EMS 2002: Frontiers Beyond the Human Genome
April 27 - May 2, 2002
Anchorage, Alaska

Saturday
4/27/02

Sunday
4/28/02

Monday
4/29/02

Tuesday
4/30/02

Wednesday
5/01/02

Thursday
5/02/02

8:00am -
10:00am

Strategic Planning Committee Meeting

Cook Inlet Board Room

1:00pm -
5:00pm

EMS Council Meeting

Aspen Room

3:30pm -
7:00pm

Registration

Promenade

6:00pm -
7:30pm

Student Mixer/Reception

Chart Rooom

7:30am -
5:00pm

Registration

Promenade

7:00am -
8:30am

Breakfast Meetings

2003 Miami Beach Program Committee (First Meeting)

Birch Room

Nominating Committee

Prudhoe Bay Room

Student and Young Investigators: Professional Survival Skills

Spruce Room

8:30am -
12:30pm

Mitochondrial Damage in Aging and Carcinogenesis
{Sponsored by The Ellison Foundation}
Chairs, George Martin, University of Washington, and Douglas Wallace, Emory University

Alaska Room

8:30am

Gerantogens and Aging Genes
George Martin, University of Washington

9:00am

Mitochondrial Pathobiology in Mice and Man
Douglas Wallace, Emory University

9:30am

Point Mutations in Mitochondria
Giuseppe Attardi, California Institute of Technology

10:00am

Fidelity of Human DNA Polymerase-Gamma and Mitochondrial DNA Mutagenesis
William Copeland, National Institute of Environmental Health Sciences

10:30am

Coffee Break

Promenade

11:00am

Mitochondial DNA Repair and Changes with Aging and Cancer
Vilhelm Bohr, National Institute on Aging

11:30am

Age-Associated Accumulation of Mutations in Mouse Mitochondrial DNA
Magomed Khaidakov, US FDA, National Center for Toxicological Research

9:00am -
12:30pm

Exposure, Detection, and Toxicity
Chairs, Toby Rossman, New York University, and Ronald Snyder, Schering-Plough Research Institute

Bristol Bay Ballroom

9:00am

Introduction & How an Animal Model for Arsenic Carcinogenesis was Derived from Genetic Toxicology Studies
TG Rossman, AN Uddin, FJ Burns, and MC Bosland

Abstract 161

9:30am

Development of an Allele-Specific Amplification for Mouse P53 CC to TT Mutation, A Potential Biomarker for Estimating Skin Cancer Risk
BL Parsons, LH Couch, BJ Miller, and PC Howard

Abstract 146

9:45am

Measuring the Mitotic Index of Human Lymphocyte Cultures by Flow Cytometry Using the Histone 3-P mAb Biomarker
PA Muehlbauer and MJ Schuler

Abstract 130

10:00am

Flow Cytometric Assessment of Cell Cycle, Mitotic Index, and Aneuploidy in Human Lymphocyte Cultures Using the Histone 3-P mAB Biomarker
PA Muehlbauer and MJ Schuler

Abstract 131

10:15am

Flow Cytometric Technique for Measuring Cytogenetic Damage in Human Peripheral Blood Samples
SD Dertinger, DK Torous, NE Hall, FG Murante, SE Gleason, and CR Tometsko

Abstract 41

10:30am

Coffee Break

Promenade

11:00am

Study of Genotoxicity Associated with CYP450 Isozyme Induction Due to Liver Infection
RD Montero, L Serrano, VM Dávila, and A Plancarte

Abstract 127

11:15am

DNA Adduct in Nasal Mucosa: A New Marker in Human Studies on Airborne Carcinogens
S Bonassi, M Peluso, M Neri, A Munnia, E Taioli, S Garte, and M Buratti

Abstract 17

11:30am

Detection of Random Mutations In Situ
JF Davidson, LA Loeb, and JA Heddle

Abstract 35

11:45am

Frameshift Mutation Detected In Situ in Tumors
JR Stringer, MN Hersh, and SL Stringer

