, Tokyo, Japan, ²National Cancer Center Research Institute, Biochemistry Devision, Tokyo, Japan, ³Tokyo Medical and Dental University, Pathologic Biochemistry, Tokyo, Japan, ⁴National Institute of Health Sciences, Division of Genetics and Mutagenesis, Tokyo, Japan, ⁵Obihiro University of Agriculture and Veterinary Medicine, National Research Center for Ptotozoan DIseases, Obihiro, Japan.

#387 DROSOPHILA DAMAGED DNA BINDING PROTEIN 1 CONTRIBUTES TO DNA REPAIR IN SOMATIC CELLS. Shimanouchi, K¹, Takata, K², Yamaguchi, M³, Murakami, S¹, Takeuchi, R¹, Kanai, Y¹, Ruike, T¹, Nakamura, R¹, Abe, Y¹, Sakaguchi, K¹. ¹Tokyo University of Tokyo, Noda-shi, Chiba, Japan, ²University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, PA, United States, ³Kyoto Institute of Technology, Kyoto-shi, Kyoto, Japan.

#388 THE HIDDEN MUTATOR PHENOTYPE: RNA SPLICING FIDELITY OF HUMAN BASE EXCISION REPAIR GENES. Skandalis, A, Disher, K. Brock University, St. Catharines, Ontario, Canada.

#389 Mlh1-DEPENDENT RESPONSES TO 2-AMINO-1-METHYL-6-PHENYLIMIDAZO [4,5-B] PYRIDINE (PhIP), A FOOD-BORNE CARCINOGEN. Smith-Roe, SL, Buermeyer, AB. Oregon State University, Corvallis, OR, United States.

#390 THE DIVERSE EFFECTS OF ARSENIC ON THE MULTI FUNCTIONAL PROTEIN REF-1/ AP ENDONUCLEASE. Sykora, P, Snow, ET. Deakin University, Burwood, Victoria, Australia.

#391 DNA INTERACTIONS IN A WELL-POSITIONED NUCLEOSOME CONTAINING SITE-SPECIFIC UV-LESIONS AND THEIR EFFECT ON DNA REPAIR RATES. Svedruzic, Z, Smerdon, MJ. Washington State University, Pullman WA, United States.

#392 MUTAGENICITY IN SALMONELLA OF SULFUR-CONTAINING POLYCYCLIC AROMATIC HETEROCYCLES AND THEIR DIHYDRODIOL DERIVATIVES. Swartz, CD¹, Nesnow, S², Sikka, HC³, Kumar, S³. ¹University of North Carolina, Chapel Hill, NC, United States, ²Environmental Carcinogenesis Division, USEPA, Research Triangle Park, NC, United States, ³Environmental Toxicology and Chemistry Laboratory, SUNY Buffalo, Buffalo, NY, United States.

#393 ACTIVITY, BUT NOT EXPRESSION OF OGG1 GLYCOSYLASE IS DECREASED IN TUMORS OF LUNG CANCER PATIENTS. Swoboda, M¹, Speina, E¹, Janik, J¹, Zaim, J¹, Tudek, B¹, Gackowski, D², Siomek, A², Kowalewski, J², Olinski, R¹. ¹Institute of Biochemistry and Biophysics PAS, Warsaw, Poland, ²Ludwig Rydgier Medical University, Bydgoszcz, Poland.

#394 SYNTHESIS OF SITE-SPECIFIC MODIFIED OLIGONUCLEOTIDES WITH PhIP. Takamura, T¹, Ishikawa, S², Mochizuki, M², Sugimura, T¹, Wakabayashi, K¹. ¹NCCRI, Tokyo, Japan, ²Kyoritsu Univ. Pharm., Tokyo, Japan.

#395 DNA DOUBLE STRAND BREAK REPAIR AND CELL CYCLE IN A HUMAN LYMPHOBLASTOID CELL LINE. Takashima, Y, Sakuraba, M, Koizumi, T, Sakamoto, H, Hayashi, M, Honma, M. National Institute of Health Sciences, Tokyo, Japan.

#396 DROSOPHILA DNA POLYMERASE ZETA INTERACTS WITH RECOMBINATION REPAIR PROTEIN 1, THE DROSOPHILA HOMOLOGUE OF HUMAN AP ENDONUCLEASE 1. Takeuchi, R, Ruike, T, Nakamura, R, Shimanouchi, K, Kanai, Y, Abe, Y, Ihara, A, Sakaguchi, K. Tokyo University of Science, Noda-shi, Chiba-ken, Japan.

