The Annual EMS Meeting:

March 27-April 1, 1999 Washington, D.C.

Meeting Program

James Felton, Program Chair

Saturday, March 27, 1999

1:00pm -
5:00pm EMS Council meeting

6:30pm -
8:00pm Student Poster Session
{Sponsored by the Genetic Toxicology Association}
This year marks our Second Annual Student Poster Session. During this session, students and new investigators will present the same posters they have submitted to the EMS meeting and each entry will be considered for the Student Poster Award. This award is awarded by the EMS Education Committee based on the votes of your fellow students and new investigators who judge the scientific merit of each presentation. The only requirements for qualification are that the work is presented by a student or new investigator, the work is also presented as part of the main EMS meeting, and posters must be set up by 6:15 PM at the Student Reception, students and new investigators competing for the Student Poster Award must be present at the reception. All entries must be received by January 22, 1999.

6:30pm -
10:00pm Student Reception
{Sponsored by the Genetic Toxicology Association}
The evening will begin with the Student Poster Session. During this time, a buffet dinner of pizza, beer and sodas is served. Following the poster session we will hear from our evening's speakers who will focus on the future of life and environmental sciences and how to prepare ourselves for a career in these fields. We will also award the EMS Education Committee Award to a senior EMS member in recognition of their commitment to the education and mentoring of students. After the presentations, students and new investigators get a chance to talk with the presenters, the senior EMS members and their friends.

6:30pm Students, Investigators, and guests arrive. Posters are viewed and the students vote for the best poster presentation.

7:50pm Switch rooms from Poster session venue to presentation venue.

8:00pm Dr. Alison Director welcomes the attendees and introduces Dr. Nyquist

8:05pm Presentation by Dr. Judith Nyquist, Director of Associateship Programs, National Research Council

8:30pm Presentation by Dr. Kathleen Hill, EMS New Investigator Member

8:45pm Presentation by Lidia Cosentino and Roy Swiger, EMS Student Members

9:00pm Presentation of the EMS Education Committee Award

Sunday, March 28, 1999

7:00am -
8:00am Student Breakfast Workshop
The fourth annual Student Breakfast will focus on a number of popular and useful topics that students can discuss with the experts attending the meeting. Traditionally, the breakfast provides a relaxed and enjoyable atmosphere for students and new investigators to meet the experts of a particular field of study, ask questions, discuss techniques, and learn about educational and employment opportunities. This year's topics include DNA repair, genetic instability and job search strategies. Please pick your top 3 choices when filling out the response form.

7:30am Continental Breakfast Upper Lobby

8:30am -
11:30am Somatic Mutations In Vivo
Presiding: John Heddle, York University, Toronto Presidential Ballroom
Although the involvement of somatic mutation in cancer is certain, the origin of these mutations is not. The development of transgenic mice containing shuttle vectors has made the experimental investigation of this problem possible in a way that we could only dream about before. Many of the results obtained are surprising: spontaneous somatic mutations are very common; many arise very early in life rather than late in life, as had often been supposed; there is often a long lag time before the mutant frequencies are maximal after treatment; many carcinogens induce only a small increase in the mutant frequency in the target tissue; and so on. Our understanding of how to use these tools, the use of the tools, and the creation of new tools are all advancing at the same time. So much is happening that no one symposium can include it all.

8:30am gpt-delta Mouse as a New Research Tool for In Vivo Mutagenesis
T. Nohmi, Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan

9:00am Gene Mutation in lacl Transgenic Rats: Comparison of lacl in Splenocytes and Target Organs and Hprt in Splenocytes
D. Casciano, National Center for Toxicology Research, Jefferson, AR

9:30am Preferential Repair of Endogenous Loci During Low Chronic Treatment
L. Cosentino, York University, Toronto, Canada

10:00am Coffee Break

10:30am Organ-Specific Mutation Frequencies and Spectra in Aging Mice with Defects in Genome Stability systems
J. Vijg, Beth Israel Hospital, Boston, MA

11:00am Loss of Functional Heterozygosity in a Mouse Model: Implications for Human Disease
J. Tischfield, Indiana University School of Medicine, Indianapolis, IN