Abstract 194

12:00pm

The Fanconi Anaemia Protein FANCD2 Associates with Damaged DNA In Vivo
MS Meyn and W Wang

12:15pm

Cytotoxicity Measurements in Genetic Toxicology Assays: Further Evaluation & Summary
RD Snyder

Abstract 237

1:30pm -
5:30pm

Epithelial Genetic Instability: News from Where it Matters
Chairs, Richard J. Albertini, University of Vermont, and Raymond Monnat,University of Washington

Alaska Room

1:30pm

Introduction
Richard J. Albertini, University of Vermont

2:00pm

Epithelial Mutagenesis in Human Kidney: What's the 'Take Home'?
Raymond Monnat, University of Washington

2:30pm

Phylogeny of Cancer
Darryl Shibata, University of Southern California

3:00pm

Chromosomal Instability and Telomere Shortening in Early Gastrointestinal Tumors
Peter Rabinovitch, University of Washington

3:30pm

Coffee Break

Aleutian Room

4:00pm

Genomic Instability Secondary to Infection
Thomas Albrecht, University of Texas Medical Branch, Galveston

4:30pm

Human Mutation Load Assay (HMLA) Using the p53 Gene of Single Cells from Paraffin-Embedded Human Tissues
Steve Sommer, City of Hope

5:00pm

DNA Polymerase 1, an Enzyme that Can Preferentially Replicate Dupurinated DNA
Ulrich Hübscher, University of Zürich

1:30pm -
5:30pm

Exhibits Open and Posters on Exposure, Detection, and Toxicity

Aleutian Room

7:00pm -
8:00pm

Keynote Lecture: Mutations and Micronutrients
Bruce Ames, Children's Hospital Oakland Research Institute

Alaska Room

8:00pm -
9:30pm

Exhibits Open and Posters: Exposure, Detection, and Toxicity (Posters Attended)

Aleutian Room

7:30am -
5:00pm

Registration

Promenade

7:00am -
8:30am

Breakfast Meetings

Education and Student Membership Committee

Aspen Room

Finance Committee

Prince William Board Room

Future Meetings Committee

Prudhoe Bay Room

Public Relations and Communications Committee

Cook Inlet Board Room

Student Breakfast Meeting: Professional Survival Skills

Chart Room

8:30am -
12:30pm

Genetic Diversity and Disease
Chairs, JunJian Chen, US FDA, National Center for Toxicological Research, and Martyn Smith, University of California, Berkeley

Alaska Room

8:30am

Introduction
Fred Kadlubar, US FDA, National Center for Toxicological Research

9:00am

SNPing in the Human Genome
Debbie Nickerson, University of Washington

9:30am

Genetic Architecture and Molecular Mutagenesis of the Human Mitochondrial Genome
Junjiam Chen, US FDA, National Center for Toxicological Research

10:00am

Variations in DNA Repair are Extensive and Relevant to Individual Cancer Risks
Harvey Mohrenweiser, Lawrence Livermore National Laboratory

10:30am

Coffee Break

Promenade

11:00am

Gene Environmental Interactions in Breast and Colorectal Cancer
David Hunter, Harvard University

11:30am

Gene Environmental Interactions in Leukemia
Martyn Smith, University of California, Berkeley

8:30am -
12:00pm

Selected Platform Talks: DNA Damage and Repair
Chairs, Bea Singer, University of California, Berkeley, and William Bodell, University of California, San Francisco

Bristol Bay Ballroom

8:30am

Introduction
B Singer

9:00am

The Fidelitiy of Y Family DNA Polymerases: Functional and Mechanistic Implications
TA Kunkel

Abstract 97

9:15am

The Participation of Eukaryotic Polynucleotide Kinases in DNA Strand Breaks Repair
F Karimi-Busheri, C Whitehouse, K Caldecott, M Meijer, T Huang, D Young, and M Weinfeld

Abstract 88

9:30am

Human Apurinic/Apyrimidinic Endonuclease for the Assessment of Biological Functions
JC Shen and LA Loeb