#397 Msh2 DEFICIENCY REVERTS SENSITIVITY AND PROMOTES S-PHASE PROGRESSION OF UVC-DAMAGED XPA-DEFICIENT CELLS. Takeuchi, S², Ichikawa, M¹, Yoshino, M¹, Matsuda, T¹, Nakatsu, Y³, Riele, HT⁴, Tanaka, K¹. ¹Human Cell Biology Group, Laboratories for Organismal Biosystems, Graduate School of Frontier Biosciences, Osaka University, Osaka, Japan, ²Morphological and Physiological Sciences, University of Fukui Faculty of Medical Sciences, Fukui, Japan, ³Department of Medical Biophysics and Radiation Biology, Faculty of Medical Sciences, Graduate Schools, Kyushu University, Fukuoka, Japan, ⁴Division of Molecular Biology, The Netherlands Cancer Institute, Amsterdam, Netherlands.

#398 IMPLICATIONS OF NEIL1’S INTERACTION WITH PCNA AND RPA: POSSIBLE ROLE OF NEIL1 IN REPLICATION-ASSOCIATED REPAIR. Theriot C¹, Dou H¹, Kruciak T¹, Hazra T¹, Mitra S¹. ¹Department of Human Biological Chemistry and Genetics, University of Texas Medical Branch, Galveston, TX, United States.

#399 NEW INSIGHT INTO THE TOPO-SELECTIVE REPAIR OF O⁶-ALKYLGUANINES IN DNA: INVOLVEMENT OF DIFFERENT MECHANISMS AND PROTECTIVE EFFECTS IN CARCINOGENESIS. Engelbergs, J¹, Sugasawa, K², Boehm, B¹, Tanaka, K³, Kamino, K⁴, Hanaoka, F², Thomale, J¹. ¹University of Essen Medical School, Essen, Germany, ²RIKEN Institute, Saitama, Japan, ³Osaka University, Osaka, Japan, ⁴Medical University Hannover, Hannover, Germany.

#400 EMBRYONIC STEM CELLS MAY PRESERVE GENOMIC INTEGRITY BY USING HOMOLOGY-MEDIATED PATHWAYS FOR DNA REPAIR. Tichy, ED, Stambrook, PJ. University of Cincinnati, Cincinnati, OH, United States.

#401 G4 DNA STRUCTURES GENERATED IN THE NON-TRANSCRIBED STRAND DURING TRANSCRIPTION POSE BLOCKS TO T7 RNA POLYMERASE AND MAMMALIAN RNA POLYMERASE II. Park-Snyder, S¹, Tornaletti, S¹, Duquette, ML², Maizels, N², Hanawalt, PC¹. ¹Stanford University, Stanford, CA, United States, ²University of Washington, Seattle, WA, United States.

#402 INTERACTION MAPPING OF A MULTIFUNCTIONAL HUMAN DNA REPAIR PROTEIN XPG WITH DNA GLYCOSYLASES HNTH1 AND NEIL2 IN REPAIR OF OXIDATIVE DNA DAMAGE. Tsai, MS¹, Cho, MT¹, Win, KZ¹, Tsutakawa, SE¹, Sarker, AH¹, Mitra, S², Hazra, TK², Cooper, PK¹. ¹Lawrence Berkeley National Laboratory, Berkeley, CA, United States, ²Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, TX, United States.

#403 REPAIR PATHWAYS OF LIPID PEROXIDATION PRODUCT, TRANS-4-HYDROXY-2-NONENAL ADDUCTS TO DNA BASES IN BACTERIAL AND MAMMALIAN CELLS. Tudek, B¹, Rusin, B¹, Maddukuri, L¹, Komisarski, M¹, Kusmierek, J¹, Stevnsner, TV², Zdzienicka, M³. ¹Inst. Biochemistry and Biophysics PAS, Warszawa, Poland, ²Danish Center for Molecular Gerontology Department of Molecular Biology University of Arhus, Arhus, Denmark, ³Department of toxicogenetics University Medical Center Wassenarseweg, Leiden, Netherlands.

#404 MUTAGENIC RESPONSE OF ?IX174 TRANSGENIC MOUSE EMBRYONIC CELL LINE PX-2 TO UVB IRRADIATION. Valentine, CR, Hass, BS, Rainey, HF. National Center for Toxicological Research/FDA, Jefferson, AR, United States.