9:00am -
5:00pm Watershed and Drinking Water Toxicology: Studies at the EPA
Organized by: Thomas Hughes and Larry Claxton, Environmental Carcinogenesis Division Divison (ECD), EPA, Research Triangle Park, NC South American AB
Chair, Thomas Hughes

9:00am Introduction: Short Term Tests That Can Detect Genotoxicants in Watersheds and Drinking Water
Thomas Hughes, ECD, RTP

9:30am Sediments in our Rivers and Estuaries - Are They Toxic?
Michael Lewis, Gulf Ecology Division, Gulf Breeze, FL

10:00am Coffee Break

10:30am Tumors in Fish as Indicators of Carcinogens in our Watersheds
George Gardner, Atlantic Ecology Division, Naragansett, RI

11:00am Laboratory Studies with Fish as Indicators of Toxicity
Rodney Johnson, Mid-Continent Ecology Division, Duluth, MN

11:30am Lunch

Chair, Larry Claxton, Director of ECD, RTP

1:00pm Introduction: Drinking Water Research at the NHEERL, EPA, RTP, NC
Larry Reiter, Director of the National Health and Environmental Effects Research Lab (NHEERL), RTP

1:30pm Searching for Water-Borne Carcinogens and Their Sources
Larry Claxton, Director of ECD, RTP

2:00pm The Carcinogenicity of Dichloroacetic Acid in Drinking Water
Tony DeAngelo, ECD, RTP

2:30pm Construction of a BBDR Cancer Model for the Water Disinfectant Dicholoracetic Acid
Jim Rabinowitz, ECD, RTP

3:00pm Break

3:30pm Arsenic in Drinking Water: More Than One Proposed Mechanism for Cancer
Marc Mass, ECD, RTP

4:00pm The Micronucleus Bioassay as a Potential Biomarker for Toxicity of Drinking Water
Andy Kligerman, ECD, RTP

4:30pm 2001 - Research Data Needed for the New Drinking Water Regulations
Rita Schoeny, EPA HQ, Washington, DC

5:00pm Adjourn

1:00pm -
4:00pm Workshop: Interpretation of Mutation Data for Cancer Risk Assessment
{Sponsored by Baxter Healthcare}
Discussion Leaders: Martha Moore, US-EPA, Research Triangle Park, and Dan Casciano, National Center for Toxicology Research, Jefferson, AR Federal B Room
1:00pm Introduction
Martha Moore

1:10pm Overview of the New EPA Cancer Risk Assessment Guidelines - With an Emphasis on Use of Mode of Action Information
Jim Cogliano

1:40pm Kinetics of Induction of DNA Adducts, Cell Proliferation and Gene Mutations in MutaMice Treated with 5,9-Dimethyldibenzo(c,g)carbazole
Veronique Thybaud

2:10pm Integration of Genotoxicity Data for Trichloroethylene and its Metabolites: Implications for Risk Assessment
Martha Moore

2:40pm Coffee Break

2:50pm Epidemiological/Human Data: How it Informs the Trichloroethylene Risk Assessment
Cheryl Scott

3:10pm Panel Discussion
Dan Casciano, Chair

1:00pm -
4:00pm New Technologies in Cytogenetics and Gene Expression
{Sponsored by Chrysalis International Preclinical Services}
Chair: Joe Gray, U.C. San Francisco Presidential Ballroom
1:00pm Introduction
Joe Gray

1:15pm Prospects for Mutation Detection Using FISH
David Ward, Yale University

1:55pm Microarray Analysis of Genomic Aberrations in Cancer
Donna Albertson, U.C. San Francisco

2:35pm Coffee Break

2:55pm Expression Array Analysis of DNA Repair Genes
Andy Wyrobek, Lawrence Livermore National Laboratory

3:35pm FISH for Interphase Translocation Detection
[Speaker to be announced]

4:30pm -
6:30pm Poster Session I Discussion Session
{Sponsored by Taconic Transgenic Division, Taconic Farms, Inc.} Senate Room / Federal A Room

7:30pm -
9:00pm Plenary Talk: Nuclear Structure and DNA Organization and Function
Speaker: Don Coffey, Johns Hopkins University Presidential Ballroom

8:30pm -
10:00pm Reception
{Sponsored by BioReliance and Covance Laboratories, Inc.} Congressional/Senate