Abstract 181

9:45am

Identification of a Novel Protein - Protein Interaction Between Two Multi-Functional Human DNA Repair Proteins, Xeroderma Pigmentosum-G, and Poly(ADP-Ribose)Polymerase
JM Pluth, AR Davalos, J Campisi, and PK Cooper

Abstract 149

10:00am

AP Endonuclease, PARP and XRCC1 Proteins Interact with a Truncated DNA Polymerase Beta, A DNA Repair Protein
S Banerjee and N Bhattacharyya

Abstract 9

10:15am

Analysis of Genetic Changes Involved in Carcinogen-Induced Malignant Transformation of a Human Fibroblast Cell Strain in Culture
JJ McCormick, MA Battle, S O'Reilly, SE Boley, J Zhang, and VM Maher

Abstract 117

10:30am

Coffee Break

Promenade

11:00am

Characterization of Cancer-Associated DNA Polymerase Beta Mutants
T Lang, D Starcevic, A Lomos-Gross, PM Glazer, and JB Sweasy

Abstract 100

11:15am

Identification of a Subset of Type II I-Compounds as Dinucleotides Containing 8,5'-cyclo-2'-deoxyadenosine
K Randerath, GD Zhou, RL Somers, JH Robbins, and PJ Brooks

Abstract 156

11:30am

Hot Spots for Chromosomal Instability in Human Cells
AJ Grosovsky, R Allen, L Ritter, and S Moore

Abstract 71

11:45am

Summary
B Bodell

12:15pm -
1:30pm

Business Meeting and Awards (Boxed Lunches Provided)

Spruce Room

1:30pm -
5:30pm

Double-Stranded Breaks: The Ultimate End Game
Chairs, Mike Resnick, National Institute of Environmental Health Sciences, and Larry Thompson, Lawrence Livermore National Laboratory

Alaska Room

1:30pm

Double-Strand Breaks: The Ends Justify the Means
Mike Resnick, National Institute of Environmental Health Sciences

2:00pm

Homologous Recombination and Genomic Integrity in Mammalian Cells
Maria Jasin, Sloan-Kettering

2:30pm

The Role of RAD51 Paralogs in Homologous Recombinational Repair
Larry Thompson, Lawrence Livermore National Laboratory

3:00pm

Arrested Replication as a Source for DNA Strand Breaks
Jim Cleaver, University of California, San Francisco

3:30pm

Coffee Break

Aleutian Room

4:00pm

Functions of RAD52 Group Proteins in Recombination and DNA Repair
Patrick Sung, University of San Antonio

4:30pm

Dynamic Interactions Between RAD52 Group Proteins in Living Cells
Roland Kanaar, Erasmus University

5:00pm

Protein Interactions in Recombinational Repair in Human Cells
David Schield, Lawrence Berkeley National Laboratory

1:30pm -
5:30pm

Exhibits Open and Posters on DNA Damage and Repair

Aleutian Room

7:00pm -
8:00pm

Keynote Lecture: Genomic Views of Human History
Mary-Claire King, University of Washington

Alaska Room

8:00pm -
9:30pm

Exhibits Open and Posters on DNA Damage and Repair (Posters Attended)

Aleutian Room

7:00am -
8:30am

Breakfast Meetings

DNA Repair Group

Katmai Room

Molecular Epidemiology Group

Dillingham Room

• Introduction - William Au
• Special topic conference series on colon cancer - Marti Veigl
• New Initiatives for Assessing Exposure to Environmental Mutagens - Stefano Bonassi
• Organization of the Molecular Epidemiology Workshop in Miami, 2003 - Fred Kadlubar
• The 4th International Conference on Environmental Mutagens in Human Populations, Brazil, May 2003 - Lucia Ribeiro and William Au

Open discussions for opportunities and initiatives - William Au

Risk Assessment Group

Cook Inlet Board Room

Exhibitors' Breakfast

Prince William Board Room

8:30am -
6:00pm

High-Speed Catamaran Glacier Tour

7:00pm -
10:00pm

Banquet Dinner: Hilton Hotel
EMS and Hollaender Awards
Carl Hild: The Alaskan Wilderness