#405 THE FLEMISH HEALTH AND ENVIRONMENT BIOMONITORING PROGRAM: EXPOSURE OF NEONATES MEASURED IN CORD BLOOD. Koppen, G¹, Neelen, V², Bruckers, L³, Bilau, M⁴, Van De Mieroop, E², Brits, E¹, Schroijen, C⁵, Baeyens, W⁵, van Larebeke, N⁴, Schoeters, G¹. ¹Flemish Institute of Technological Research, Mol, Belgium, ²Provincial Institute for Hygiene, Antwerp, Belgium, ³Limburgs Universitair Centrum, Diepenbeek, Belgium, ⁴Ghent University, Ghent, Belgium, ⁵Vrije Universiteit Brussel, Brussel, Belgium, ⁶University of Antwerp, Antwerp, Belgium.

#406 UV-DDB MEDIATES EFFICIENT TARGETING OF THE NER COMPLEX TO UV-INDUCED LESIONS AND ENHANCED Ddb2 EXPRESSION PROTECTS MICE FROM CARCINOGENIC EFFECTS OF CHRONIC UV-B IRRADIATION. Alekseev, S, Rebel, H, Moser, J, Fousteri, M, de Gruijl, FR, Vrieling, H, van Zeeland, AA, Mullenders, LHF. Leiden University Medical Center, Leiden, Netherlands.

#407 EVIDENCE FOR DISTINCT MECHANISMS OF Z-DNA-STIMULATED GENETIC INSTABILITY IN MAMMALIAN CELLS. Wang, G, Christensen, L, Vasquez, K. U.T.M.D. Anderson Cancer Center, Smithville, TX, United States.

#408 SOMATIC MACROINDELS OCCUR FREQUENTLY IN YOUNG MICE. Wang, J¹, Gonzalez, K¹, Hill, K², Tsai, B¹, Scaringe, W¹, Sommer, S¹. ¹City of Hope/Beckman Research Institute, Duarte, CA, United States, ²University of Western Ontario, London, ON, Canada.

#409 DNA REPAIR PHENOTYPE USING THE COMET ASSAY AND CORRELATION WITH BASE EXCISION REPAIR GENE HAPLOTYPES. Watson, M, Coleman, J, Umbach, D, Xu, Z, Taylor, J. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, United States.

#410 Xpc-NULL MICE ARE SENSITIVE TO THE MUTAGENIC EFFECTS OF 3,4-EPOXY-1-BUTENE AND 1,3-BUTADIENE. Wickliffe, JK¹, Xie, J¹, Herring, SM¹, Ammenheuser, MM¹, Galbert, LA¹, Masters III, OE¹, Lloyd, RS², Friedberg, EC³, Ward Jr., JB¹. ¹University of Texas Medical Branch, Galveston, TX, United States, ²Oregon Health and Science University, Portland, OR, United States, ³University of Texas Southwestern Medical Center, Dallas, TX, United States.

#411 DEPLETION OF THE VERTEBRATE-SPECIFIC RAD51-INTERACTING PROTEIN RAD51AP1/PIR51 SENSITIZES HUMAN CELLS TO MMC AND TO X-RAYS. Wiese, C, Groesser, T, Collins, DW, Rydberg, B, Schild, D. Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, CA, United States.

#412 HOMOLOGY-DIRECTED DNA REPAIR: DIRECT REGULATORY ROLES OF THE TUMOR SUPPRESSOR P53. Wiesmüller, LW. Gynaecological University Hospital, Ulm, Germany.

#413 NON-HOMOLOGOUS END JOINING AND HOMOLOGOUS RECOMBINATION REPAIR PROTECT AGAINST PARTICULATE CHROMATE-INDUCED CHROMOSOME INSTABILITY. Wise, SS, Stackpole, MM, Camyre, E, Duzevik, EG, Wise Sr., JP. Wise Laboratory of Environmental and Genetic Toxicology, Maine Center for Toxicology and Environmental Health, Bioscience Research Institute, University of Southern Maine, Portland, ME, United States.

#414 FUNCTIONAL CHARACTERIZATION OF POLYMORPHISMS IN THE NUCLEOTIDE EXCISION REPAIR GENE XPD/ERCC2. Wolfe, KJ, Hill, CE, Wickliffe, JK, Abdel-Rahman, SZ. University of Texas Medical Branch, Galveston, TX, United States.

#415 PHOTOREACTIVATION IN BUDDING YEAST: A TEST OF HOW LESIONS IN DNA ARE DISCOVERED. Zhang, W, Heddle, JA. Department of Biology, York University, Toronto, ON, Canada.