Monday, March 29, 1999

7:30am Continental Breakfast Congressional/Senate

8:30am -
11:30am Chromosome Structure and Function
{Sponsored by Bristol-Myers Squibb Pharmaceutical Research Company}
Chair: Peter Stambrook, University of Cincinnati Presidential Ballroom
8:30am Telomere Dynamics in Yeast
Arthur Lustig, Tulane University

9:10am P53 Mutation, Centrosome Hyperamplification and Chromosome Instability in Human Cancer
Kenji Fukasawa, University of Cincinnati

9:50am Coffee Break

10:10am Mitotic Checkpoints and Human Chromosome Segregation
Frank McKeon, Harvard University

10:50am Mammalian Centromeres and Artificial Chromosomes
Howard Cooke, Edinburgh

11:30am -
12:30pm Awards

12:30pm -
1:30pm EMS Business Meeting

1:30pm -
4:30pm Linking Cytogenetic and Somatic Cell Mutation Biomarkers to Future Cancer Risk
{Sponsored by The Procter & Gamble Company}
Chairs: Nathaniel Rothman, NCI, and Douglas Bell, NIEHS South American AB
1:30pm Introduction: A Molecular Epidemiologic Perspective
Nathaniel Rothman, NIH/NCI

1:50pm Chromosomal Damage in Peripheral Blood Lymphocytes and Risk of Cancer in Humans
Stefano Bonassi, Dept. of Environmental Epidemiology, Instituto Nazionale per la Ricerca sul Cancro, Genova, Italy

2:25pm Potential Applications of Mutational Biomarkers in Prospective Epidemiology Studies
Martyn Smith, School of Public Health, Division of Environmental Health Sciences, University of California, Berkeley

3:00pm Biology and Below Threshold Mutation Assays
Vincent L. Wilson, Laboratory of Environmental and Human Toxicology, Institute for Environmental Studies, Institute for Mutagenesis, Louisiana State University

3:30pm MutEx/ACB-PCR for the Detection of Point Mutations in Somatic Cells
Barbara L. Parsons, Division of Genetic and Reproductive Toxicology, National Center for Toxicological Research

4:00pm Discussant
Richard Albertini, University of Vermont, Genetic Toxicology Laboratory

1:30pm -
4:30pm Genetox Test Procedures
{Sponsored by Olympus Optical Co., Ltd.}
Chairs: Jim MacGregor, FDA, and David Kirkland, Covance Laboratories Presidential Ballroom
1:30pm In Vitro Micronucleus Test
Micheline Kirsch-Volders, Vrije Universiteit Brussel

1:50pm Photochemical Induced Mutation and Clastogenicity
Elmar Gocke, F. Hoffman-La Roche, Ltd., Basel

2:10pm Mouse Lymphoma
Martha Moore, US-EPA, Research Triangle Park

2:30pm Cytotoxicity in the Chromosomal Aberration Test
Sheila Galloway, Merck Research Laboratories

2:40pm Comet Assay
Ray Tice, Integrated Laboratory Systems, Research Triangle Park

3:00pm Coffee Break

3:20pm In Vivo Micronucleus Test
Makoto Hayashi, National Institute of Health Services, Tokyo

3:40pm In Vivo Transgenic Mutation Assays
John Heddle, York University, Toronto

4:00pm Identification of Adducts
David Phillip, Institute of Cancer Research, Sutton, Surrey, UK

4:15pm Statistics in Genotoxicity Testing
David Lovell, Pfizer Central Research, Sandwich, Kent, UK

4:30pm -
6:30pm Poster Session II Discussion Session
{Sponsored by R.W. Johnson Pharmaceutical Research Institute} Congressional/Senate / Federal A Room

7:30pm -
8:30pm Public Lecture: Soft Drinks: A Good Part of America
Richard Adamson, National Soft Drink Association, Washington, DC Presidential Ballroom

Tuesday, March 30, 1999

7:30am Continental Breakfast
{Sponsored by Covance Laboratories, Inc.} Congressional/Senate / Federal A Room