Alaska Ballroom

7:30am -
5:00pm

Registration

Promenade

7:00am -
8:30am

Breakfast Meetings

Awards and Honors Committee

Prudhoe Bay Room

Membership and Professional Development Committee

Willow Room

Organization Committee

Cook Inlet Board Room

Germ-Cells/Stem Cells/Human Genetics Group

Birch Room

8:30am -
12:30pm

Complex DNA Lesions: Repair and Mutagenesis
Chairs, Peter Glazer, Yale University, and Robb Moses, Oregon Health Sciences University

Alaska Room

8:30am

Mutagenesis and Recombination Induced by DNA Triple Helix Formation
Peter Glazer, Yale University

9:00am

Unusual DNA Conformations are Mutagenic: Implications for Hereditary Neurological Diseases and Polycystic Kidney Disease
Robert Wells, Texas A&M University

9:30am

CAG Expansion in Human Disease is Caused by Stabilization of Hairpins with a Mismatch Repair Complex During Gap Repair
Cynthia McMurrary, Mayo Clinic

10:00am

Fanconi Anemia Gene Function in Repair of Interstrand Crosslinks
Robb Moses, Oregon Health and Science University

10:30am

Coffee Break

Promenade

11:00am

Recognition and Processing of Interstrand Cross-Links in Mammalian Cells
Randy Legarski, MD Anderson

11:30am

Break-and-Repair Pathways of Immunoglobulin Gene Switch Recombination and Somatic Hypermutation
Nancy Maizels, University of Washington

12:00pm

ADP-Ribosylation of DNA by Pierisin, an Apoptogenic Peptide from Cabbage Butterfly
Takashi Sugimura, National Cancer Institute, Japan

9:00am -
12:30pm

Selected Platform Talks: Health Effects and Epidemiology
Chairs, Curt Harris, National Institutes of Health, and Miriam Poirier, National Institutes of Health

Bristol Bay Ballroom

9:00am

Effects of the Anti-Mutagens Vanillin and Cinnamaldehyde on Spontaneous Mutation in E. coli LacI Strains and on Global Gene Expression in Salmonella TA104 and Human HEPG2 Cells
DT Shaughnessy and DM DeMarini

Abstract 179

9:15am

First Trimester of Pregnancy Exposure to Tobacco Smoke and Risk of Childhood CNS Tumors: A Meta-Analysis
AMMC Gontijo and TR Einarson

Abstract 68

9:30am

Maternal Exposure to Glycol Ethers: Clinical and Cytogenetic Findings
RA El-Zein, SZ Abdel-Rahman, DL Morris, and MS Legator

Abstract 47

9:45am

Effect of Individual Glutathione S-Transferase GSTM1, GSTT1, and GSTP1 Polymorphisms on the Genotoxicity of Hydroquinone
J Gasper, MC Silva, A Faber, and J Rueff

Abstract 56

10:00am

Genetic Polymorphism of Glutathione S-Transferase (GST) A1 That Predicts Low Hepatic Expression of GSTA1 is Associated with Increased Survival of Brreast Cancer Following Cyclophosphamide Chemotherapy
B Coles, L Joseph, C Sweeney, A Stone, M Fares, L Hutchins, and C Ambrosone

Abstract 32

10:15am

Developmental Pattern of O6-Methylguanine-DNA Methyltransferase Activity in Human Brain and Implications for Neurocarcinogenesis
JR Silber, MS Bobola, and A Blank

10:30am

Coffee Break

Promenade

11:00am

Mutant Frequencies and Spectra of Mutations in the Liver LacI and CII Genes of Lambda/LacI Transgenic Mice Treated as Neonates with 4-Aminobiphenyl
T Chen, RA Miittelstaedt, RR Heflich, MM Moore, and BL Parsons