#416 POLYMORPHISMS OF DNA REPAIR GENE XPD AND DNA DAMAGE OF VCM-EXPOSED WORKERS IN CHINA. Zhu, SM, Wang, AH, Xia, ZL. School of Public Health, Fudan University, Shanghai, China.

#417 DEATH PATHWAYS AND P53 RESPONSE AFTER INDUCTION OF DIFFERENT DNA LESIONS ON THE DOUBLE-HELIX. Zirnberger, LF¹, Roos, WP², Kaina, B², Menck, CFM¹. ¹Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil, ²Institute of Toxicology, Division of Applied Toxicology, University of Mainz, Mainz, Germany.

Monday, September 5, 2005
3:00 PM–5:15 PM
Seacliff A/B

EMERGING ISSUES SYMPOSIUM—MUTAGENESIS AND HUMAN DISEASE

ADVANCES IN HUMAN BIOMONITORING

Chairs: Beatrice Pool-Zobel, University Jena, Jena, Germany and
Lucia R. Ribeiro, School of Medicine, Botucatu-UNESP, Brazil

3:00 PM #418 ENVIRONMENTAL COLON CARCINOGENESIS: ASSESSING THE BALANCE OF EXPOSURE AND SUSCEPTIBILITY IN COLONOCYTES AND PERIPHERAL LEUCOCYTES—
A BIOMICS APPROACH

Beatrice Pool-Zobel, University Jena, Jena, Germany

3:20 PM #419 RELEVANCE OF BIOMARKERS OF INDIVIDUAL SUSCEPTIBILITY IN MOLECULAR EPIDEMIOLOGY STUDIES

Maria Dusinska, Slovak Medical University, Bratislava, Slovakia

3:40 PM #420 EFFECTS OF DIETARY COMPONENTS ON GENOMIC STABILITY IN HUMANS: RESULTS OF INTERVENTION TRIALS

Siegfried Knasmuller, University Vienna, Vienna, Austria

4:00 PM #421 ADVANCING AGE AMONG HEALTHY NONSMOKING MALES IS ASSOCIATED WITH REDUCED SPERM MOTILITY AND INCREASED FREQUENCIES OF SPERM WITH DNA FRAGMENTATION, CHROMOSOMAL ABNORMALITIES AND GENE MUTATIONS

Andrew J. Wyrobek, Lawrence Livermore National Laboratory, Livermore, CA, United States

4:15 PM #422 DIETARY AND SMOKING HABITS INFLUENCE ANTI-B[a]P-DNA ADDUCTS IN ENVIRONMENTALLY LOW EXPOSED HUMANS

Sofia Pavanello, University of Padova, Italy

4:30 PM #423 GENOTYPE AND PHENOTYPE OF GLUTATHIONE-S-TRANSFERASE PI IN PLACENTA OF MEXICAN WOMEN LIVING AT THE US-MEXICO BORDER AND RELATION TO PLACENTAL PAH-DNA ADDUCT LEVELS

Penelope J.E. Quintana, San Diego State University, San Diego, CA, United States

4:45 PM #424 CYTOGENIC BIOMARKERS AND HUMAN CANCER RISK

Hannu Norppa, Finnish Inst. of Occupational Health, Helsinki, Finland

5:00 PM #425 INVESTING IN GENETIC TOXICOLOGY RESEARCH IN AFRICA

Wagida A. Anwar, Ain Shams University, Cairo, Egypt

Monday, September 5, 2005
3:00 PM–5:00 PM
Grand Ballroom A

EMERGING ISSUES SYMPOSIUM—ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

GENOTOXICOLOGY OF AQUATIC SYSTEMS

Chairs: Gisella A. Umbuzeiro, CETESB, Sao Paolo, Brazil and
Yasunobu Aoki, NIES, Tsukuba, Ibaraki, Japan

3:00 PM #426 DEVELOPMENT OF IN VIVO MUTAGENICITY ASSAY SYSTEM USING rpsL TRANSGENIC ZEBRAFISH FOR MONITORING ENVIRONMENTAL MUTAGENS

Yausnobu Aoki, NIES, Tsukuba, Ibaraki Japan

3:20 PM #427 RELEVANCE OF THE SALMONELLA ASSAY IN WATER QUALITY EVALUATION: INDICATION OF AZO DYES AS IMPORTANT AQUATIC CONTAMINANTS