8:30am -
11:30am The Continual Maintenance of Genomic Integrity: The Base Excision Repair Pathway
Chair: David M. Wilson, III, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory Presidential Ballroom
8:30am The Continual Maintenance of Genomic Integrity: The Base Excision Repair Pathway
David M. Wilson, III

8:50am Crystal Structures and Mechanistic Insights for Human and E. coli 3-Methyladenine DNA Glycoyslase
Tom Ellenberger, Dept. of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

9:30am Two Pathways of Base Excision Repair: a DNA Polymerase Beta-Dependent Pathway and a PCNA-Dependent Pathway
Yoshihiro Matsumoto, Dept. of Radiation Oncology, Fox Chase Cancer Center, Philadelphia

10:10am Coffee Break

10:30am DNA Ligases in Base Excision Repair
Alan Tomkinson, Dept. of Molecular Medicine, Institute of Biotechnology, San Antonio

11:10am Use of DNA Base Excision Repair Genes in Gene Therapy; Translational Applications
Mark R. Kelley, Dept. of Pediatrics, Wells Center for Pediatric Research, James Whitcomb Riley Hospital for Children, Indianapolis

8:30am -
11:30am Risk Assessment Principles
{Sponsored by Eli Lilly and Co.}
Organized by: Martha Moore, US Environmental Protection Agency, Research Triangle Park South American AB
8:30am Risk Assessment Principles
John Vandenberg, US EPA, RTP, NC

9:00am Formaldehyde Cancer Risk Assessment: Combining Mutagenic and Cytotoxic Modes of Action
Rory Conolly, CIIT, RTP, NC

10:00am Coffee Break

10:15am Incorporation of Mechanistic Information into Cancer Risk Assessment: Dioxin
Justin Teeguarden, University of Wisconsin, Madison

11:00am Panel Discussion

12:00pm -
1:00pm Plenary Talk: Using Transgenic Mice to Understand the Mode of Action of Carcinogens
{Sponsored by G.D. Searle and Company}
Frank Gonzalez, National Cancer Institute Presidential Ballroom

Free Afternoon
Things to see and do in Washington, DC

7:00pm -
~10:00pm Odyssey Cruise
(First bus from the Capital Hilton (16th & K St. entrance) to the Odyssey leaves at 6:00 pm.)
Cruise beyond your expectations on the ship voted "Outstanding vessel of the year" by Maritime Reporter. The Odyssey is the only ship of its kind in the U.S. Its unique design allows travel beneath the historic bridges spanning the Potomac River. Glass atrium dining rooms offer spectacular panoramic views of the Nation's greatest monuments. The quarter mile of open-air perimeter deck is perfect for watching the District drift slowly by. However, if sight-seeing is not where your interest lies, you can dance the night away to the refined entertainment in the main dining area, while enjoying the distinctive menu complemented by a wine list that has won Wine Spectator magazine's Award of Excellence four years running. Nowhere else can you find a combination of sumptuous cuisine, attentive service, monumental views and a memorable setting that only the Odyssey cruising vessel can provide. This special event is included in your registration fee. For additional tickets, see the registration form, or tickets can be purchased on site.

Wednesday, March 31, 1999

7:30am Special Interest Breakfasts South American A / South American B / Federal B Room

8:30am -
12:10pm Mutator Phenotype in Cancer
Chair: Snorri Thorgeirsson, National Cancer Institute Presidential Ballroom
8:30am Gastrointestinal Cancer of the Microsatellite Mutator Phenotype
Dr. Manuel Perucho, The Burnham Institute, La Jolla Cancer Research Center

9:10am Tissue-Specific Accumulation of Point Mutations and Genome Rearrangements in Aging Mice: Role of DNA Damage Processing and Repair
Dr. Jan Vijg, Harvard University

9:50am Origins of a Mutator Phenotype of Cancer
Dr. Lawrence Loeb, Gottstein Memorial Cancer Research Laboratory, Dept. of Pathology, University of Washington, Seattle

10:30am Coffee Break {Sponsored by Chrysalis International Preclinical Services}

10:50am Mutation Research Award Lecture: Studies of DNA Replication Fidelity and DNA Mismatch Repair
Dr. Thomas A. Kunkel, Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park

11:30am [Title to be announced]
Dr. Daniel P. Cahill, Research Laboratories, Johns Hopkins Oncology Center, Baltimore, MD