Abstract 21

11:15am

Residual Mutagenicity of the Alaskan Oil Spill Organics
LD Claxton, S Warren, F Kremer, and JW Short

Abstract 28

11:30am

Toxicogenomics and the Chemical Effects in Biological Systems (CEBS) Knowledge Base
MD Waters, RW Tennant, and the Staff of the NTC

Abstract 218

11:45am

Spontaneous Tandem-Base Mutations Show Tissue Specific Frequencies, Accumulate with Age and Occur Preferentially as G:C to T:A and G:G to T:T Mutations at Specific Sites
K Hill, J Wang, A Halangoda, and S Sommer

Abstract 78

12:00pm

Persistent Biological Effects from Exposure to Environmental Mutagens
WW Au

Abstract 7

12:15pm

Predicting Treatment Toxicity in Cancer Patients from Microarrays
G Chu, VG Tusher, K Rieger, WJ Hong, J Tang, and R Tibshirani

Abstract 25

1:30pm -
5:30pm

When Polymerases are Arrested, Who is at Fault, and What are the Options?
Chairs, Joann Sweasy, Yale University, and Phil Hanawalt, Stanford University

Alaska Room

1:30pm

Introduction
Phil Hanawalt, Stanford University

2:00pm

Amino Acid Residues Distant from the Active Site Govern Fidelity in DNA Polymerase Beta
Joann Sweasy, Yale University

2:30pm

Crystal Structure of a Lesion Bypass Polymerase: Implications for Fidelity and Processivity
Janice Pata, Yale University

3:00pm

Why Do Polymerases Pause and Misincorporate at 06-alkyl G: Kinetic Analysis with Replicative Polymerases?
Fred Guengerich, Vanderbilt University

3:30pm

Coffee Break

Aleutian Room

4:00pm

Endogenous DNA Damage and Mutagenesis: Role of Polymerase and the 3' to 5' Exonuclease in Mutations; Avoidance and DNA Synthesis
Kristin Eckert, Pennsylvania State University

4:30pm

Human DNA Polymerase ETA (XPV): Interactions with Damaged DNA and Other Protein Factors
Fumio Hanaoka, Osaka University

5:00pm

RNA Polymerase Behavior at Lesion Sites in DNA: Implications for Transcription Coupled DNA Repair
Silvia Tornaletti, Stanford University

1:30pm -
5:30pm

Exhibits Open and Posters on Health Effects and Epidemiology

Aleutian Room

7:00pm -
8:00pm

Keynote Lecture: Viral Quasispecies and Error Threshold
Manfred Eigen, Max-Planck Institut

Alaska Room

8:00pm -
9:30pm

Posters on Health Effects and Epidemiology (Posters Attended)

Aleutian Room

7:30am -
12:00pm

Registration

Promenade

7:00am -
8:30am

Breakfast Meetings

2003 Miami Beach Program Committee (Second Meeting)

Birch Room

Hollaender Committee

Prince William Board Room

Technology Group

Prudhoe Bay Room

Transgenic and In Vivo Mutagenesis Interest Group

Chart Room

EMS Executive Board

Cook Inlet Board Room

8:30am -
12:30pm

New Perspectives from Functional Genomics and Proteomics
Chairs, Leona Samson, Massachussetts Institute of Technology, and Cynthia Afshari, National Institute of Environmental Health Sciences

Alaska Room

8:30am

Functional Genomics and Alkylation Resistance
Leona Samson, Massachussetts Institute of Technology

9:00am

Probing the Mechanism of Action of Non-Mutagenic Carcinogens with cDNA Microarrays
Cynthia Afshari, National Institute of Environmental Health Sciences

9:30am

Transcriptional Responses to Low-Dose Radiation Exposure - A Model for Genotoxic Stress Responses at Sub-Toxic Doses
Al Fornace, National Institutes of Health

10:00am

Proteomics without Polyacrylamide: Qualitative and Quantitative Analysis
David Goodlett, Institute for Systems Biology