Gisella A. Umbuzeiro, CETESB, Sao Paolo, Brazil

3:40 PM #428 GENOTOXIC AND ECOTOXICOLOGICAL ASSESSMENT FOR DIAGNOSIS OF AQUATIC ECOSYSTEMS

Vera M. Vargas, FEPAM, Porto Alegre, Brazil

4:00 PM #429 GENOTOXICITY BIOMARKERS IN FISH AND BLUE MUSSELS: RESULTS FROM BEEP CRUISES

Claudia Bolognesi, NIRC, Genova, Italy

4:15 PM #430 GENOTOXICTY AND ACUTE TOXICITY ASSESSMENT IN AQUATIC ENVIRONMENTS

Richard M. Walmsley, Gentronix, Ltd., Manchester, UK

4:30 PM #431 GENOTOXICITY OF MICROCYSTIN-LR: IMPLICATION OF REACTIVE OXYGEN SPECIES

Metka Filipic, National Institute of Biology, Ljubljana, Slovenia

4:45 PM #432 EVALUATION OF THE TERATOGENIC AND MUTAGENIC POTENTIAL OF THALIDOMIDE IN THE SMALL FISH MODEL JAPANESE MEDAKA

Don G. Ennis, University of Louisiana, Lafayette, LA, United States

Monday, September 5, 2005
3:00 PM–5:15 PM
Bayview

EMERGING ISSUES SYMPOSIUM—MUTATIONAL MECHANISMS

MICROBIAL INFECTION AND MALIGNANCY

Chairs: Patricia Ostrosky-Wegman, UNAM, Mexico City, Mexico and
Julie Parsonnet, Stanford University, CA, United States

3:00 PM MICROBES AND MALIGNANCY

Julie Parsonnet, Stanford University, Stanford, CA, United States

3:20 PM #433 ADULT STEM CELL THERORY OF THE MULTI-STAGE, MULTI-MECHANISM MODEL OF CARCINOGENESIS: ROLE OF INFLAMATION ON THE PROMOTION OF INITIATED STEM CELLS

James E.Trosko, Michigan State University, East Lansing, MI, United States

3:40 PM #434 TUMOR VIRUSES AND THE “INTRACELLULAR IMMUNE SYSTEM”: ROLES OF TUMOR SUPPRESSOR PATHWAYS IN CONTROLLING VIRAL INFECTION

Patrick S. Moore, University of Pittsburgh, Pittsburgh, PA, United States

4:00 PM #435 HCMV-INDUCED GENETIC INSTABILITY: BRAIN TUMOR CASE-CONTROL STUDY

Randa El-Zein, M.D. Anderson Cancer Center, University of Texas, Houston, TX, United States

4:15 PM #436 EXPOSURE TO WOOD SMOKE AND GENETIC SUSCEPTIBILITY FOR CERVICAL NEOPASIA AMONG HPV-INFECTED WOMEN IN COLUMBIA

Carlos H. Sierra-Torres, Universidad del Cauca, Popayan, Columbia

4:30 PM #437 BIOREGULATION OF MICROBES FOR THE ATTENUATION OF MICROBE-ASSOCIATED MALIGNANCY

Ki Baik Hahm, Ajou University School of Medicine, Suwon, South Korea

4:45 PM #438 MECHANISMS OF INFECTION-INDUCED CARCINOGENESIS

Patricia Ostrosky-Wegman, UNAM, Mexico City, Mexico

5:00 PM #439 CYCLIN D-RB PATHWAY IS ALTERED DURING CONTINUOUS EXPOSURE OF MOLT-3 HUMAN LYMPHOBLASTOID CELLS TO THE ANTIRETROVIRAL DRUG ZIDOVUDINE (AZT)

Ofelia A. Olivero, National Cancer Institute, NIH, Bethesda, MD, United States

Monday, September 5, 2005
3:00 PM–5:00 PM
Seacliff C/D

ENVIRONMENTAL MUTAGENESIS/CARCINOGENESIS

MUTAGENIC CONSEQUENCES OF ENVIRONMENTAL EXPOSURES

Chairs: Tetsuya Kamataki, Hokkaido University, Hakodate, Japan and Emilio Rojas, UNAM, Mexico City, Mexico

3:00 PM #440 GENE AND ENVIRONMENT INTERACTION: GENETIC POLYMORPHISM OF CYP2A6 AS A DETERMINANT OF TOBACCO RELATED CANCER RISK