1:30pm -
4:30pm Common Diseases: Is it the Genes or the Exposures?
{Sponsored by Elsevier Science BV}
Chair: Harvey Mohrenweiser, Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory Presidential Ballroom
1:30pm Variation as a Tool for Understanding Biology: A Key Element in the Functional Genomic Era
Harvey Mohrenweiser

2:10pm Pharmacogenetics and Individualization of Therapy
Richard Weinshilboum, Department of Pharmacology, Mayo Medical School / Mayo Clinic

2:50pm Coffee Break

3:10pm Cardiovascular Disease: A Case Study of a Disease with Multifactorial Inheritance and Genetic Susceptibility
Mike Province, Division of Biostatistics, Washington University School of Medicine, St. Louis

3:50pm Screening for Genetic Variation and Disease Susceptibility: Promises, Pitfalls and Reality
David Hunter, Dept. of Epidemiology, Harvard School of Public Health

4:30pm -
6:30pm Poster Session III Discussion Session
{Sponsored by Astra Pharmaceuticals, L.P.} Senate Room / Federal A Room

7:30pm -
10:20pm Structural and Biological Consequences of DNA Modification
{Sponsored by Merck & Co.}
Chairs: William Baird, Oregon State University, and Anthony Dipple, National Cancer Institute Presidential Ballroom
7:30pm Introduction to DNA Adducts and Their Repair
William Baird

7:40pm DNA Adduct Structures in Relation to Biological Effects
Nick Geacintov, New York University

8:20pm Targeted Gene Knockouts Mediated by Triple Helix Forming Oligonucleotides
Michael Seidman, CODON, Gaithersburg, MD

9:00pm PAH Adduct Induced Mutations and Effects on Cell Cycle
Tony Dipple

9:40pm Site Specific Mutagenesis with Carcinogen Adducts and Oxidative Damage
John Essigmann, MIT

Thursday, April 1, 1999

7:30am Special Interest Breakfasts Federal A Room / Federal B Room

8:30am -
9:30am Plenary Talk: Predicting Protein Structure from DNA Sequence
John Moult, CARP, Rockville, MD Presidential Ballroom

9:30am -
12:30pm DNA Repair: Structural and Functional Studies
Chair: William F. Morgan Presidential Ballroom
9:30am Double-Strand Break Repair and At-Risk Motifs (ARMS) as Factors in Genome Stability
Michael A. Resnick, Head, Chromosome Stability Group, National Institute of Environmental Health Sciences, NIH, Research Triangle Park

10:10am Role of DNA-PK in DNA Damage Repair and Lymphomagenesis
Gloria C. Li, Dept. Medical Physics, Memorial Sloan Kettering Cancer Center

10:50am Structure-Function Studies of the Human Mre11/Rad50 Complex
James P. Carney, Lawrence Berkeley National Laboratory, Life Sciences Division

11:50am Crystallographic Structures of DNA Repair Complexes
John A. Tainer, Scripps Research Institute, Dept. of Molecular Biology, La Jolla

8:00am -
3:00pm Continuing Education Course on the New EPA Cancer Risk Assessment Guidelines Pan American Room
The U.S. Environmental Protection Agency has proposed significant changes to the cancer risk assessment guidelines and is in the process of implementing these changes. As part of the Annual Meeting, the Environmental Mutagen Society is sponsoring a continuing education course to allow those in attendance to get an in depth look at the proposed changes and to learn how these will affect the risk assessment process. This course will be taught by Jim Cogliano and associates from EPA. This is a new course being developed within the Agency to train risk assessment personnel using exercises and examples. Since the new guidelines represent a significant shift in the EPA's approach to regulating carcinogens, understanding the new guidelines and the Agency's vision for their application will be valuable for both new and established members of the EMS. Those interested in participating in the class will need to enroll on the Meeting Registration Form and also contact David Eastmond to reserve a place. Participants should expect to stay for the entire course. To facilitate interaction between the attendees and the presenters, the class size will be limited with those registering early given highest prority. To reserve a spot or if you have additional questions, contact David Eastmond at (909) 787-4497 or david.eastmond@ucr.edu.

1:00pm -
6:00pm EMS Council meeting