10:30am

Coffee Break

Promenade

11:00am

Loss of Genomic Integrity in Pre-Neoplastic Cells
Thea Tlsty, University of California, San Francisco

11:30pm

Recombineering: Applications for Functional Genomics in the Post-genome Era
Neil Copeland, National Cancer Institute

9:00am -
12:30pm

Selected Platform Talks: Mechanisms of Mutagenesis
Chairs, Tom Kunkel, National Institute of Environmental Health Sciences, and Roel Schaaper, National Institute of Environmental Health Sciences

Bristol Bay Ballroom

9:00am

Introduction
R Schaaper

9:30am

Biochemical Characterization of Muring DNA Polymerase Iota and its Copmparison to Human Polymerase Iota
EG Frank, JP McDonald, and R Woodgate

Abstract 53

9:45am

Human DNA Polymerase Epsilon Localizes with DNA Replication and PCNA in Late, But Not Early S Phase
S Linn and J Fuss

Abstract 104

10:00am

Human DNA Polymerase Beta Catalyzes Expansion of CTG/CAG Trinucleotide Repeats at Strand Breaks
MJ Hartenstine, MF Goodman, and J Petruska

Abstract 74

10:15am

High Throughput In Vitro Production and Screening of Polymerases
EG Glick, JPA Anderson, and LA Loeb

Abstract 64

10:30am

Coffee Break

Promenade

11:00am

Error Prone Translesion Synthesis Past the Primary Acrolein Derived Hydroxypropano Deoxyguanosine Adducts in Mammallian Cells
M Kanuri, IG Minko, LV Nechev, TM Harris, CM Harris, and RS Lloyd

Abstract 86

11:15am

Effect of Loss of Translesion Synthesis Polymerases or a Protein Involved in Damage Avoidance on the Frequency of Mutations Induced in Human Cells by Carcinogens
VM Maher, Z Li, XD Wang, K McNally, JJ McCormick, CW Lawrence, and W Xiao

Abstract 110

11:30am

Involvement of DNA Pol IV (DINB) of Escherichia coli in Chemically-Induced Mutagenesis
T Nohmi, SR Kim, K Matsui, P Gruz, M Shimizu, and M Yamada

Abstract 137

11:45am

Identification of In Vivo Mutants from Mouse Splenic Lymphocytes by Single Burst Analysis of the Forward Mutational Assay for Gene A of PhiX174
CR Valentine, JL Raney, JG Shaddock, and VN Dobrovolsky

Abstract 207

12:00pm

Quantitative Characterization of Aberrant Splicing in Humans
A Skandalis, E Uribe, and P Ninniss

Abstract 186

12:15pm

Summary
T Kunkel

1:30pm -
5:30pm

Nanotechnology and Single-Molecules
Chairs, John Essigmann, Massachussetts Institute of Technology, and Sidney Aaron, Pharmacia

Alaska Room

1:30pm

Introduction
John Essigmann, Massachussetts Institute of Technology

2:00pm

Biomolecular Diagnostics of Nucleic Acid in Single Cells In Situ
Jim Tucker, Lawrence Livermore National Laboratory

2:30pm

Chemosensors: Nano-Receptors in the Service of Analytical Chemistry
Anthony Czarnik, Sensors for Medicine & Science, Inc.

3:00pm

Canary B-Cell Sensor for Rapid Identification of Pathogens
Eric Schwoebel, Lincoln Laboratories, Massachussetts Institute of Technology

3:30pm

Coffee Break

Promenade

4:00pm

Single-Molecule DNA Sequencing
Rudolf Rigler, Karolinska Institute

1:30pm -
5:30pm

Posters on Mechanisms of Mutagenesis

Aleutian Room

4:30pm -
6:00pm

EMS Council Meeting

Aspen Room

7:00pm -
8:00pm

Keynote Lecture: Genomics, Proteomics, and Systems Biology
Leroy Hood, Institute for Systems Biology

Alaska Room

8:00pm -
9:30pm

Posters on Mechanisms of Mutagenesis (Posters Attended)

Aleutian Room