Tetsuya Kamataki, Hokkaido University, Hakodate, Japan

3:20 PM #441 ENVIRONMENTAL TRACE ELEMENTS ASSOCIATED WITH CANCER RISK IN LUCRECIA-RN/BRAZIL

Carlos F.S. Castro, Catholic University at Brasília, Taguatinga, Brazil

3:40 PM #442 GENOTOXIC CONSEQUENCES OF MEXICO CITY ATMOSPHERIC POLLUTION

Emilio Rojas, UNAM, Mexico City, Mexico

4:00 PM #443 4-OXO-2-HEXENAL IN COOKED FOODS AND DNA ADDUCT FORMATION IN MOUSE ORGANS AFTER ORAL ADMINISTRATION

Kazuaki Kawai, University of Occupational and Environmental Health, Kitakyushu, Fukuoka, Japan

4:15 PM #444 BIOLOGICAL PROPERTIES OF AMINOPHENYLNORHARMAN FORMED FROM NORHARMAN AND ANILINE

Yukari Totsuka, National Cancer Center Research Institute, Tokyo, Japan

4:30 PM #445 PHOTOTOXICITY INCLUDING PHOTOMUTAGENICITY OF THIRTEEN SELECTED POLYCHLORINATED BIPHENYLS (PCBS) IN Salmonella typhimurium TA102 AND HUMAN SKIN CELLS

Lei Wang, Jackson State University, Jackson, MS, United States

4:45 PM #446 ENHANCEMENT OF DNA REPAIR BY RADIO-PROTECTORS: IN VIVO STUDIES WITH ANIMAL MODELS

C.K.K. Nair, Bhabha, Atomic Research Centre, Mumbai, India

Monday, September 5, 2005
3:00 PM–5:00 PM
Grand Ballroom B

EMERGING ISSUES SYMPOSIUM—MUTATIONAL MECHANISMS

SPONTANEOUS MUTAGENESIS AND HYPERMUTATION

Chairs: Nancy Maizels, University of Washington, Seattle, WA, United States and Karen M. Vasquez, University of Texas M.D. Anderson Cancer Center, Science Park–Research Division, Smithville, TX, United States

3:00 PM #447 BIOCHEMICAL BASIS OF SOMATIC HYPERMUTATION

Myron F. Goodman, University of Southern California, Los Angeles, CA

3:20 PM #448 RECOMBINOGENIC DNA STRUCTURES IN IMMUNOGLOBULIN GENE DIVERSIFICATION AND ONCOGENESIS

Nancy Maizels, University of Washington, Seattle, WA, United States

3:40 PM #449 NON-B DNA STRUCTURE-INDUCED GENETIC INSTABILITY

Karen M. Vasquez, University of Texas M.D. Anderson Cancer Center, Science Park–Research Division, Smithville, TX, United States

4:00 PM #450 SPINDLE CHECKPOINT-DEPENDANT PHOSPHORYLATION OF BLM AND CHORMOSOME STABILITY

Yi Wang, Baylor University, Houston TX, United States

4:15 PM #451 SPONTANEOUS MUTAGENESIS IN MICE WITH A TARGETED DISRUPTION OF THE MutYH GENE

Teruhisa Tsuzuki, Kyushu University, Fukuoka, Japan

4:30 PM #452 ROLE OF THE CHECKPOINT IN POST-REPLICATION REPAIR PATHWAY

Mihoko K. Kai, Stanford University School of Medicine, Stanford, CA, United States

4:45 PM #453 NUCLEOTIDE EXCISION REPAIR TRIGGERS H2AX PHOSPHORYLATION INDEPENDANT OF DNA DOUBLE STRANDS BREAKS

Eli Hefner, University of California, San Francisco, CA, United States

Monday, September 5, 2005
5:00 PM–5:30 PM
Grand Ballroom Foyer

REFRESHMENT BREAK

Monday, September 5, 2005
5:30 PM–6:15 PM
Grand Ballroom

PLENARY LECTURE—JOHN S. MATTICK

Sponsored by National Institute of Environmental Health Sciences, Center for Rodent Genetics

Introduction

Liza Snow, Vice President of the Mutagenesis and Experimental Pathology Society of Australasia (MEPSA), Deakin University, Victoria, Australia

#454 RNA REGULATION: A NEW GENETICS

John S. Mattick, University of Queensland, Brisbane, Australia

Monday, September 5, 2005
6:30 PM–8:00 PM
Bayview

EMS BUSINESS MEETING AND AWARDS PRESENTATION

EMS Awards and Travel Awards will be presented.

<<Sunday<< - >>Tuesday>>